PMID- 32135314
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210724
IS  - 1873-1597 (Electronic)
IS  - 1572-1000 (Print)
IS  - 1572-1000 (Linking)
VI  - 30
DP  - 2020 Jun
TI  - Multispectral autofluorescence dermoscope for skin lesion assessment.
PG  - 101704
LID - S1572-1000(20)30057-0 [pii]
LID - 10.1016/j.pdpdt.2020.101704 [doi]
AB  - Basal cell carcinoma (BCC) is the most common type of skin cancer. Diagnosis and 
      edge assessment of BCC lesions are based on clinical and dermoscopy evaluation, 
      which are strongly dependent on the expertise and training of the physician. 
      There is a high rate of underdiagnosis because BCC is frequently confused with 
      certain common benign lesions and is often indistinguishable from the surrounding 
      healthy tissue. In the present study, a multispectral fluorescence lifetime 
      imaging (FLIm) dermoscopy system, designed for imaging and analyzing the 
      autofluorescence emission of skin tissue, was used to image thirty-eight patients 
      with diagnosed nodular BCC (nBCC) lesions, using clinically acceptable levels of 
      excitation light exposure. With this system, skin autofluorescence was imaged 
      simultaneously using three emission bands: 390 +/- 20 nm, 452 +/- 22 nm, and >496 nm, 
      preferentially targeting collagen, NADH, and FAD autofluorescence, respectively. 
      Statistical classifiers based on FLIm features developed to discriminate BCC from 
      healthy tissue showed promising performance (ROC area-under-the-curve of 0.82). 
      This study demonstrates the feasibility of clinically performing multispectral 
      endogenous FLIm dermoscopy providing baseline results indicating the potential of 
      this technology as an image-guided tool to improve the delineation of nBCC during 
      surgical lesion resection.
CI  - Copyright (c) 2020. Published by Elsevier B.V.
FAU - Romano, Renan Arnon
AU  - Romano RA
AD  - Sao Carlos Institute of Physics, University of Sao Paulo, P.O. Box 369, 
      13560-970, Sao Carlos, SP, Brazil. Electronic address: renan.romano@usp.br.
FAU - Teixeira Rosa, Ramon Gabriel
AU  - Teixeira Rosa RG
AD  - Sao Carlos Institute of Physics, University of Sao Paulo, P.O. Box 369, 
      13560-970, Sao Carlos, SP, Brazil.
FAU - Salvio, Ana Gabriela
AU  - Salvio AG
AD  - Skin Department of Amaral Carvalho Hospital, Jau, SP, Brazil.
FAU - Jo, Javier A
AU  - Jo JA
AD  - School of Electrical and Computer Engineering, University of Oklahoma, Norman, 
      OK, USA.
FAU - Kurachi, Cristina
AU  - Kurachi C
AD  - Sao Carlos Institute of Physics, University of Sao Paulo, P.O. Box 369, 
      13560-970, Sao Carlos, SP, Brazil.
LA  - eng
GR  - R01 CA218739/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20200302
PL  - Netherlands
TA  - Photodiagnosis Photodyn Ther
JT  - Photodiagnosis and photodynamic therapy
JID - 101226123
RN  - 0 (Photosensitizing Agents)
SB  - IM
MH  - *Carcinoma, Basal Cell/diagnostic imaging
MH  - Humans
MH  - Optical Imaging
MH  - *Photochemotherapy/methods
MH  - Photosensitizing Agents
MH  - *Skin Neoplasms/diagnostic imaging
PMC - PMC8299719
MID - NIHMS1612199
OTO - NOTNLM
OT  - BCC
OT  - Fluorescence lifetime imaging
OT  - In vivo
OT  - Label-free fluorescence
OT  - Skin cancer
EDAT- 2020/03/07 06:00
MHDA- 2021/05/15 06:00
PMCR- 2021/07/23
CRDT- 2020/03/06 06:00
PHST- 2019/12/04 00:00 [received]
PHST- 2020/02/07 00:00 [revised]
PHST- 2020/02/28 00:00 [accepted]
PHST- 2020/03/07 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/03/06 06:00 [entrez]
PHST- 2021/07/23 00:00 [pmc-release]
AID - S1572-1000(20)30057-0 [pii]
AID - 10.1016/j.pdpdt.2020.101704 [doi]
PST - ppublish
SO  - Photodiagnosis Photodyn Ther. 2020 Jun;30:101704. doi: 
      10.1016/j.pdpdt.2020.101704. Epub 2020 Mar 2.