PMID- 32116301
OWN - NLM
STAT- MEDLINE
DCOM- 20200911
LR  - 20200911
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 156
DP  - 2020 Feb 16
TI  - Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide 
      Nanoprobes for Extrapulmonary Tuberculosis Detection.
LID - 10.3791/58227 [doi]
AB  - A molecular imaging probe comprising superparamagnetic iron oxide (SPIO) 
      nanoparticles and Mycobacterium tuberculosis surface antibody (MtbsAb) was 
      synthesized to enhance imaging sensitivity for extrapulmonary tuberculosis (ETB). 
      An SPIO nanoprobe was synthesized and conjugated with MtbsAb. The purified 
      SPIO-MtbsAb nanoprobe was characterized using TEM and NMR. To determine the 
      targeting ability of the probe, SPIO-MtbsAb nanoprobes were incubated with Mtb 
      for in vitro imaging assays and injected into Mtb-inoculated mice for in vivo 
      investigation with magnetic resonance (MR). The contrast enhancement reduction on 
      magnetic resonance imaging (MRI) of Mtb and THP1 cells showed proportional to the 
      SPIO-MtbsAb nanoprobe concentration. After 30 min of intravenous SPIO-MtbsAb 
      nanoprobe injection into Mtb-infected mice, the signal intensity of the 
      granulomatous site was enhanced by 14-fold in the T2-weighted MR images compared 
      with that in mice receiving PBS injection. The MtbsAb nanoprobes can be used as a 
      novel modality for ETB detection.
FAU - Lee, Chun-Nin
AU  - Lee CN
AD  - Department of Pulmonary and Critical Care Medicine, Taipei Medical 
      University-Shuang Ho Hospital.
FAU - Chiu, Li-Hsuan
AU  - Chiu LH
AD  - Department of Research and Department of Dentistry, Taipei Medical University / 
      Shuang-Ho Hospital; McLean Imaging Center, McLean Hospital/Harvard Medical 
      School.
FAU - Fang, Chia-Lang
AU  - Fang CL
AD  - Department of Pathology, Taipei Medical University.
FAU - Yeh, Shauh-Der
AU  - Yeh SD
AD  - Department of Urology, School of Medicine, College of Medicine, Taipei Medical 
      University.
FAU - Zuo, Chun S
AU  - Zuo CS
AD  - McLean Imaging Center, McLean Hospital/Harvard Medical School.
FAU - Chen, Shih-Ching
AU  - Chen SC
AD  - Department of Physical Medicine and Rehabilitation, School of Medicine, College 
      of Medicine, Taipei Medical University.
FAU - Kuo, Li-Kuo
AU  - Kuo LK
AD  - Department of Medicine, Mackay Medical College.
FAU - Wang, Yun-Ming
AU  - Wang YM
AD  - Department of Biological Science and Technology, National Chiao Tung University; 
      ymwang@mail.nctu.edu.tw.
FAU - Lai, Wen-Fu Thomas
AU  - Lai WT
AD  - Department of Research and Department of Dentistry, Taipei Medical University / 
      Shuang-Ho Hospital; McLean Imaging Center, McLean Hospital/Harvard Medical 
      School; Graduate Institute of Clinical Medicine, Taipei Medical University; 
      laitw@tmu.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20200216
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Dextrans)
RN  - 0 (Ferric Compounds)
RN  - 0 (Magnetite Nanoparticles)
RN  - 1K09F3G675 (ferric oxide)
RN  - G6N3J05W84 (ferumoxides)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/immunology
MH  - Dextrans/*chemical synthesis
MH  - Ferric Compounds
MH  - Humans
MH  - Injections, Intravenous
MH  - Magnetic Resonance Imaging
MH  - Magnetite Nanoparticles/*chemistry/ultrastructure
MH  - Mice, Inbred C57BL
MH  - Mycobacterium tuberculosis/immunology
MH  - Particle Size
MH  - THP-1 Cells
MH  - Tuberculosis/*diagnosis/immunology/microbiology/pathology
EDAT- 2020/03/03 06:00
MHDA- 2020/09/12 06:00
CRDT- 2020/03/03 06:00
PHST- 2020/03/03 06:00 [entrez]
PHST- 2020/03/03 06:00 [pubmed]
PHST- 2020/09/12 06:00 [medline]
AID - 10.3791/58227 [doi]
PST - epublish
SO  - J Vis Exp. 2020 Feb 16;(156). doi: 10.3791/58227.