PMID- 321363
OWN - NLM
STAT- MEDLINE
DCOM- 19770527
LR  - 20190816
IS  - 0020-5915 (Print)
IS  - 0020-5915 (Linking)
VI  - 53
IP  - 2
DP  - 1977
TI  - Isolation of dinitrophenyl-skin protein conjugates by immunoadsorbent 
      chromatography.
PG  - 123-31
AB  - Immunoadsorbent columns charged with rabbit anti-dinitrophenyl (DNP) antibody 
      were used to isolate and concentrate DNP-protein conjugates from the 105,000 g 
      supernate of extracts prepared from guinea pig epidermis previously painted with 
      dinitrofluorobenzene (DNFB). Conjugates isolated in this manner elicited positive 
      delayed hypersensitivity skin tests when injected intradermally into 
      DNFB-sensitized animals. Dinitrophenylated epidermal protein conjugates prepared 
      in vitro were capable of specifically inhibiting the migration of peritoneal 
      exudate cells obtained from DNFB-sensitized guinea pigs. These results 
      demonstrate the application of immunoadsorbent chromatography and the migration 
      inhibition assay to the further definition of the epitopes involved in contact 
      sensitivity.
FAU - Lewis, F A
AU  - Lewis FA
FAU - Heise, E R
AU  - Heise ER
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Int Arch Allergy Appl Immunol
JT  - International archives of allergy and applied immunology
JID - 0404561
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Nitrobenzenes)
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - Cell Migration Inhibition
MH  - Dermatitis, Contact/immunology
MH  - Dinitrobenzenes/*immunology/isolation & purification
MH  - Female
MH  - Guinea Pigs
MH  - Hypersensitivity, Delayed/*immunology
MH  - Immunosorbent Techniques
MH  - Macrophages/immunology
MH  - Nitrobenzenes/*immunology
MH  - Proteins/*immunology/isolation & purification
MH  - Skin/*immunology
MH  - Skin Tests
EDAT- 1977/01/01 00:00
MHDA- 1977/01/01 00:01
CRDT- 1977/01/01 00:00
PHST- 1977/01/01 00:00 [pubmed]
PHST- 1977/01/01 00:01 [medline]
PHST- 1977/01/01 00:00 [entrez]
AID - 10.1159/000231742 [doi]
PST - ppublish
SO  - Int Arch Allergy Appl Immunol. 1977;53(2):123-31. doi: 10.1159/000231742.