PMID- 32124948
OWN - NLM
STAT- MEDLINE
DCOM- 20201130
LR  - 20201130
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 45
IP  - 4
DP  - 2020 Apr
TI  - Antitumor effects and mechanisms of pyropheophorbide‑alpha methyl ester‑mediated 
      photodynamic therapy on the human osteosarcoma cell line MG‑63.
PG  - 971-982
LID - 10.3892/ijmm.2020.4494 [doi]
AB  - Photodynamic therapy (PDT) is a promising treatment for osteosarcoma, and 
      pyropheophorbide‑alpha methyl ester (MPPa) is a second‑generation photosensitizer for 
      tumor treatment. The present study aimed to determine the efficacy and possible 
      mechanisms of MPPa‑PDT in the treatment of osteosarcoma MG‑63 cells. Flow 
      cytometry and western blotting were used to detect cell cycle‑related indicators 
      Cyclin D1, Cyclin E, Cyclin A and Cyclin B1. Cell migration and invasion 
      abilities were detected using wound‑healing and Transwell chamber assays. 
      Cellular endoplasmic reticulum stress (ERS), autophagy and apoptosis‑related 
      indicators were detected by flow cytometry and western blotting. The results 
      demonstrated that MPPa‑PDT blocked the MG‑63 cell cycle and inhibited cell 
      migration and invasion. Additionally, MPPa‑PDT inhibited the activation of the 
      Akt/mammalian target of rapamycin (mTOR) pathway. MG‑63 cells underwent 
      ERS‑induced apoptosis following MPPa‑PDT treatment. Pretreatment with the mTOR 
      phosphorylation inhibitor rapamycin affected the autophagy of MPPa‑PDT‑induced 
      osteosarcoma MG‑63 cells and enhanced apoptosis through targeting mTOR.
FAU - Chen, Yanyang
AU  - Chen Y
AD  - Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Yin, Hang
AU  - Yin H
AD  - Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Tao, Yong
AU  - Tao Y
AD  - Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Zhong, Shenxi
AU  - Zhong S
AD  - Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Yu, Haoyang
AU  - Yu H
AD  - Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Li, Jianxiao
AU  - Li J
AD  - Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Bai, Zhibiao
AU  - Bai Z
AD  - Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Ou, Yunsheng
AU  - Ou Y
AD  - Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200210
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Photosensitizing Agents)
RN  - 0 (Porphyrins)
RN  - 0 (pyropheophorbide-a methylester)
SB  - IM
MH  - Bone Neoplasms/*drug therapy/metabolism/pathology
MH  - Cell Line, Tumor
MH  - Humans
MH  - Neoplasm Proteins/metabolism
MH  - Osteosarcoma/*drug therapy/metabolism/pathology
MH  - *Photochemotherapy
MH  - Photosensitizing Agents/*pharmacology
MH  - Porphyrins/*pharmacology
MH  - Signal Transduction/drug effects
PMC - PMC7053850
EDAT- 2020/03/04 06:00
MHDA- 2020/12/01 06:00
PMCR- 2020/02/10
CRDT- 2020/03/04 06:00
PHST- 2018/08/10 00:00 [received]
PHST- 2019/11/20 00:00 [accepted]
PHST- 2020/03/04 06:00 [pubmed]
PHST- 2020/12/01 06:00 [medline]
PHST- 2020/03/04 06:00 [entrez]
PHST- 2020/02/10 00:00 [pmc-release]
AID - ijmm-45-04-0971 [pii]
AID - 10.3892/ijmm.2020.4494 [doi]
PST - ppublish
SO  - Int J Mol Med. 2020 Apr;45(4):971-982. doi: 10.3892/ijmm.2020.4494. Epub 2020 Feb 
      10.