PMID- 32147838
OWN - NLM
STAT- MEDLINE
DCOM- 20200831
LR  - 20200831
IS  - 1750-3841 (Electronic)
IS  - 0022-1147 (Linking)
VI  - 85
IP  - 4
DP  - 2020 Apr
TI  - Antioxidant, alpha-amylase and alpha-glucosidase activity of various solvent fractions of 
      I. obliquus and the preventive role of active fraction against H(2) O(2) induced 
      damage in hepatic L02 cells as fungisome.
PG  - 1060-1069
LID - 10.1111/1750-3841.15084 [doi]
AB  - Inonotus obliquus is a traditional mushroom well known for its therapeutic value. 
      In this study, various solvent fractions of I. obliquus were preliminarily 
      screened for their antioxidant, alpha-amylase and alpha-glucosidase inhibition 
      properties. To improve the drug delivery, the active fraction (ethyl acetate 
      fraction) of I. obliquus was synthesized into fungisome (ethyl acetate 
      phophotidyl choline complex, EAPC) and its physical parameters were assessed 
      using Fourier transform infrared spectroscopy (FTIR), High performance liquid 
      chromatography (HPLC), Scanning electron microscope (SEM), and varsigma potential 
      analysis. Then normal human hepatic L02 cells was used to evaluate the 
      cytotoxicity of EAPC. The results showed that EA fraction possesses significant 
      free radical scavenging, alpha-amylase and alpha-glucosidase inhibition properties. FTIR, 
      SEM, and HPLC analysis confirmed the fungisome formation. The particle size of 
      EAPC was 102.80 +/- 0.42 nm and the varsigma potential was -54.30 +/- 0.61 mV. The 
      percentage of drug entrapment efficiency was 97.13% and the drug release rates of 
      EAPC in simulated gastric fluid and simulated intestinal fluid were 75.04 +/- 0.29% 
      and 93.03 +/- 0.36%, respectively. EAPC was nontoxic to L02 cells, however it could 
      selectively fight against the H(2) O(2) induced oxidative damage in L02 cells. 
      This is the first study to provide scientific information to utilize the active 
      fraction of I. obliquus as fungisome. PRACTICAL APPLICATIONS: Inonotus obliquus 
      (IO) is a traditional medicinal fungus. The extracts of IO have obvious 
      antioxidant and hypoglycemic activities. Ethyl acetate (EA) fraction of IO was 
      encapsulated in liposomes to form EAPC. EAPC has a sustained-release effect. It 
      has nontoxic to L02 cells and could protect L02 cells from oxidative damage 
      caused by hydrogen peroxide. This study could provide new ideas for the treatment 
      of diabetes.
CI  - (c) 2020 Institute of Food Technologists(R).
FAU - Gao, Xudong
AU  - Gao X
AD  - Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of 
      Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, P.R. 
      China.
FAU - Santhanam, Ramesh Kumar
AU  - Santhanam RK
AD  - Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of 
      Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, P.R. 
      China.
AD  - School of Fundamental Science, University Malaysia Terengganu, 21030, Kuala 
      Nerus, Terengganu, Malaysia.
FAU - Xue, Zihan
AU  - Xue Z
AD  - Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of 
      Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, P.R. 
      China.
FAU - Jia, Yanan
AU  - Jia Y
AD  - Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of 
      Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, P.R. 
      China.
FAU - Wang, Yajie
AU  - Wang Y
AD  - Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of 
      Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, P.R. 
      China.
FAU - Lu, Yangpeng
AU  - Lu Y
AD  - Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of 
      Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, P.R. 
      China.
FAU - Phisalaphong, Muenduen
AU  - Phisalaphong M
AD  - Department of Chemical Engineering, Chulalongkorn University, Phayathai Road, 
      Bangkok, 10330, Pathum Wan, Thailand.
FAU - Chen, Haixia
AU  - Chen H
AUID- ORCID: 0000-0002-2596-9622
AD  - Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of 
      Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, P.R. 
      China.
LA  - eng
GR  - 31371879/National Natural Science Foundation of China/
GR  - 18PTZWHZ00190/Tianjin Municipal Science and Technology Foundation/
PT  - Journal Article
DEP - 20200309
PL  - United States
TA  - J Food Sci
JT  - Journal of food science
JID - 0014052
RN  - 0 (Antioxidants)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Plant Extracts)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 3.2.1.1 (alpha-Amylases)
RN  - EC 3.2.1.20 (alpha-Glucosidases)
SB  - IM
MH  - Agaricales/*chemistry
MH  - Antioxidants/chemistry/isolation & purification/*pharmacology
MH  - Basidiomycota/*chemistry
MH  - Cell Line
MH  - Enzyme Inhibitors/chemistry/isolation & purification/*pharmacology
MH  - Hepatocytes/drug effects/metabolism
MH  - Humans
MH  - Hydrogen Peroxide/*toxicity
MH  - Hypoglycemic Agents/chemistry/isolation & purification/pharmacology
MH  - Oxidative Stress/drug effects
MH  - Plant Extracts/chemistry/isolation & purification/*pharmacology
MH  - alpha-Amylases/*antagonists & inhibitors/chemistry
MH  - alpha-Glucosidases/chemistry
OTO - NOTNLM
OT  - Inonotus obliquus
OT  - L02 cells
OT  - fungisome
OT  - oxidative damage
EDAT- 2020/03/10 06:00
MHDA- 2020/09/01 06:00
CRDT- 2020/03/10 06:00
PHST- 2019/08/20 00:00 [received]
PHST- 2019/12/23 00:00 [revised]
PHST- 2020/01/25 00:00 [accepted]
PHST- 2020/03/10 06:00 [pubmed]
PHST- 2020/09/01 06:00 [medline]
PHST- 2020/03/10 06:00 [entrez]
AID - 10.1111/1750-3841.15084 [doi]
PST - ppublish
SO  - J Food Sci. 2020 Apr;85(4):1060-1069. doi: 10.1111/1750-3841.15084. Epub 2020 Mar 
      9.