PMID- 32146672
OWN - NLM
STAT- MEDLINE
DCOM- 20200710
LR  - 20200710
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 27
IP  - 14
DP  - 2020 May
TI  - Biomarker and hematological fieldwork with amphibians: is it necessary to sample 
      all night?
PG  - 17152-17161
LID - 10.1007/s11356-020-08313-2 [doi]
AB  - In the context of the global amphibian crisis, biomonitoring constitutes a 
      valuable assessment tool to provide critical up to date information on the status 
      and health of amphibians worldwide. The aim of the current study was to evaluate 
      the possible confounding effects of sex, size, and time since capture on 
      enzymatic biomarkers and hematologic parameters of the South American frog 
      Leptodactylus latrans. Frogs were collected by hand between 9 pm and 12 am on two 
      consecutive nights. On the first night, captured frogs were transported for 2 h 
      by car to laboratory installations, maintained overnight in plastic containers, 
      and blood and tissue sampled on the next morning. In contrast, frogs collected on 
      the second night were blood and tissue sampled in the field, immediately after 
      the capture period. Hematological parameters were analyzed, and enzymatic 
      activities of catalase, cholinesterase (ChE), and glutathione S-transferase (GST) 
      were determined in the plasma, liver, kidney, and muscle. A sex difference was 
      observed only for total white blood cell counts (WBC), females exhibiting 
      significantly greater values than males. The packed cell volume (PCV), mean 
      corpuscular hemoglobin concentration (MCHC), WBC, and muscle ChE activity were 
      significantly correlated with snout-vent length (SVL). The correlation was 
      inversed in the case of MCHC, WBC, and muscle ChE, while the correlation was 
      positive between PCV and SVL. Most examined parameters presented similar values 
      when frogs were sampled at night following capture or the next morning. Total red 
      blood cells (RBCs) count, and plasma enzymatic activities of ChE and GST were the 
      only parameters that presented significantly increased values in morning 
      samplings compared with night samplings. Overall, the current study indicates 
      that it is best to sample the frogs as soon as possible after capture if 
      hematologic or plasmatic biomarkers are examined. Nevertheless, it is possible to 
      sample on the next morning if tissular biomarkers are employed.
FAU - Brodeur, Julie Celine
AU  - Brodeur JC
AD  - Instituto de Recursos Biologicos, Centro de Investigaciones de Recursos Naturales 
      (CIRN), Centro Nacional de Investigaciones Agropecuarias (CNIA), Instituto 
      Nacional de Tecnologia Agropecuaria (INTA), 1686, Hurlingham, Buenos Aires, 
      Argentina. julbrodeur@hotmail.com.
AD  - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina. julbrodeur@hotmail.com.
FAU - Bahl, Maria Florencia
AU  - Bahl MF
AD  - Centro Investigaciones del Medio Ambiente, Departamento de Quimica, Facultad de 
      Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, 
      Argentina.
AD  - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina.
FAU - Natale, Guillermo Sebastian
AU  - Natale GS
AD  - Centro Investigaciones del Medio Ambiente, Departamento de Quimica, Facultad de 
      Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, 
      Argentina.
AD  - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina.
FAU - Poliserpi, Maria Belen
AU  - Poliserpi MB
AD  - Instituto de Recursos Biologicos, Centro de Investigaciones de Recursos Naturales 
      (CIRN), Centro Nacional de Investigaciones Agropecuarias (CNIA), Instituto 
      Nacional de Tecnologia Agropecuaria (INTA), 1686, Hurlingham, Buenos Aires, 
      Argentina.
LA  - eng
GR  - PNNAT-1128043/instituto nacional de tecnologia agropecuaria/
PT  - Journal Article
DEP - 20200307
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - *Anura
MH  - Biomarkers
MH  - Erythrocyte Count
MH  - *Erythrocyte Indices
MH  - Female
MH  - Hematocrit
MH  - Male
OTO - NOTNLM
OT  - Amphibians
OT  - Biomarkers
OT  - Contamination
OT  - Stress
EDAT- 2020/03/09 06:00
MHDA- 2020/07/11 06:00
CRDT- 2020/03/09 06:00
PHST- 2019/11/12 00:00 [received]
PHST- 2020/03/02 00:00 [accepted]
PHST- 2020/03/09 06:00 [pubmed]
PHST- 2020/07/11 06:00 [medline]
PHST- 2020/03/09 06:00 [entrez]
AID - 10.1007/s11356-020-08313-2 [pii]
AID - 10.1007/s11356-020-08313-2 [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2020 May;27(14):17152-17161. doi: 
      10.1007/s11356-020-08313-2. Epub 2020 Mar 7.