PMID- 32129184 OWN - NLM STAT- MEDLINE DCOM- 20200306 LR - 20221207 IS - 1477-8920 (Print) IS - 1477-8920 (Linking) VI - 18 IP - 1 DP - 2020 Feb TI - Environmental monitoring of Aichi virus and human bocavirus in samples from wastewater treatment plant, drain, and River Nile in Egypt. PG - 30-37 LID - 10.2166/wh.2019.075 [doi] AB - Wastewater plays a major role in water pollution causing transmission of several viral pathogens, including Aichi virus (AiV) and human bocavirus (HBoV), associated with gastrointestinal illness in humans. In this study, we investigated the presence of AiV and HBoV in aquatic, sludge, sediment matrices collected from Abu-Rawash wastewater treatment plant (WWTP), El-Rahawy drain, Rosetta branch of the River Nile in Egypt by conventional polymerase chain reaction (PCR). AiV RNA was detected in 16.6% (2/12), 8.3% (1/12), 8.3% (1/12), 22% (16/72), 12.5% (3/24), 4% (1/24), and 0/24 (0%) of untreated raw sewage, treated sewage, sewage sludge, drainage water, drain sediment, river water, and river sediment, respectively. On the other hand, HBoV DNA was detected in 41.6% (5/12), 25% (3/12), 16.6% (2/12), 48.6% (35/72), 29% (7/24), 3/24 (12.5%), 4% (1/24) of untreated raw sewage, treated raw sewage, sewage sludge, drainage water, drain sediment, river water, and river sediment, respectively. This study provides data on the presence of these viruses in various types of water samples that are valuable to environmental risk assessment. In addition, the current study demonstrates the importance of environmental monitoring as an additional tool to investigate the epidemiology of AiV and HBoV circulating in a given community. FAU - Shaheen, Mohamed N F AU - Shaheen MNF AD - Environmental Virology Laboratory, Water Pollution Research Department, Environmental Research Division, National Research Centre, Al-Buhouth Street, 12622 Dokki, Giza, Egypt E-mail: m_nrc2007@yahoo.com. FAU - Abd El-Daim, Sahar E AU - Abd El-Daim SE AD - Environmental Virology Laboratory, Water Pollution Research Department, Environmental Research Division, National Research Centre, Al-Buhouth Street, 12622 Dokki, Giza, Egypt E-mail: m_nrc2007@yahoo.com. FAU - Ahmed, Nehal I AU - Ahmed NI AD - Environmental Virology Laboratory, Water Pollution Research Department, Environmental Research Division, National Research Centre, Al-Buhouth Street, 12622 Dokki, Giza, Egypt E-mail: m_nrc2007@yahoo.com. FAU - Elmahdy, Elmahdy M AU - Elmahdy EM AD - Environmental Virology Laboratory, Water Pollution Research Department, Environmental Research Division, National Research Centre, Al-Buhouth Street, 12622 Dokki, Giza, Egypt E-mail: m_nrc2007@yahoo.com. LA - eng PT - Journal Article PL - England TA - J Water Health JT - Journal of water and health JID - 101185420 RN - 0 (Sewage) RN - 0 (Waste Water) SB - IM MH - Egypt MH - *Environmental Monitoring MH - *Human bocavirus MH - Humans MH - *Kobuvirus MH - Rivers/*virology MH - Sewage MH - *Waste Disposal, Fluid MH - Wastewater/*virology EDAT- 2020/03/05 06:00 MHDA- 2020/03/07 06:00 CRDT- 2020/03/05 06:00 PHST- 2020/03/05 06:00 [entrez] PHST- 2020/03/05 06:00 [pubmed] PHST- 2020/03/07 06:00 [medline] AID - 10.2166/wh.2019.075 [doi] PST - ppublish SO - J Water Health. 2020 Feb;18(1):30-37. doi: 10.2166/wh.2019.075.