PMID- 32132267
OWN - NLM
STAT- MEDLINE
DCOM- 20200309
LR  - 20200309
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 138
DP  - 2020 Jan 30
TI  - Detection of ostreid herpesvirus-1 in plankton and seawater samples at an estuary 
      scale.
PG  - 1-15
LID - 10.3354/dao03445 [doi]
AB  - Ostreid herpesvirus-1 (OsHV-1) is known to associate with particles in seawater, 
      leading to infection and disease in the Pacific oyster Crassostrea gigas. The 
      estuarine environment is highly complex and changeable, and this needs to be 
      considered when collecting environmental samples for pathogen detection. The aims 
      of this study were to (1) compare different aspects of collecting natural 
      seawater and plankton samples for detection of OsHV-1 DNA and (2) determine 
      whether detection of OsHV-1 DNA in such environmental samples has merit for 
      disease risk prediction. The results of one experiment suggest that sampling on 
      the outgoing tide may improve the detection of OsHV-1 DNA in seawater and 
      plankton tow samples (odds ratio 2.71). This statistical comparison was not 
      possible in 2 other experiments. The method (plankton tow or beta bottle) and 
      depth of collection (range: 250-1250 mm) had no effect on the likelihood of 
      detection of OsHV-1. OsHV-1 DNA was found at low concentrations in plankton tow 
      and seawater samples, and only when outbreaks of mortality associated with OsHV-1 
      were observed in nearby experimental or farmed populations of C. gigas. This 
      suggests that single point in time environmental samples of seawater or plankton 
      are not sufficient to rule out the presence of OsHV-1 in an estuary. The 
      association of OsHV-1 with particles in seawater needs to be better understood in 
      order to determine whether more selective and sensitive methods can be devised to 
      detect it, before environmental samples can be reliably used in disease risk 
      prediction.
FAU - Liu, Olivia
AU  - Liu O
AD  - School of Veterinary Science, Faculty of Science, The University of Sydney, 
      Camden, NSW 2570, Australia.
FAU - Paul-Pont, Ika
AU  - Paul-Pont I
FAU - Rubio, Ana
AU  - Rubio A
FAU - Dhand, Navneet
AU  - Dhand N
FAU - Whittington, Richard J
AU  - Whittington RJ
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Crassostrea
MH  - DNA, Viral
MH  - Estuaries
MH  - *Herpesviridae
MH  - Plankton
MH  - Seawater
OTO - NOTNLM
OT  - Crassostrea gigas
OT  - Detection
OT  - Disease
OT  - Ostreid herpesvirus-1
OT  - Plankton
OT  - Seawater
EDAT- 2020/03/07 06:00
MHDA- 2020/03/10 06:00
CRDT- 2020/03/06 06:00
PHST- 2020/03/06 06:00 [entrez]
PHST- 2020/03/07 06:00 [pubmed]
PHST- 2020/03/10 06:00 [medline]
AID - 10.3354/dao03445 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2020 Jan 30;138:1-15. doi: 10.3354/dao03445.