PMID- 32151032 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200928 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 9 IP - 3 DP - 2020 Mar 5 TI - Influence of Preformed Antibodies in Liver Transplantation. LID - 10.3390/jcm9030708 [doi] LID - 708 AB - The significance of human leukocyte antigen (HLA) matching and preformed donor-specific antibodies (DSAs) in liver transplantation remains unclear. The aim of this study was to analyze the presence of DSAs in a large cohort of 810 liver recipients undergoing liver transplant to determine the influence on acute (AR) or chronic liver rejection (CR), graft loss and allograft survival. DSAs were identified using complement dependent cytotoxicity crossmatch (CDC-CM) and multiplexed solid-phase-based flow cytometry assay (Luminex). CDC-CM showed that a 3.2% of liver transplants were positive (+CDC-CM) with an AR frequency of 19.2% which was not different from that observed in negative patients (-CDC-CM, 22.3%). Only two patients transplanted with +CDC-CM (7.6%) developed CR and suffered re-transplant. +CDC-CM patients showed a significantly lower survival rate compared to -CDC-CM patients (23.1% vs. 59.1%, p = 0.0003), developing allograft failure within the first three months (p < 0.00001). In conclusion, we have demonstrated a relationship between the presence of preformed DSAs and the low graft liver survival, indicating the important role and the potential interest of performing this analysis before liver transplantation. Our results could help to detect patients with an increased risk of graft loss, a better choice of liver receptors as well as the establishment of individualized immunosuppressive regimens. FAU - Legaz, Isabel AU - Legaz I AUID- ORCID: 0000-0002-1140-4313 AD - Department of Legal and Forensic Medicine, Biomedical Research Institute (IMIB), Regional Campus of International Excellence "Campus Mare Nostrum", Faculty of Medicine, University of Murcia, 30100 Murcia, Spain. FAU - Boix, Francisco AU - Boix F AUID- ORCID: 0000-0002-3926-7920 AD - Immunology Service, Murcia Institute of Biosanitary Research (IMIB) and Biomedical Research Center in Liver and Digestive Diseases Network (CIBERehd), Virgen de la Arrixaca University Clinical Hospital (HCUVA), 30120 Murcia, Spain. FAU - Lopez, Manuela AU - Lopez M AD - Immunology Service, Murcia Institute of Biosanitary Research (IMIB) and Biomedical Research Center in Liver and Digestive Diseases Network (CIBERehd), Virgen de la Arrixaca University Clinical Hospital (HCUVA), 30120 Murcia, Spain. FAU - Alfaro, Rafael AU - Alfaro R AD - Immunology Service, Murcia Institute of Biosanitary Research (IMIB) and Biomedical Research Center in Liver and Digestive Diseases Network (CIBERehd), Virgen de la Arrixaca University Clinical Hospital (HCUVA), 30120 Murcia, Spain. FAU - Galian, Jose A AU - Galian JA AD - Immunology Service, Murcia Institute of Biosanitary Research (IMIB) and Biomedical Research Center in Liver and Digestive Diseases Network (CIBERehd), Virgen de la Arrixaca University Clinical Hospital (HCUVA), 30120 Murcia, Spain. FAU - Llorente, Santiago AU - Llorente S AD - Immunology Service, Murcia Institute of Biosanitary Research (IMIB) and Biomedical Research Center in Liver and Digestive Diseases Network (CIBERehd), Virgen de la Arrixaca University Clinical Hospital (HCUVA), 30120 Murcia, Spain. FAU - Campillo, Jose A AU - Campillo JA AD - Immunology Service, Murcia Institute of Biosanitary Research (IMIB) and Biomedical Research Center in Liver and Digestive Diseases Network (CIBERehd), Virgen de la Arrixaca University Clinical Hospital (HCUVA), 30120 Murcia, Spain. FAU - Botella, Carmen AU - Botella C AD - Immunology Service, Murcia Institute of Biosanitary Research (IMIB) and Biomedical Research Center in Liver and Digestive Diseases Network (CIBERehd), Virgen de la Arrixaca University Clinical Hospital (HCUVA), 30120 Murcia, Spain. FAU - Ramirez, Pablo AU - Ramirez P AD - Surgery Service, Murcia Institute of Biosanitary Research (IMIB) and Biomedical Research Center in Liver and Digestive Diseases Network (CIBERehd), Virgen de la Arrixaca University Clinical Hospital (HCUVA), 30120 Murcia, Spain. FAU - Sanchez-Bueno, Francisco AU - Sanchez-Bueno F AD - Surgery Service, Murcia Institute of Biosanitary Research (IMIB) and Biomedical Research Center in Liver and Digestive Diseases Network (CIBERehd), Virgen de la Arrixaca University Clinical Hospital (HCUVA), 30120 Murcia, Spain. FAU - Pons, Jose A AU - Pons JA AD - Digestive Medicine Service. Murcia Institute of Biosanitary Research (IMIB) and Biomedical Research Center in Liver and Digestive Diseases Network (CIBERehd), Virgen de la Arrixaca University Clinical Hospital (HCUVA), 30120 Murcia, Spain. FAU - Moya-Quiles, Maria R AU - Moya-Quiles MR AD - Immunology Service, Murcia Institute of Biosanitary Research (IMIB) and Biomedical Research Center in Liver and Digestive Diseases Network (CIBERehd), Virgen de la Arrixaca University Clinical Hospital (HCUVA), 30120 Murcia, Spain. FAU - Minguela, Alfredo AU - Minguela A AUID- ORCID: 0000-0003-2472-5893 AD - Immunology Service, Murcia Institute of Biosanitary Research (IMIB) and Biomedical Research Center in Liver and Digestive Diseases Network (CIBERehd), Virgen de la Arrixaca University Clinical Hospital (HCUVA), 30120 Murcia, Spain. FAU - Muro, Manuel AU - Muro M AD - Immunology Service, Murcia Institute of Biosanitary Research (IMIB) and Biomedical Research Center in Liver and Digestive Diseases Network (CIBERehd), Virgen de la Arrixaca University Clinical Hospital (HCUVA), 30120 Murcia, Spain. LA - eng GR - PI15/01370/Spanish Ministry of Economy and Competitiveness/ GR - PI11/02686/Instituto de Salud Carlos III (ISCIII)/ GR - PI19/01194/European Fund of Regional Development (FEDER) with the principle of "A manner to build Europe"./ PT - Journal Article DEP - 20200305 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC7141359 OTO - NOTNLM OT - alcoholic cirrhosis OT - causes of death OT - crossmatch OT - donor-specific antibodies OT - forensic medicine OT - liver rejection OT - medico-legal autopsy OT - survival COIS- The authors declare no conflict of interest. EDAT- 2020/03/11 06:00 MHDA- 2020/03/11 06:01 PMCR- 2020/03/05 CRDT- 2020/03/11 06:00 PHST- 2020/02/14 00:00 [received] PHST- 2020/03/02 00:00 [accepted] PHST- 2020/03/11 06:00 [entrez] PHST- 2020/03/11 06:00 [pubmed] PHST- 2020/03/11 06:01 [medline] PHST- 2020/03/05 00:00 [pmc-release] AID - jcm9030708 [pii] AID - jcm-09-00708 [pii] AID - 10.3390/jcm9030708 [doi] PST - epublish SO - J Clin Med. 2020 Mar 5;9(3):708. doi: 10.3390/jcm9030708.