PMID- 32130380
OWN - NLM
STAT- MEDLINE
DCOM- 20200415
LR  - 20200415
IS  - 1806-9282 (Electronic)
IS  - 0104-4230 (Linking)
VI  - 66
IP  - 1
DP  - 2020
TI  - ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic 
      indicator of colorectal cancer.
PG  - 42-47
LID - S0104-42302020000100042 [pii]
LID - 10.1590/1806-9282.66.1.42 [doi]
AB  - OBJECTIVE: ADAMTS4 is a member of the ADAMTS4 family, which secretes proteinases. 
      The mechanism of tumor metastasis may be correlated to its promotion of 
      angiogenesis. It was determined whether ADAMTS4 participates in colorectal cancer 
      progression. METHODS: The expression in clinical samples and CRC cell lines was 
      investigated. Using immunohistochemistry (IHC), fluorescence in situ 
      hybridization (FISH), and RT-PCR, the expression of ADAMTS4 was determined in 
      colorectal tumors of different cancer stages and anatomic sites, and in three 
      cell lines of different aggressiveness. RESULTS: The overexpression of ADAMTS4 
      was observed in tissue samples by IHC, and this was mainly located in the 
      cytoplasm, as detected by FISH. The qRT-PCR and western blot analyses further 
      supported the clinical sample findings. CONCLUSION: The present data support the 
      notion that the overexpression of ADAMTS4 in CRC might be useful as a 
      non-invasive biomarker for detecting CRC in patients.
FAU - Shang, Xue-Qin
AU  - Shang XQ
AUID- ORCID: 0000-0002-6740-2341
AD  - . Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical 
      University, Beijing, 100038, China.
AD  - . The Second People's Hospital of Yunnan Province, Oncology Department, Kunming, 
      650021, China.
FAU - Liu, Kui-Liang
AU  - Liu KL
AUID- ORCID: 0000-0001-9972-468X
AD  - . Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical 
      University, Beijing, 100038, China.
FAU - Li, Qian
AU  - Li Q
AUID- ORCID: 0000-0001-5166-1704
AD  - . Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical 
      University, Beijing, 100038, China.
FAU - Lao, Yue-Qiong
AU  - Lao YQ
AUID- ORCID: 0000-0002-6445-965X
AD  - . Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical 
      University, Beijing, 100038, China.
FAU - Li, Nan-Shan
AU  - Li NS
AUID- ORCID: 0000-0002-2725-5906
AD  - . Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical 
      University, Beijing, 100038, China.
FAU - Wu, Jing
AU  - Wu J
AUID- ORCID: 0000-0001-7430-7003
AD  - . Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical 
      University, Beijing, 100038, China.
LA  - eng
PT  - Journal Article
DEP - 20200227
PL  - Brazil
TA  - Rev Assoc Med Bras (1992)
JT  - Revista da Associacao Medica Brasileira (1992)
JID - 9308586
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.82 (ADAMTS4 Protein)
RN  - EC 3.4.24.82 (ADAMTS4 protein, human)
SB  - IM
MH  - ADAMTS4 Protein/*analysis
MH  - Aged
MH  - Analysis of Variance
MH  - Biomarkers, Tumor
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - Colorectal Neoplasms/genetics/*pathology
MH  - Disease Progression
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - RNA, Messenger/analysis
MH  - Reference Values
MH  - Up-Regulation
EDAT- 2020/03/05 06:00
MHDA- 2020/04/16 06:00
CRDT- 2020/03/05 06:00
PHST- 2019/05/21 00:00 [received]
PHST- 2019/06/30 00:00 [accepted]
PHST- 2020/03/05 06:00 [entrez]
PHST- 2020/03/05 06:00 [pubmed]
PHST- 2020/04/16 06:00 [medline]
AID - S0104-42302020000100042 [pii]
AID - 10.1590/1806-9282.66.1.42 [doi]
PST - epublish
SO  - Rev Assoc Med Bras (1992). 2020 Feb 27;66(1):42-47. doi: 
      10.1590/1806-9282.66.1.42. eCollection 2020.