PMID- 32111932
OWN - NLM
STAT- MEDLINE
DCOM- 20201110
LR  - 20231113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Feb 28
TI  - In situ identification of polyhydroxyalkanoate (PHA)-accumulating microorganisms 
      in mixed microbial cultures under feast/famine conditions.
PG  - 3752
LID - 10.1038/s41598-020-60727-7 [doi]
LID - 3752
AB  - The accumulation of plastic waste in the environment has become a serious 
      environmental problem worldwide. Biodegradable plastics, such as 
      polyhydroxyalkanoate (PHA), could serve as green alternatives to petroleum-based 
      plastics. In this study, a mixed microbial culture was enriched under 
      feast/famine conditions using a sequencing batch reactor (SBR) with acetate as a 
      carbon source. The enrichment could accumulate a maximum PHA concentration of 
      32.3% gPHA/g mixed liquor suspended solids (MLSS) in the 12(th) cycle of SBR 
      operation. The microbial community in this sludge sample was analyzed using 16 S 
      rRNA gene amplicon sequencing (MiSeq). The results showed the dominance of 
      Proteobacteria, represented by Alphaproteobacteria (13.26% of total sequences), 
      Betaproteobacteria (51.37% of total sequences), and Gammaproteobacteria (23.44% 
      of total sequences). Thauera (Betaproteobacteria) had the highest relative 
      abundance, accounting for 48.88% of the total sequences. PHA-accumulating 
      microorganisms in the enrichment were detected using fluorescence in situ 
      hybridization (FISH) and a fluorescent dye, Nile blue A. Alphaproteobacteria and 
      Betaproteobacteria were capable of accumulating PHA, while no Gammaproteobacteria 
      were detected. Thauera spp. from Betaproteobacteria constituted 80.3% of the 
      total PHA accumulating cells.
FAU - Sruamsiri, Donhatai
AU  - Sruamsiri D
AD  - Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn 
      University, Bangkok, Thailand.
FAU - Thayanukul, Parinda
AU  - Thayanukul P
AD  - Department of Environmental Engineering, Faculty of Engineering, King Mongkut's 
      University of Technology Thonburi, Bangkok, Thailand.
AD  - Center of Excellence on Hazardous Substance Management, Chulalongkorn University, 
      Bangkok, Thailand.
AD  - Department of Biology, Faculty of Science, Mahidol University, Bangkok, 10400, 
      Thailand.
FAU - Suwannasilp, Benjaporn Boonchayaanant
AU  - Suwannasilp BB
AD  - Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn 
      University, Bangkok, Thailand. benjaporn.bo@chula.ac.th.
AD  - Center of Excellence on Hazardous Substance Management, Chulalongkorn University, 
      Bangkok, Thailand. benjaporn.bo@chula.ac.th.
AD  - Research Network of NANOTEC-CU (RNN), Bangkok, Thailand. 
      benjaporn.bo@chula.ac.th.
AD  - Biotechnology for Wastewater Engineering Research Group, Chulalongkorn 
      University, Bangkok, Thailand. benjaporn.bo@chula.ac.th.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200228
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Polyhydroxyalkanoates)
SB  - IM
MH  - Bacteria/classification/*growth & development
MH  - *Bioreactors
MH  - Polyhydroxyalkanoates/*metabolism
PMC - PMC7048717
COIS- The authors declare no competing interests.
EDAT- 2020/03/01 06:00
MHDA- 2020/11/11 06:00
PMCR- 2020/02/28
CRDT- 2020/03/01 06:00
PHST- 2019/12/05 00:00 [received]
PHST- 2020/02/14 00:00 [accepted]
PHST- 2020/03/01 06:00 [entrez]
PHST- 2020/03/01 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
PHST- 2020/02/28 00:00 [pmc-release]
AID - 10.1038/s41598-020-60727-7 [pii]
AID - 60727 [pii]
AID - 10.1038/s41598-020-60727-7 [doi]
PST - epublish
SO  - Sci Rep. 2020 Feb 28;10(1):3752. doi: 10.1038/s41598-020-60727-7.