PMID- 32130677
OWN - NLM
STAT- MEDLINE
DCOM- 20200902
LR  - 20210210
IS  - 1749-0774 (Electronic)
IS  - 0914-7470 (Linking)
VI  - 33
IP  - 3
DP  - 2020 Jul
TI  - Overexpression of tissue factor pathway inhibitor 2 attenuates trophoblast 
      proliferation and invasion in preeclampsia.
PG  - 512-520
LID - 10.1007/s13577-020-00322-0 [doi]
AB  - Pre-eclampsia (PE) is a disorder of pregnancy characterized by proteinuria and 
      high blood pressure, affecting 2-8% of pregnancies worldwide. Previous studies 
      have shown that PE is closely associated with trophoblast cell dysfunction. Here, 
      we investigated the role of tissue factor pathway inhibitor-2 (TFPI-2) in 
      regulating the biological processes of trophoblast cells. The TFPI-2 levels in 
      plasma samples and placental tissues were tested by ELISA, immunohistochemistry, 
      qRT-PCR, and western blot. HTR8/Svneo cell line was used to simulate the primary 
      trophoblast cells and H/R culture was applied to mimic the oxidative stress state 
      of PE. MTT assay, Annexin V/propidium iodide (PI) apoptosis assay, and transwell 
      assay were used to determine the cell proliferation, apoptosis, and invasion. The 
      expression levels of matrix metalloproteinases (MMPs) were evaluated by western 
      blot. The expression of TFPI-2 was remarkably up-regulated in both the serum and 
      placenta of PE patients. Hypoxia/reoxygenation increased the expression of TFPI-2 
      in HTR-8/SVneo cell line. TFPI-2 promoted that cell proliferation and inhibited 
      the cell apoptosis of HTR8/SVneo cells in H/R condition. In addition, 
      downregulation of TFPI-2 increased the cell invasion and the expression of MMP2 
      and MMP9. This study reveals that TFPI-2 plays a crucial role in monitoring the 
      biological function of trophoblast cells, which might provide theoretical basis 
      and therapeutic targets for the treatment of PE.
FAU - Zheng, Lili
AU  - Zheng L
AD  - Department of Obstetrics, The Second Hospital of Hebei Medical University, No. 
      215 Heping Xi Road, Shijiazhuang, 050000, Hebei, China.
FAU - Huang, Jing
AU  - Huang J
AD  - Department of Obstetrics, The Second Hospital of Hebei Medical University, No. 
      215 Heping Xi Road, Shijiazhuang, 050000, Hebei, China.
FAU - Su, Yuan
AU  - Su Y
AD  - Department of Obstetrics, The Second Hospital of Hebei Medical University, No. 
      215 Heping Xi Road, Shijiazhuang, 050000, Hebei, China.
FAU - Wang, Fang
AU  - Wang F
AD  - Department of Obstetrics, The Second Hospital of Hebei Medical University, No. 
      215 Heping Xi Road, Shijiazhuang, 050000, Hebei, China.
FAU - Kong, Hongfang
AU  - Kong H
AD  - Department of Obstetrics, The Second Hospital of Hebei Medical University, No. 
      215 Heping Xi Road, Shijiazhuang, 050000, Hebei, China.
FAU - Xin, Hong
AU  - Xin H
AD  - Department of Obstetrics, The Second Hospital of Hebei Medical University, No. 
      215 Heping Xi Road, Shijiazhuang, 050000, Hebei, China. xh_hbyd@163.com.
LA  - eng
GR  - 17277739D/the Hebei Science and Technology Support Project/
PT  - Journal Article
DEP - 20200304
PL  - Japan
TA  - Hum Cell
JT  - Human cell
JID - 8912329
RN  - 0 (Glycoproteins)
RN  - 0 (tissue-factor-pathway inhibitor 2)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (MMP9 protein, human)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Cell Proliferation/*genetics
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression/genetics
MH  - Glycoproteins/genetics/metabolism/*physiology
MH  - Humans
MH  - Matrix Metalloproteinase 2/genetics/metabolism
MH  - Matrix Metalloproteinase 9/genetics/metabolism
MH  - Molecular Targeted Therapy
MH  - Pre-Eclampsia/genetics/*pathology/therapy
MH  - Pregnancy
MH  - Trophoblasts/*pathology
MH  - Up-Regulation/genetics
OTO - NOTNLM
OT  - Metalloproteinases (MMPs)
OT  - Pre-eclampsia (PE)
OT  - Tissue factor pathway inhibitor-2 (TFPI-2)
OT  - Trophoblast cells
EDAT- 2020/03/05 06:00
MHDA- 2020/09/04 06:00
CRDT- 2020/03/05 06:00
PHST- 2019/10/31 00:00 [received]
PHST- 2020/01/12 00:00 [accepted]
PHST- 2020/03/05 06:00 [pubmed]
PHST- 2020/09/04 06:00 [medline]
PHST- 2020/03/05 06:00 [entrez]
AID - 10.1007/s13577-020-00322-0 [pii]
AID - 10.1007/s13577-020-00322-0 [doi]
PST - ppublish
SO  - Hum Cell. 2020 Jul;33(3):512-520. doi: 10.1007/s13577-020-00322-0. Epub 2020 Mar 
      4.