PMID- 32146475
OWN - NLM
STAT- MEDLINE
DCOM- 20210917
LR  - 20210917
IS  - 1423-0267 (Electronic)
IS  - 0030-3755 (Linking)
VI  - 244
IP  - 1
DP  - 2021
TI  - RNA Sequencing and Bioinformatic Analysis on Retinoblastoma Revealing that Cell 
      Cycle Deregulation Is a Key Process in Retinoblastoma Tumorigenesis.
PG  - 51-59
LID - 10.1159/000506993 [doi]
AB  - PURPOSE: Retinoblastoma (RB) is a primary pediatric ocular malignancy that can be 
      fatal with inadequate treatment. While multimodal treatments are applied for eye 
      salvage, vision loss and metastasis can occur in some patients. The present study 
      aimed to explore key pathways and factors in RB pathogenesis, which could be 
      potential targets for novel RB treatments. METHODS: RNA sequencing was performed 
      on three RB tissues and referenced with three normal retinas. Differentially 
      expressed genes (DEGs) were identified from sequencing data and further analyzed 
      with clustering analysis, function and pathway enrichment, protein-protein 
      interaction (PPI), and data-mining analysis in order to screen for tumorigenic 
      relevancy. RESULTS: A total of 331 DEGs were identified by clustering analysis of 
      RB tissues, and the expression patterns were significantly distinguishable from 
      normal retinas. Function and pathway enrichment and PPI analysis together showed 
      that cell cycle was the most prominently upregulated pathway found in RB tissues. 
      Following comprehensive bioinformatic analyses, six key genes relevant to cell 
      cycle regulation were identified, namely BUB1, RRM2, TPX2, UBE2C, NUSAP1, and 
      DTL. CONCLUSIONS: Cell cycle pathway and six relevant genes may be potential key 
      factors in RB tumorigenesis and laying the foundation for prospective 
      investigation on development of novel targeted therapies.
CI  - (c) 2020 S. Karger AG, Basel.
FAU - Nie, Cong
AU  - Nie C
AD  - Department of Orbital Diseases and Ocular Oncology, State Key Laboratory of 
      Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, 
      China.
FAU - Ma, Huan
AU  - Ma H
AD  - Department of Orbital Diseases and Ocular Oncology, State Key Laboratory of 
      Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, 
      China.
FAU - Gao, Yang
AU  - Gao Y
AD  - Department of Orbital Diseases and Ocular Oncology, State Key Laboratory of 
      Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, 
      China.
FAU - Li, Jinmiao
AU  - Li J
AD  - Department of Orbital Diseases and Ocular Oncology, State Key Laboratory of 
      Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, 
      China.
FAU - Tang, Zhixin
AU  - Tang Z
AD  - Department of Orbital Diseases and Ocular Oncology, State Key Laboratory of 
      Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, 
      China.
FAU - Chen, Ying
AU  - Chen Y
AD  - Department of Orbital Diseases and Ocular Oncology, State Key Laboratory of 
      Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, 
      China.
FAU - Lu, Rong
AU  - Lu R
AD  - Department of Orbital Diseases and Ocular Oncology, State Key Laboratory of 
      Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, 
      China, lurong@gzzoc.com.
LA  - eng
PT  - Journal Article
DEP - 20200306
PL  - Switzerland
TA  - Ophthalmologica
JT  - Ophthalmologica. Journal international d'ophtalmologie. International journal of 
      ophthalmology. Zeitschrift fur Augenheilkunde
JID - 0054655
SB  - IM
MH  - Carcinogenesis/genetics
MH  - Cell Cycle/genetics
MH  - Child
MH  - Computational Biology
MH  - Humans
MH  - Prospective Studies
MH  - *Retinal Neoplasms/diagnosis/genetics
MH  - *Retinoblastoma/diagnosis/genetics
MH  - Sequence Analysis, RNA
OTO - NOTNLM
OT  - Bioinformatic analysis
OT  - Cell cycle
OT  - RNA sequencing
OT  - Retinoblastoma
EDAT- 2020/03/09 06:00
MHDA- 2021/09/18 06:00
CRDT- 2020/03/09 06:00
PHST- 2019/09/27 00:00 [received]
PHST- 2020/02/28 00:00 [accepted]
PHST- 2020/03/09 06:00 [pubmed]
PHST- 2021/09/18 06:00 [medline]
PHST- 2020/03/09 06:00 [entrez]
AID - 000506993 [pii]
AID - 10.1159/000506993 [doi]
PST - ppublish
SO  - Ophthalmologica. 2021;244(1):51-59. doi: 10.1159/000506993. Epub 2020 Mar 6.