PMID- 32135632
OWN - NLM
STAT- MEDLINE
DCOM- 20200310
LR  - 20200717
IS  - 0253-2727 (Print)
IS  - 2707-9740 (Electronic)
IS  - 0253-2727 (Linking)
VI  - 41
IP  - 2
DP  - 2020 Feb 14
TI  - [Chromosomal aberrations detection in chronic lymphocytic leukemia by 
      conventional cytogenetics using DSP30 and IL-2].
PG  - 143-148
LID - 10.3760/cma.j.issn.0253-2727.2020.02.011 [doi]
AB  - Objective: To study the value of unmethylated cytosine guanine dinucleotide 
      oligodeoxynucleotide (DSP30) and IL-2 in the conventional cytogenetic (CA) 
      detection of the chromosomal aberrations in chronic lymphocytic leukemia (CLL) . 
      Methods: Bone marrow or peripheral blood cells of CLL patients were cultured with 
      DSP30 plus IL-2 for 72 h, following which R-banding analysis was conducted. 
      Fluorescence in situ hybridization (FISH) was performed in 85 patients. CA 
      results were compared with data obtained by FISH. Results: Among 89 CLL patients, 
      the success rate of chromosome analysis was 94.38% (84/89) . Clonal aberrations 
      were detected in 51 patients (51/84, 60.71%) . Of them, 27 (27/51, 52.94%) were 
      complex karyotype. Among 85 CLL patients tested by FISH, chromosomal 
      abnormalities were detected in 74 (74/85, 87.06%) patients, of which 2 (2/74) 
      patients were complex karyotypes, accounting for 2.70%. Of the 85 CLL patients 
      examined by FISH, 50 had abnormal karyotype analysis, 30 had normal karyotype, 5 
      failed to have chromosome analysis. Among them, 25 cases showed clonal 
      aberrations by FISH assay but normal by CA, and 4 cases were normal by FISH but 
      displayed aberrations in chromosome analysis, and totally 78 (91.76%) cases with 
      abnormality detected by the combination of the two methods. The frequency of 13q- 
      abnormality detected by FISH was significantly higher than that by CA analysis 
      (69.41%vs 16.67%, P<0.001) , while the frequency of 11q-,+12 and 17p- detected by 
      two methods showed no significant difference (P>0.05) . The detection rate of 
      complex abnormalities in conventional karyotype analysis was higher than that in 
      FISH (50.98%vs 2.70%) . In addition, 11 low-risk and 9 intermediate-risk patients 
      according to FISH results showed complex karyotype by cytogenetics, and were 
      classified into high-risk cytogenetic subgroup. Conclusion: DSP30 and IL-2 are 
      effective in improving the detection rate of CA in CLL patients (60.71%) and CA 
      is more effective to detect complex karyotype. However, FISH had a higher overall 
      abnormality detection rate (87.06%) than CA, especially for 13q-. The combination 
      of CA and FISH not only enhanced the detection rate of clonal aberrations to 
      91.76%, but also provided more precise prognosis stratification for CLL patients, 
      thus to provide more information for clinical implication.
FAU - Liu, H F
AU  - Liu HF
AD  - Department of Hematology, The First Affiliated Hospital of Soochow University, 
      Suzhou 215006, China.
FAU - Huang, H W
AU  - Huang HW
FAU - Bai, S X
AU  - Bai SX
FAU - Gong, Y L
AU  - Gong YL
FAU - Wu, C X
AU  - Wu CX
FAU - Jin, Z M
AU  - Jin ZM
FAU - Wang, Y Y
AU  - Wang YY
FAU - Yang, Q
AU  - Yang Q
FAU - Zhang, J
AU  - Zhang J
FAU - Qiu, H Y
AU  - Qiu HY
FAU - Chen, S N
AU  - Chen SN
FAU - Pan, J L
AU  - Pan JL
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (Interleukin-2)
SB  - IM
MH  - Chromosome Aberrations
MH  - Cytogenetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interleukin-2
MH  - *Leukemia, Lymphocytic, Chronic, B-Cell
PMC - PMC7357944
OTO - NOTNLM
OT  - Cytogenetics
OT  - DSP30
OT  - FISH
OT  - IL-2
OT  - Leukemia, lymphocytic, chronic
EDAT- 2020/03/07 06:00
MHDA- 2020/03/11 06:00
PMCR- 2020/02/01
CRDT- 2020/03/06 06:00
PHST- 2020/03/06 06:00 [entrez]
PHST- 2020/03/07 06:00 [pubmed]
PHST- 2020/03/11 06:00 [medline]
PHST- 2020/02/01 00:00 [pmc-release]
AID - cjh-41-02-143 [pii]
AID - 10.3760/cma.j.issn.0253-2727.2020.02.011 [doi]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2020 Feb 14;41(2):143-148. doi: 
      10.3760/cma.j.issn.0253-2727.2020.02.011.