PMID- 32153783
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220413
IS  - 2052-6113 (Print)
IS  - 2052-6113 (Electronic)
IS  - 2052-6113 (Linking)
VI  - 7
IP  - 1
DP  - 2020
TI  - Relationship between expression pattern of vitamin D receptor, 1 
      alpha-hydroxylase enzyme, and chemokine RANTES genes and selected serum 
      parameters during transition period in Holstein dairy cows.
PG  - e000339
LID - 10.1136/vetreco-2019-000339 [doi]
LID - e000339
AB  - OBJECTIVES: The objective of the present study was to evaluate the pattern of 
      genetic expression of vitamin D receptor (VDR), 1 alpha-hydroxylase (1alpha-OHase) 
      enzyme and chemokine regulated on activation normal T-cell expressed and secreted 
      (RANTES) in peripheral blood of Holstein dairy cows during transition period. 
      METHODS: Blood samples were collected from 16 Holstein dairy cows at 3 weeks 
      prior expected date of delivery (EDD), at the day of parturition, and 3 weeks 
      post-partum for assessment of expression profile of studied genes using real-time 
      PCR and measurement of glucose, ionized calcium (Ca), parathyroid hormone (PTH), 
      inorganic phosphorous (P), sodium (Na), potassium (K), chloride (Cl), and 
      magnesium (Mg) levels. RESULTS: Compared with 3 weeks prior EDD, VDR gene 
      expression decreased significantly at the day of parturition then increased 
      significantly at 3 weeks post-partum. The genetic expression of 1alpha-OHase enzyme 
      as well as PTH, K, Na and Cl levels increased significantly at the day of 
      parturition. The Ca level decreased significantly at the day of parturition then 
      increased significantly at 3 weeks post-partum. The P level increased 
      significantly at the day of parturition then decreased significantly at 3 weeks 
      post-partum. Glucose level decreased significantly at the day of parturition and 
      at 3 weeks post-partum. RANTES gene expression showed non-significant changes 
      among the three different time points. The expression of VDR gene had a negative 
      correlation with the expression of 1alpha-OHase enzyme gene, and serum levels of 
      glucose, PTH, P and K, but had a positive correlation with the serum Ca level. 
      The expression of 1alpha-OHase enzyme gene had a positive correlation with serum 
      levels of PTH, P and K, but had a negative correlation with the serum Ca level. 
      CONCLUSIONS: Results of the current study indicate the importance of monitoring 
      the genetic expression of VDR and 1alpha-OHase enzyme as indicators of metabolic 
      changes during transition period, suggesting that they are candidate genes to 
      judge the health status of dairy cows during such period.
CI  - (c) British Veterinary Association 2020. Re-use permitted under CC BY-NC. No 
      commercial re-use. Published by BMJ.
FAU - Saed, Hala A R
AU  - Saed HAR
AD  - Department of Internal medicine, Veterinary Teaching Hospital, Faculty of 
      Veterinary Medicine, Mansoura University, Mansoura, Egypt.
FAU - Ibrahim, Hussam M M
AU  - Ibrahim HMM
AUID- ORCID: 0000-0002-3731-4586
AD  - Department of Internal Medicine, Infectious and Fish Diseases, Faculty of 
      Veterinary Medicine, Mansoura University, Mansoura, Egypt.
FAU - El-Khodery, Sabry A
AU  - El-Khodery SA
AUID- ORCID: 0000-0002-0545-5827
AD  - Department of Internal Medicine, Infectious and Fish Diseases, Faculty of 
      Veterinary Medicine, Mansoura University, Mansoura, Egypt.
FAU - Youssef, Mohamed A
AU  - Youssef MA
AD  - Department of Internal Medicine, Infectious and Fish Diseases, Faculty of 
      Veterinary Medicine, Mansoura University, Mansoura, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20200109
PL  - United States
TA  - Vet Rec Open
JT  - Veterinary record open
JID - 101653671
PMC - PMC7045247
OTO - NOTNLM
OT  - clinical practice
OT  - dairy cattle
OT  - internal medicine
COIS- Competing interests: The authors have declared that they have no conflict of 
      interest.
EDAT- 2020/03/11 06:00
MHDA- 2020/03/11 06:01
PMCR- 2020/01/09
CRDT- 2020/03/11 06:00
PHST- 2019/01/25 00:00 [received]
PHST- 2019/11/24 00:00 [revised]
PHST- 2019/11/27 00:00 [accepted]
PHST- 2020/03/11 06:00 [entrez]
PHST- 2020/03/11 06:00 [pubmed]
PHST- 2020/03/11 06:01 [medline]
PHST- 2020/01/09 00:00 [pmc-release]
AID - vetreco-2019-000339 [pii]
AID - 10.1136/vetreco-2019-000339 [doi]
PST - epublish
SO  - Vet Rec Open. 2020 Jan 9;7(1):e000339. doi: 10.1136/vetreco-2019-000339. 
      eCollection 2020.