PMID- 32141530
OWN - NLM
STAT- MEDLINE
DCOM- 20210301
LR  - 20210301
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 4
DP  - 2020 Feb
TI  - Synovial mesenchymal stem cells effectively alleviate osteoarthritis through 
      promoting the proliferation and differentiation of meniscus chondrocytes.
PG  - 1645-1655
LID - 20338 [pii]
LID - 10.26355/eurrev_202002_20338 [doi]
AB  - OBJECTIVE: To investigate the relationship between the meniscal defect area and 
      OA progression and explore the effect and mechanism of SMSCs cell therapy in knee 
      osteoarthritis (OA) rat model. MATERIALS AND METHODS: For animal experiments, 
      knee osteoarthritis (OA) model was constructed in Sprague Dawley (SD) rats by 
      removing the medial meniscus of the right knee. Synovial mesenchymal stem cells 
      (SMSCs) were engrafted by injecting into the right knee cavity. For in vitro 
      experiments, CCK-8 assay was performed to evaluate the proliferation and 
      differentiation of BMSCs and ATDC5 cells after co-cultured with SMSCs. qRT-PCR 
      analysis was performed to detect the expressions of chondrogenic genes in BMSCs 
      and ATDC5 cells after co-cultured with SMSCs. Western blot analysis was conducted 
      to detect the phosphorylations of c-Jun N-terminal kinase (JNK) and extracellular 
      regulated protein kinases (ERK) in MAPK signaling of BMSCs and ATDC5 cells. 
      Enzyme-linked immunosorbent assay (ELISA) was performed to detect the serum 
      levels of interleukin (IL)-1beta, IL-1beta, IL-6, IL-18 and C-reactive protein (CRP). 
      RESULTS: Results showed that meniscus damaged area is positively correlated to 
      serum inflammatory factor levels. In vitro study showed that the proliferation 
      and differentiation of BMSCs and ATDC5 cells were promoted after co-cultured with 
      SMSCs. By co-culturing with SMSCs, the MAPK signaling pathway was activated and 
      the expression of chondrogenic markers such as aggrecan (acan), SRY-related high 
      mobility group-box gene 9 (sox9) and Type II collagen a1 (col2a1), was 
      up-regulated both in BMSCs and ATDC5 cells. In vivo study showed SMSCs cell 
      therapy significantly decreased serum inflammatory factor levels and protected 
      cartilage by upregulating the expression of chondrogenic genes of meniscus 
      chondrocytes derived from OA rats. CONCLUSIONS: For the first time, we found the 
      positive correlation between meniscal defect area and OA progression and 
      demonstrated the effect and mechanism of SMSCs cell therapy in knee 
      osteoarthritis (OA) treatment.
FAU - Qiong, J
AU  - Qiong J
AD  - Department of Osteoarthritis, The First Affiliated Hospital of Hunan Traditional 
      Chinese Medicine College, Zhuzhou, Hunan, China. 04170731@163.com.
FAU - Xia, Z
AU  - Xia Z
FAU - Jing, L
AU  - Jing L
FAU - Haibin, W
AU  - Haibin W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Cytokines)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chondrocytes/*cytology/metabolism
MH  - Coculture Techniques
MH  - Cytokines/metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors/metabolism
MH  - JNK Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
MH  - Meniscus/cytology
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/metabolism
MH  - Osteoarthritis, Knee/metabolism/*therapy
MH  - Rats, Sprague-Dawley
MH  - Synovial Membrane/cytology
EDAT- 2020/03/07 06:00
MHDA- 2021/03/02 06:00
CRDT- 2020/03/07 06:00
PHST- 2020/03/07 06:00 [entrez]
PHST- 2020/03/07 06:00 [pubmed]
PHST- 2021/03/02 06:00 [medline]
AID - 20338 [pii]
AID - 10.26355/eurrev_202002_20338 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 Feb;24(4):1645-1655. doi: 
      10.26355/eurrev_202002_20338.