PMID- 32142471
OWN - NLM
STAT- MEDLINE
DCOM- 20210602
LR  - 20210602
IS  - 1437-4315 (Electronic)
IS  - 1431-6730 (Linking)
VI  - 401
IP  - 8
DP  - 2020 Jul 28
TI  - Targeting of parvulin interactors by diazirine mediated cross-linking discloses a 
      cellular role of human Par14/17 in actin polymerization.
PG  - 955-968
LID - 10.1515/hsz-2019-0423 [doi]
AB  - The peptidyl-prolyl cis/trans isomerases (PPIases) Parvulin 14 (Par14) and 
      Parvulin 17 (Par17) result from alternative transcription initiation of the PIN4 
      gene. Whereas Par14 is present in all metazoan, Par17 is only expressed in 
      Hominidae. Par14 resides mainly within the cellular nucleus, while Par17 is 
      translocated into mitochondria. Using photo-affinity labeling, cross-linking and 
      mass spectrometry (MS) we identified binding partners for both enzymes from HeLa 
      lysates and disentangled their cellular roles. Par14 is involved in biogenesis of 
      ribonucleoprotein (RNP)-complexes, RNA processing and DNA repair. Its elongated 
      isoform Par17 participates in protein transport/translocation and in cytoskeleton 
      organization. Nuclear magnetic resonance (NMR) spectroscopy reveals that Par17 
      binds to beta-actin with its N-terminal region, while both parvulins initiate actin 
      polymerization depending on their PPIase activity as monitored by fluorescence 
      spectroscopy. The knockdown (KD) of Par17 in HCT116 cells results in a defect in 
      cell motility and migration.
FAU - Goehring, Anna
AU  - Goehring A
AD  - Structural and Medicinal Biochemistry, Center for Medical Biotechnology (ZMB), 
      University of Duisburg-Essen, Universitatsstrasse 2-5, D-45117 Essen, Germany.
FAU - Michin, Irina
AU  - Michin I
AD  - Structural and Medicinal Biochemistry, Center for Medical Biotechnology (ZMB), 
      University of Duisburg-Essen, Universitatsstrasse 2-5, D-45117 Essen, Germany.
FAU - Gerdes, Tina
AU  - Gerdes T
AD  - Structural and Medicinal Biochemistry, Center for Medical Biotechnology (ZMB), 
      University of Duisburg-Essen, Universitatsstrasse 2-5, D-45117 Essen, Germany.
FAU - Schulze, Nina
AU  - Schulze N
AD  - Imaging Centre Campus Essen (ICCE), Center for Medical Biotechnology (ZMB), 
      University of Duisburg-Essen, Universitatsstrasse 2-5, D-45117 Essen, Germany.
FAU - Blueggel, Mike
AU  - Blueggel M
AD  - Structural and Medicinal Biochemistry, Center for Medical Biotechnology (ZMB), 
      University of Duisburg-Essen, Universitatsstrasse 2-5, D-45117 Essen, Germany.
FAU - Rehic, Edisa
AU  - Rehic E
AD  - Structural and Medicinal Biochemistry, Center for Medical Biotechnology (ZMB), 
      University of Duisburg-Essen, Universitatsstrasse 2-5, D-45117 Essen, Germany.
FAU - Kaschani, Farnusch
AU  - Kaschani F
AD  - Chemical Biology, Center for Medical Biotechnology (ZMB), University of 
      Duisburg-Essen, Universitatsstrasse 2-5, D-45117 Essen, Germany.
FAU - Kaiser, Markus
AU  - Kaiser M
AD  - Chemical Biology, Center for Medical Biotechnology (ZMB), University of 
      Duisburg-Essen, Universitatsstrasse 2-5, D-45117 Essen, Germany.
FAU - Bayer, Peter
AU  - Bayer P
AUID- ORCID: 0000-0003-0435-7202
AD  - Structural and Medicinal Biochemistry, Center for Medical Biotechnology (ZMB), 
      University of Duisburg-Essen, Universitatsstrasse 2-5, D-45117 Essen, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Biol Chem
JT  - Biological chemistry
JID - 9700112
RN  - 0 (Actins)
RN  - 0 (NIMA-Interacting Peptidylprolyl Isomerase)
RN  - 60A625P70P (Diazomethane)
RN  - EC 5.2.1.8 (PIN4 protein, human)
SB  - IM
MH  - Actins/*metabolism
MH  - Amino Acid Sequence
MH  - Diazomethane/pharmacology/*therapeutic use
MH  - Humans
MH  - NIMA-Interacting Peptidylprolyl Isomerase/*metabolism
MH  - Polymerization
OTO - NOTNLM
OT  - LC-MS
OT  - PPIase
OT  - mitochondria
OT  - peptidyl-prolyl cis/trans isomerase
OT  - photo-affinity labeling
EDAT- 2020/03/07 06:00
MHDA- 2021/06/03 06:00
CRDT- 2020/03/07 06:00
PHST- 2019/11/25 00:00 [received]
PHST- 2020/03/04 00:00 [accepted]
PHST- 2020/03/07 06:00 [pubmed]
PHST- 2021/06/03 06:00 [medline]
PHST- 2020/03/07 06:00 [entrez]
AID - /j/bchm.just-accepted/hsz-2019-0423/hsz-2019-0423.xml [pii]
AID - 10.1515/hsz-2019-0423 [doi]
PST - ppublish
SO  - Biol Chem. 2020 Jul 28;401(8):955-968. doi: 10.1515/hsz-2019-0423.