PMID- 32148653
OWN - NLM
STAT- MEDLINE
DCOM- 20201019
LR  - 20211204
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2020
DP  - 2020
TI  - Autophagy Triggered by Oxidative Stress Appears to Be Mediated by the AKT/mTOR 
      Signaling Pathway in the Liver of Sleep-Deprived Rats.
PG  - 6181630
LID - 10.1155/2020/6181630 [doi]
LID - 6181630
AB  - Sleep deprivation adversely affects the digestive system. Multiple studies have 
      suggested sleep deprivation and oxidative stress are closely related. Autophagy 
      can be triggered by oxidative stress as a self-defense strategy to promote 
      survival. In this study, we investigated the effects of sleep deprivation on 
      liver functions, oxidative stress, and concomitant hepatocyte autophagy, as well 
      as the associated pathways. Enzymatic and nonenzymatic biochemical markers in the 
      serum were used to assess hepatic function and damage. To evaluate the occurrence 
      of autophagy, expression of autophagy-related proteins was tested and 
      autophagosomes were labeled. Additionally, methane dicarboxylic aldehyde (MDA), 
      antioxidant enzymes, and the protein kinase B (AKT)/mammalian target of rapamycin 
      (mTOR) signaling pathway were analyzed using chemical methods and a Western blot. 
      Serum alanine transaminase, aspartate aminotransferase, and alkaline phosphatase 
      increased in sleep-deprived rats. Total protein and albumin abundance was also 
      abnormal. Sleep deprivation induced histopathological changes in the liver. The 
      superoxide dismutase level decreased significantly in the liver of sleep-deprived 
      rats. In contrast, the MDA content increased in the sleep deprivation group. 
      Moreover, the microtubule-associated protein 1 light chain 3 beta (LC3B) II/I 
      ratio and Beclin I content increased considerably in the sleep-deprived rats, 
      while p62 levels decreased. Sleep deprivation apparently inhibited the AKT/mTOR 
      signaling pathway. We conclude that sleep deprivation can induce oxidative stress 
      and ultimately cause liver injury. Autophagy triggered by oxidative stress 
      appears to be mediated by the AKT/mTOR pathway and plays a role in relieving 
      oxidative stress caused by sleep deprivation.
CI  - Copyright (c) 2020 Yongmei Li et al.
FAU - Li, Yongmei
AU  - Li Y
AUID- ORCID: 0000-0003-1808-6060
AD  - NHC Key Laboratory of Hormones and Development (Tianjin Medical University), 
      Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University Chu 
      Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin, China.
FAU - Zhang, Yuan
AU  - Zhang Y
AUID- ORCID: 0000-0002-5019-8911
AD  - Basic Medical College, Tianjin Medical University, Tianjin, China.
FAU - Ji, Guang
AU  - Ji G
AUID- ORCID: 0000-0002-6349-5114
AD  - NHC Key Laboratory of Hormones and Development (Tianjin Medical University), 
      Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University Chu 
      Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin, China.
FAU - Shen, Yiwei
AU  - Shen Y
AD  - Basic Medical College, Tianjin Medical University, Tianjin, China.
FAU - Zhao, Nan
AU  - Zhao N
AD  - NHC Key Laboratory of Hormones and Development (Tianjin Medical University), 
      Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University Chu 
      Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin, China.
FAU - Liang, Yuhan
AU  - Liang Y
AD  - Basic Medical College, Tianjin Medical University, Tianjin, China.
FAU - Wang, Zihan
AU  - Wang Z
AUID- ORCID: 0000-0001-8193-7054
AD  - Basic Medical College, Tianjin Medical University, Tianjin, China.
FAU - Liu, Mengqi
AU  - Liu M
AD  - Basic Medical College, Tianjin Medical University, Tianjin, China.
FAU - Lin, Laixiang
AU  - Lin L
AUID- ORCID: 0000-0002-0100-4084
AD  - NHC Key Laboratory of Hormones and Development (Tianjin Medical University), 
      Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University Chu 
      Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin, China.
AD  - Qinghai Institute for Endemic Disease Prevention and Control, Qinghai, China.
LA  - eng
PT  - Journal Article
DEP - 20200213
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Autophagy
MH  - Liver/*physiopathology
MH  - Male
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction
MH  - Sleep Deprivation/*complications
MH  - TOR Serine-Threonine Kinases/*metabolism
PMC - PMC7044486
COIS- The authors declare that there is no conflict of interests regarding the 
      publication of this article.
EDAT- 2020/03/10 06:00
MHDA- 2020/10/21 06:00
PMCR- 2020/02/13
CRDT- 2020/03/10 06:00
PHST- 2019/04/04 00:00 [received]
PHST- 2019/10/22 00:00 [revised]
PHST- 2019/12/11 00:00 [accepted]
PHST- 2020/03/10 06:00 [entrez]
PHST- 2020/03/10 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2020/02/13 00:00 [pmc-release]
AID - 10.1155/2020/6181630 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2020 Feb 13;2020:6181630. doi: 10.1155/2020/6181630. 
      eCollection 2020.