PMID- 32145086
OWN - NLM
STAT- MEDLINE
DCOM- 20200608
LR  - 20200608
IS  - 1365-3083 (Electronic)
IS  - 0300-9475 (Linking)
VI  - 91
IP  - 6
DP  - 2020 Jun
TI  - TRIM32 promotes inflammatory responses in rheumatoid arthritis fibroblast-like 
      synoviocytes.
PG  - e12876
LID - 10.1111/sji.12876 [doi]
AB  - Rheumatoid arthritis (RA) is a worldwide autoimmune disease. The study of its 
      aetiology and mechanism has always been a focus topic in medicine. This research 
      was designed to investigate the effect of E3 ubiquitin ligase tripartite motif 
      protein 32 (TRIM32) in rheumatoid arthritis (RA). We found in fibroblast-like 
      synoviocytes (FLS) of RA patients, the expression of TRIM32 was significantly 
      increased compared with its expression in osteoarthritis (OA) patients FLS. A 
      widely used pro-inflammatory stimuli tumour necrosis factor-alpha (TNF-alpha) was 
      found to promote TRIM32 expression in a time-dependent manner. Furthermore, we 
      observed that overexpression of TRIM32 aggravated the production of 
      pro-inflammatory cytokines in FLS, silencing of TRIM32 showed the consistent 
      results. In addition, TRIM32 was found to activate nuclear factor kappaB (NF-kappaB) 
      signalling pathway, and TRIM32 could interact with TNF receptor-associated factor 
      2 (TRAF2) to promote the K63-linked polyubiquitination of TRAF2 in RA-FLS. In 
      conclusion, we suggested that TRIM32 as a positive regulator of inflammatory 
      responses in RA-FLS.
CI  - (c) 2020 The Scandinavian Foundation for Immunology.
FAU - Liang, Tian
AU  - Liang T
AD  - Department of Orthopedics, Gansu Traditional Chinese Medicine University, 
      Lanzhou, China.
FAU - Song, Min
AU  - Song M
AD  - Department of Orthopedics, Gansu Traditional Chinese Medicine University, 
      Lanzhou, China.
FAU - Xu, Kewu
AU  - Xu K
AD  - Department of Orthopedics, Affiliated Hospital of Gansu Traditional Chinese 
      Medicine University, Lanzhou, China.
FAU - Guo, Chenglong
AU  - Guo C
AD  - Department of Orthopedics, Affiliated Hospital of Gansu Traditional Chinese 
      Medicine University, Lanzhou, China.
FAU - Xu, Hongbin
AU  - Xu H
AD  - Department of Orthopedics, Affiliated Hospital of Gansu Traditional Chinese 
      Medicine University, Lanzhou, China.
FAU - Zhang, Hongwei
AU  - Zhang H
AD  - Department of Orthopedics, Affiliated Hospital of Gansu Traditional Chinese 
      Medicine University, Lanzhou, China.
FAU - Xu, Lanping
AU  - Xu L
AUID- ORCID: 0000-0002-0272-0293
AD  - Department of Dermatology, Affiliated Hospital of Gansu Traditional Chinese 
      Medicine University, Lanzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20200320
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (NF-kappa B)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (Transcription Factors)
RN  - 0 (Tripartite Motif Proteins)
RN  - EC 2.3.2.27 (TRIM32 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Arthritis, Rheumatoid/immunology/*metabolism
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Fibroblasts/*immunology
MH  - Humans
MH  - Inflammation/immunology/*metabolism
MH  - Inflammation Mediators/metabolism
MH  - NF-kappa B/metabolism
MH  - Signal Transduction
MH  - Synoviocytes/*immunology
MH  - TNF Receptor-Associated Factor 2/metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - Tripartite Motif Proteins/genetics/*metabolism
MH  - Ubiquitin-Protein Ligases/genetics/*metabolism
MH  - Ubiquitination
MH  - Up-Regulation
OTO - NOTNLM
OT  - NF-kappaB
OT  - TRAF2
OT  - TRIM32
OT  - rheumatoid arthritis
EDAT- 2020/03/08 06:00
MHDA- 2020/06/09 06:00
CRDT- 2020/03/08 06:00
PHST- 2019/12/26 00:00 [received]
PHST- 2020/02/11 00:00 [revised]
PHST- 2020/03/02 00:00 [accepted]
PHST- 2020/03/08 06:00 [pubmed]
PHST- 2020/06/09 06:00 [medline]
PHST- 2020/03/08 06:00 [entrez]
AID - 10.1111/sji.12876 [doi]
PST - ppublish
SO  - Scand J Immunol. 2020 Jun;91(6):e12876. doi: 10.1111/sji.12876. Epub 2020 Mar 20.