PMID- 32111666
OWN - NLM
STAT- MEDLINE
DCOM- 20210128
LR  - 20240328
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 32
IP  - 5
DP  - 2020 May
TI  - The Arabidopsis Plastidial Glucose-6-Phosphate Transporter GPT1 is Dually 
      Targeted to Peroxisomes via the Endoplasmic Reticulum.
PG  - 1703-1726
LID - 10.1105/tpc.19.00959 [doi]
AB  - Studies on Glucose-6-phosphate (G6P)/phosphate translocator isoforms GPT1 and 
      GPT2 reported the viability of Arabidopsis (Arabidopsis thaliana) gpt2 mutants, 
      whereas heterozygous gpt1 mutants exhibited a variety of defects during 
      fertilization/seed set, indicating that GPT1 is essential for this process. Among 
      other functions, GPT1 was shown to be important for pollen and embryo-sac 
      development. Because our previous work on the irreversible part of the oxidative 
      pentose phosphate pathway (OPPP) revealed comparable effects, we investigated 
      whether GPT1 may dually localize to plastids and peroxisomes. In reporter 
      fusions, GPT2 localized to plastids, but GPT1 also localized to the endoplasmic 
      reticulum (ER) and around peroxisomes. GPT1 contacted two oxidoreductases and 
      also peroxins that mediate import of peroxisomal membrane proteins from the ER, 
      hinting at dual localization. Reconstitution in yeast (Saccharomyces cerevisiae) 
      proteoliposomes revealed that GPT1 preferentially exchanges G6P for 
      ribulose-5-phosphate (Ru5P). Complementation analyses of heterozygous +/gpt1 
      plants demonstrated that GPT2 is unable to compensate for GPT1 in plastids, 
      whereas GPT1 without the transit peptide (enforcing ER/peroxisomal localization) 
      increased gpt1 transmission significantly. Because OPPP activity in peroxisomes 
      is essential for fertilization, and immunoblot analyses hinted at the presence of 
      unprocessed GPT1-specific bands, our findings suggest that GPT1 is indispensable 
      in both plastids and peroxisomes. Together with its G6P-Ru5P exchange preference, 
      GPT1 appears to play a role distinct from that of GPT2 due to dual targeting.
CI  - (c) 2020 American Society of Plant Biologists. All rights reserved.
FAU - Baune, Marie-Christin
AU  - Baune MC
AUID- ORCID: 0000-0003-0869-1320
AD  - Institut fur Biologie und Biotechnologie der Pflanzen, Westfalische 
      Wilhelms-Universitat Munster, 48149 Munster, Germany.
FAU - Lansing, Hannes
AU  - Lansing H
AUID- ORCID: 0000-0001-5716-2877
AD  - Institut fur Biologie und Biotechnologie der Pflanzen, Westfalische 
      Wilhelms-Universitat Munster, 48149 Munster, Germany.
FAU - Fischer, Kerstin
AU  - Fischer K
AUID- ORCID: 0000-0002-2200-8420
AD  - Institut fur Biologie und Biotechnologie der Pflanzen, Westfalische 
      Wilhelms-Universitat Munster, 48149 Munster, Germany.
FAU - Meyer, Tanja
AU  - Meyer T
AUID- ORCID: 0000-0002-5541-0563
AD  - Institut fur Biologie und Biotechnologie der Pflanzen, Westfalische 
      Wilhelms-Universitat Munster, 48149 Munster, Germany.
FAU - Charton, Lennart
AU  - Charton L
AUID- ORCID: 0000-0001-6289-4431
AD  - Biochemie der Pflanzen, Heinrich-Heine-Universitat Dusseldorf, 40225 Dusseldorf, 
      Germany.
FAU - Linka, Nicole
AU  - Linka N
AUID- ORCID: 0000-0002-1400-717X
AD  - Biochemie der Pflanzen, Heinrich-Heine-Universitat Dusseldorf, 40225 Dusseldorf, 
      Germany.
FAU - von Schaewen, Antje
AU  - von Schaewen A
AUID- ORCID: 0000-0003-0832-108X
AD  - Institut fur Biologie und Biotechnologie der Pflanzen, Westfalische 
      Wilhelms-Universitat Munster, 48149 Munster, Germany schaewen@uni-muenster.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200228
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (AT5G54800 protein, Arabidopsis)
RN  - 0 (Amino Acids)
RN  - 0 (Antiporters)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Ribulosephosphates)
RN  - 0 (glucose 6-phosphate(transporter))
RN  - 4151-19-3 (ribulose 5-phosphate)
RN  - 56-73-5 (Glucose-6-Phosphate)
SB  - IM
MH  - Alleles
MH  - Amino Acids/metabolism
MH  - Antiporters/chemistry/*metabolism
MH  - Arabidopsis/*metabolism
MH  - Arabidopsis Proteins/chemistry/*metabolism
MH  - Cytosol/metabolism
MH  - Endoplasmic Reticulum/*metabolism
MH  - Fertilization
MH  - Glucose-6-Phosphate/metabolism
MH  - Models, Biological
MH  - Monosaccharide Transport Proteins/chemistry/*metabolism
MH  - Ovule/metabolism
MH  - Oxidation-Reduction
MH  - Peroxisomes/*metabolism
MH  - Phylogeny
MH  - Plastids/*metabolism
MH  - Protein Domains
MH  - Protein Multimerization
MH  - Protein Transport
MH  - Ribulosephosphates/metabolism
MH  - Seeds/metabolism
MH  - Stress, Physiological
PMC - PMC7203913
EDAT- 2020/03/01 06:00
MHDA- 2021/01/29 06:00
PMCR- 2020/02/28
CRDT- 2020/03/01 06:00
PHST- 2019/12/11 00:00 [received]
PHST- 2020/01/28 00:00 [revised]
PHST- 2020/02/28 00:00 [accepted]
PHST- 2020/03/01 06:00 [pubmed]
PHST- 2021/01/29 06:00 [medline]
PHST- 2020/03/01 06:00 [entrez]
PHST- 2020/02/28 00:00 [pmc-release]
AID - tpc.19.00959 [pii]
AID - 201900959DR1 [pii]
AID - 10.1105/tpc.19.00959 [doi]
PST - ppublish
SO  - Plant Cell. 2020 May;32(5):1703-1726. doi: 10.1105/tpc.19.00959. Epub 2020 Feb 
      28.