PMID- 32133380
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 7
DP  - 2020
TI  - Neutrophil Myeloperoxidase Index in Dogs With Babesiosis Caused by Babesia rossi.
PG  - 72
LID - 10.3389/fvets.2020.00072 [doi]
LID - 72
AB  - Babesiosis caused by the virulent tick-borne hemoprotozoan, Babesia rossi, 
      results in a marked systemic inflammatory host response in dogs. Neutrophils form 
      part of the innate immune response and contains myeloperoxidase (MPO) as the 
      predominant component of the neutrophil lysosomal protein in azurophilic 
      granules. The neutrophil myeloperoxidase index (MPXI), determined on the ADVIA 
      hematology analyzer, is a quantitative estimate of intracellular MPO content. 
      Objectives of this study were to: (a) compare MPXI in dogs with babesiosis with 
      healthy control dogs; (b) compare MPXI in dogs that died from babesiosis with 
      dogs that survived and controls; and (c) correlate the MPXI with the previously 
      determined segmented and band neutrophil count and cytokine concentrations in 
      dogs with babesiosis. Data for 140 dogs naturally infected with B. rossi and 20 
      healthy control dogs were retrospectively evaluated. Neutrophil counts and MPXI 
      were determined on an ADVIA 2120 analyzer. Cytokine concentrations [interleukin 
      (IL)-2, IL-6, IL-8, IL-10, IL-18, granulocyte-macrophage colony stimulating 
      factor (GM-CSF), and monocyte chemo-attractant protein-1 (MCP-1)] were determined 
      using a canine-specific multiplex immunoassay. The mortality rate of the 
      Babesia-infected dogs was 11% (15/140). MPXI was significantly higher in 
      Babesia-infected dogs (P = 0.033), and in Babesia-infected non-survivors (P = 
      0.011), compared with healthy control dogs. In Babesia-infected dogs a 
      significant positive correlation was found between MPXI and IL-10 (r = 0.211, P = 
      0.039) and a significant negative correlation was found between MPXI and IL-8 (r 
      = -0.350, P < 0.001). In Babesia-infected non-survivors, significant positive 
      correlations were found between MPXI and IL-2 (r = 0.616, P = 0.033), IL-6 (r = 
      0.615, P = 0.033), IL-18 (r = 0.613, P = 0.034), GM-CSF (r = 0.630, P = 0.028), 
      and MCP-1 (r = 0.713, P = 0.009). In Babesia-infected survivors, a significant 
      negative correlation was found between MPXI and IL-8 (r = -0.363, P = 0.001). 
      MPXI was correlated with pro-inflammatory cytokines in Babesia-infected dogs that 
      died. The potential of MPXI as a novel marker of inflammation and prognosis in 
      dogs infected with B. rossi, thus warrants further investigation.
CI  - Copyright (c) 2020 Celliers, Rautenbach, Hooijberg, Christopher and Goddard.
FAU - Celliers, Anri
AU  - Celliers A
AD  - Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, 
      University of Pretoria, Pretoria, South Africa.
FAU - Rautenbach, Yolandi
AU  - Rautenbach Y
AD  - Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, 
      University of Pretoria, Pretoria, South Africa.
FAU - Hooijberg, Emma
AU  - Hooijberg E
AD  - Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, 
      University of Pretoria, Pretoria, South Africa.
FAU - Christopher, Mary
AU  - Christopher M
AD  - Department of Pathology, Microbiology and Immunology, School of Veterinary 
      Medicine, University of California, Davis, Davis, CA, United States.
FAU - Goddard, Amelia
AU  - Goddard A
AD  - Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, 
      University of Pretoria, Pretoria, South Africa.
LA  - eng
PT  - Journal Article
DEP - 20200218
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC7040022
OTO - NOTNLM
OT  - ADVIA 2120
OT  - Babesia
OT  - MPO
OT  - MPXI
OT  - cytokines
OT  - dog
OT  - interleukin
OT  - neutrophil
EDAT- 2020/03/07 06:00
MHDA- 2020/03/07 06:01
PMCR- 2020/01/01
CRDT- 2020/03/06 06:00
PHST- 2019/11/24 00:00 [received]
PHST- 2020/01/28 00:00 [accepted]
PHST- 2020/03/06 06:00 [entrez]
PHST- 2020/03/07 06:00 [pubmed]
PHST- 2020/03/07 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2020.00072 [doi]
PST - epublish
SO  - Front Vet Sci. 2020 Feb 18;7:72. doi: 10.3389/fvets.2020.00072. eCollection 2020.