PMID- 32153363
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220129
IS  - 1662-4548 (Print)
IS  - 1662-453X (Electronic)
IS  - 1662-453X (Linking)
VI  - 14
DP  - 2020
TI  - Simultaneous Electrophysiology and Fiber Photometry in Freely Behaving Mice.
PG  - 148
LID - 10.3389/fnins.2020.00148 [doi]
LID - 148
AB  - In vivo electrophysiology is the gold standard technique used to investigate 
      sub-second neural dynamics in freely behaving animals. However, monitoring 
      cell-type-specific population activity is not a trivial task. Over the last 
      decade, fiber photometry based on genetically encoded calcium indicators (GECIs) 
      has been widely adopted as a versatile tool to monitor cell-type-specific 
      population activity in vivo. However, this approach suffers from low temporal 
      resolution. Here, we combine these two approaches to monitor both sub-second 
      field potentials and cell-type-specific population activity in freely behaving 
      mice. By developing an economical custom-made system and constructing a hybrid 
      implant of an electrode and a fiber optic cannula, we simultaneously monitor 
      artifact-free mesopontine field potentials and calcium transients in cholinergic 
      neurons across the sleep-wake cycle. We find that mesopontine cholinergic 
      activity co-occurs with sub-second pontine waves, called P-waves, during rapid 
      eye movement sleep. Given the simplicity of our approach, simultaneous 
      electrophysiological recording and cell-type-specific imaging provides a novel 
      and valuable tool for interrogating state-dependent neural circuit dynamics in 
      vivo.
CI  - Copyright (c) 2020 Patel, McAlinden, Mathieson and Sakata.
FAU - Patel, Amisha A
AU  - Patel AA
AD  - Strathclyde Institute of Pharmacy & Biomedical Sciences, University of 
      Strathclyde, Glasgow, United Kingdom.
FAU - McAlinden, Niall
AU  - McAlinden N
AD  - Department of Physics, Institute of Photonics, SUPA, University of Strathclyde, 
      Glasgow, United Kingdom.
FAU - Mathieson, Keith
AU  - Mathieson K
AD  - Department of Physics, Institute of Photonics, SUPA, University of Strathclyde, 
      Glasgow, United Kingdom.
FAU - Sakata, Shuzo
AU  - Sakata S
AD  - Strathclyde Institute of Pharmacy & Biomedical Sciences, University of 
      Strathclyde, Glasgow, United Kingdom.
LA  - eng
GR  - S45/ACT_/RNID/United Kingdom
PT  - Journal Article
DEP - 20200221
PL  - Switzerland
TA  - Front Neurosci
JT  - Frontiers in neuroscience
JID - 101478481
PMC - PMC7047771
OTO - NOTNLM
OT  - GCaMP
OT  - REM sleep
OT  - acetylcholine
OT  - brain state
OT  - brainstem
OT  - pontine waves
EDAT- 2020/03/11 06:00
MHDA- 2020/03/11 06:01
PMCR- 2020/01/01
CRDT- 2020/03/11 06:00
PHST- 2019/10/16 00:00 [received]
PHST- 2020/02/07 00:00 [accepted]
PHST- 2020/03/11 06:00 [entrez]
PHST- 2020/03/11 06:00 [pubmed]
PHST- 2020/03/11 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fnins.2020.00148 [doi]
PST - epublish
SO  - Front Neurosci. 2020 Feb 21;14:148. doi: 10.3389/fnins.2020.00148. eCollection 
      2020.