PMID- 32146812
OWN - NLM
STAT- MEDLINE
DCOM- 20210104
LR  - 20211204
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 68
IP  - 13
DP  - 2020 Apr 1
TI  - Differential Regulatory Roles of MicroRNAs in Porcine Intramuscular and 
      Subcutaneous Adipocytes.
PG  - 3954-3962
LID - 10.1021/acs.jafc.9b08191 [doi]
AB  - The deposition of intramuscular (IM) and subcutaneous (SC) fat is an important 
      trait influencing pork quality. Understanding the genetic differences between 
      these two types of adipose tissues is consequently of great importance for pig 
      breeding. Here, we established primary cultures of IM and SC adipocytes from 
      Jiaxing black pigs. The microRNA (miRNA) expression profiles of the two types of 
      adipocytes were obtained by RNA-seq. A total of 741 miRNAs were identified in IM 
      and SC adipocytes, including 155 significant differentially expressed (SDE) 
      miRNAs. According to gene ontology and Kyoto Encyclopedia of Genes analysis, the 
      target genes of the SDE miRNAs were enriched in categories and pathways related 
      to transcriptional regulation, fatty acid biosynthesis, as well as the MAPK and 
      PI3K/Akt pathways. Notably, miR-206 expression was 36-fold higher in IM 
      adipocytes than in SC adipocytes. The overexpression of miR-206 in IM and SC 
      adipocytes decreased cell proliferation and triglyceride accumulation. Luciferase 
      activity assays and quantitative polymerase chain reaction confirmed that miR-206 
      regulates adipocyte proliferation by targeting STARD7 and inhibits adipogenesis 
      by repressing Kruppel-like factor 4 (KLF4) expression. Accordingly, the effect of 
      miR-206 mimics was attenuated by the overexpression of KLF4 in adipocytes. Taken 
      together, we identified the expression profiles of miRNAs in adipocytes, which 
      revealed that miR-206 acts as a suppressor of adipogenesis.
FAU - Xu, Ke
AU  - Xu K
AD  - College of Biological, Chemical Sciences and Engineering, Jiaxing University, 
      Jiaxing 314001, China.
AD  - College of Agronomy and Biotechnology, Hebei Normal University of Science and 
      Technology, Qinhuangdao 066000, China.
FAU - Ji, Miao
AU  - Ji M
AD  - College of Biological, Chemical Sciences and Engineering, Jiaxing University, 
      Jiaxing 314001, China.
AD  - College of Agronomy and Biotechnology, Hebei Normal University of Science and 
      Technology, Qinhuangdao 066000, China.
FAU - Huang, Xin
AU  - Huang X
AD  - College of Biological, Chemical Sciences and Engineering, Jiaxing University, 
      Jiaxing 314001, China.
AD  - College of Agronomy and Biotechnology, Hebei Normal University of Science and 
      Technology, Qinhuangdao 066000, China.
FAU - Peng, Yongjia
AU  - Peng Y
AD  - College of Biological, Chemical Sciences and Engineering, Jiaxing University, 
      Jiaxing 314001, China.
FAU - Wu, Wenjing
AU  - Wu W
AUID- ORCID: 0000-0001-8479-0895
AD  - College of Biological, Chemical Sciences and Engineering, Jiaxing University, 
      Jiaxing 314001, China.
FAU - Zhang, Jin
AU  - Zhang J
AD  - College of Biological, Chemical Sciences and Engineering, Jiaxing University, 
      Jiaxing 314001, China.
LA  - eng
PT  - Journal Article
DEP - 20200318
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adipocytes/cytology/*metabolism
MH  - Animals
MH  - Cell Proliferation
MH  - Gene Expression Regulation
MH  - Kruppel-Like Factor 4
MH  - Kruppel-Like Transcription Factors/genetics/metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - Subcutaneous Fat/*cytology/metabolism
MH  - Swine
OTO - NOTNLM
OT  - RNA-seq
OT  - intramuscular adipocytes
OT  - miRNA
OT  - pork quality
OT  - subcutaneous adipocytes
EDAT- 2020/03/10 06:00
MHDA- 2021/01/05 06:00
CRDT- 2020/03/10 06:00
PHST- 2020/03/10 06:00 [pubmed]
PHST- 2021/01/05 06:00 [medline]
PHST- 2020/03/10 06:00 [entrez]
AID - 10.1021/acs.jafc.9b08191 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2020 Apr 1;68(13):3954-3962. doi: 10.1021/acs.jafc.9b08191. 
      Epub 2020 Mar 18.