PMID- 32126420
OWN - NLM
STAT- MEDLINE
DCOM- 20210326
LR  - 20210326
IS  - 1873-3336 (Electronic)
IS  - 0304-3894 (Linking)
VI  - 393
DP  - 2020 Jul 5
TI  - Inactivation of airborne porcine reproductive and respiratory syndrome virus 
      (PRRSv) by a packed bed dielectric barrier discharge non-thermal plasma.
PG  - 122266
LID - S0304-3894(20)30254-5 [pii]
LID - 10.1016/j.jhazmat.2020.122266 [doi]
AB  - Porcine reproductive and respiratory syndrome virus (PRRSv) is one of the most 
      significant airborne viruses impacting the pork industry in the US. Non-thermal 
      plasmas (NTPs) are electrical discharges comprised of reactive radicals and 
      excited species that inactivate viruses and bacteria. Our previous experiments 
      using a packed bed NTP reactor demonstrated effective inactivation of 
      bacteriophage MS2 as a function of applied voltage and power. The present study 
      examined the effectiveness of the same reactor in inactivating aerosolized PRRSv. 
      A PRRSv solution containing  approximately 10(5) TCID(50)/ml of PRRSv VR2332 strain was 
      aerosolized at 3 ml/min by an air-jet nebulizer and introduced into 5 or 12 cfm 
      air flow followed by NTP exposure in the reactor. Twin impingers upstream and 
      downstream of the reactor collected samples of the virus-laden air flow for 
      subsequent TCID(50) assay and qPCR analyses. An optical particle sizer measured 
      upstream and downstream aerosol size distributions, giving estimates of aerosol 
      filtration by the reactor. The results showed that PRRSv was inactivated to a 
      similar degree as MS2 at the same conditions, with the maximum 1.3-log 
      inactivation of PRRSv achieved at 20 kV and 12 cfm air flow rate. The results 
      demonstrate the potential of properly optimized NTPs in controlling PRRSv 
      transmission.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Xia, T
AU  - Xia T
AD  - Civil and Environmental Engineering, University of Michigan, Ann Arbor, MI, 
      United States. Electronic address: xiatian@umich.edu.
FAU - Yang, M
AU  - Yang M
AD  - Veterinary Population Medicine, University of Minnesota, St. Paul, MN, United 
      States.
FAU - Marabella, I
AU  - Marabella I
AD  - Mechanical Engineering, University of Minnesota, Minneapolis, MN, United States.
FAU - Lee, E M
AU  - Lee EM
AD  - Mechanical, Materials and Aerospace Engineering, Illinois Institute of 
      Technology, Chicago, IL, United States.
FAU - Olson, B
AU  - Olson B
AD  - Mechanical Engineering, University of Minnesota, Minneapolis, MN, United States.
FAU - Zarling, D
AU  - Zarling D
AD  - Mechanical Engineering, University of Minnesota, Minneapolis, MN, United States.
FAU - Torremorell, M
AU  - Torremorell M
AD  - Veterinary Population Medicine, University of Minnesota, St. Paul, MN, United 
      States.
FAU - Clack, H L
AU  - Clack HL
AD  - Civil and Environmental Engineering, University of Michigan, Ann Arbor, MI, 
      United States.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200210
PL  - Netherlands
TA  - J Hazard Mater
JT  - Journal of hazardous materials
JID - 9422688
RN  - 0 (Aerosols)
RN  - 0 (Plasma Gases)
SB  - IM
MH  - Aerosols/chemistry
MH  - Air Microbiology
MH  - Levivirus/drug effects
MH  - Plasma Gases/*pharmacology
MH  - Porcine respiratory and reproductive syndrome virus/*drug effects
OTO - NOTNLM
OT  - Airstream inactivation
OT  - Non-thermal plasma
OT  - PRRSv
OT  - TCID(50)
OT  - qPCR
COIS- Declaration of Competing Interest EML and HLC are affiliated with Taza Aya LLC; 
      HLC is co-founder and CTO. TX, MY, IM, BO, DZ, and MT all declare no competing 
      financial interest.
EDAT- 2020/03/04 06:00
MHDA- 2021/03/27 06:00
CRDT- 2020/03/04 06:00
PHST- 2019/10/13 00:00 [received]
PHST- 2020/02/04 00:00 [revised]
PHST- 2020/02/08 00:00 [accepted]
PHST- 2020/03/04 06:00 [pubmed]
PHST- 2021/03/27 06:00 [medline]
PHST- 2020/03/04 06:00 [entrez]
AID - S0304-3894(20)30254-5 [pii]
AID - 10.1016/j.jhazmat.2020.122266 [doi]
PST - ppublish
SO  - J Hazard Mater. 2020 Jul 5;393:122266. doi: 10.1016/j.jhazmat.2020.122266. Epub 
      2020 Feb 10.