PMID- 32156075 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20231103 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 8 IP - 3 DP - 2020 Mar 7 TI - Beneficial Propionibacteria within a Probiotic Emmental Cheese: Impact on Dextran Sodium Sulphate-Induced Colitis in Mice. LID - 10.3390/microorganisms8030380 [doi] LID - 380 AB - BACKGROUNDS AND AIMS: Inflammatory Bowel Diseases (IBD), including Ulcerative Colitis (UC), coincide with alterations in the gut microbiota. Consumption of immunomodulatory strains of probiotic bacteria may induce or prolong remission in UC patients. Fermented foods, including cheeses, constitute major vectors for bacteria consumption. New evidences revealed anti-inflammatory effects in selected strains of Propionibacterium freudenreichii. We thus hypothesized that consumption of a functional cheese, fermented by such a strain, may exert a positive effect on IBD. METHODS: We investigated the impact of cheese fermented by P. freudenreichii on gut inflammation. We developed an experimental single-strain cheese solely fermented by a selected immunomodulatory strain of P. freudenreichii, CIRM-BIA 129. We moreover produced, in industrial conditions, an Emmental cheese using the same strain, in combination with Lactobacillus delbrueckii CNRZ327 and Streptococcus thermophilus LMD-9, as starters. Consumption of both cheeses was investigated with respect to prevention of Dextran Sodium Sulphate (DSS)-induced colitis in mice. RESULTS: Consumption of the single-strain experimental cheese, or of the industrial Emmental, both fermented by P. freudenreichii CIRM-BIA 129, reduced severity of subsequent DSS-induced colitis, weight loss, disease activity index and histological score. Both treatments, in a preventive way, reduced small bowel Immunoglobulin A (IgA) secretion, restored occludin gene expression and prevented induction of Tumor Necrosis Factor alpha (TNFalpha), Interferon gamma (IFNgamma) and Interleukin-17 (IL-17). CONCLUSIONS: A combination of immunomodulatory strains of starter bacteria can be used to manufacture an anti-inflammatory cheese, as revealed in an animal model of colitis. This opens new perspectives for personalised nutrition in the context of IBD. FAU - Rabah, Houem AU - Rabah H AD - STLO, INRA, Agrocampus Ouest, 35 000 Rennes, France. AD - Pole Agronomique Ouest, Regions Bretagne et Pays de la Loire, F-35 042 Rennes, France. FAU - do Carmo, Fillipe Luiz Rosa AU - do Carmo FLR AUID- ORCID: 0000-0003-4405-1013 AD - Departamento de Genetica, Ecologia e evolucao, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais CP 486 CEP 31270-901, Brazil. FAU - Carvalho, Rodrigo Dias de Oliveira AU - Carvalho RDO AD - I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow 119146, Russia. FAU - Cordeiro, Barbara Fernandes AU - Cordeiro BF AD - Departamento de Genetica, Ecologia e evolucao, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais CP 486 CEP 31270-901, Brazil. FAU - da Silva, Sara Heloisa AU - da Silva SH AD - Departamento de Genetica, Ecologia e evolucao, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais CP 486 CEP 31270-901, Brazil. FAU - Oliveira, Emiliano Rosa AU - Oliveira ER AD - Departamento de Genetica, Ecologia e evolucao, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais CP 486 CEP 31270-901, Brazil. FAU - Lemos, Luisa AU - Lemos L AD - Departamento de Genetica, Ecologia e evolucao, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais CP 486 CEP 31270-901, Brazil. FAU - Cara, Denise Carmona AU - Cara DC AD - Departamento de Genetica, Ecologia e evolucao, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais CP 486 CEP 31270-901, Brazil. FAU - Faria, Ana Maria Caetano AU - Faria AMC AD - Departamento de Genetica, Ecologia e evolucao, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais CP 486 CEP 31270-901, Brazil. FAU - Garric, Gilles AU - Garric G AD - STLO, INRA, Agrocampus Ouest, 35 000 Rennes, France. FAU - Harel-Oger, Marielle AU - Harel-Oger M AD - STLO, INRA, Agrocampus Ouest, 35 000 Rennes, France. FAU - Le Loir, Yves AU - Le Loir Y AD - STLO, INRA, Agrocampus Ouest, 35 000 Rennes, France. FAU - Azevedo, Vasco AU - Azevedo V AUID- ORCID: 0000-0002-4775-2280 AD - Departamento de Genetica, Ecologia e evolucao, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais CP 486 CEP 31270-901, Brazil. FAU - Bouguen, Guillaume AU - Bouguen G AD - CHU Rennes, Univ Rennes, INSERM, CIC1414, Institut NUMECAN (Nutrition Metabolism and Cancer), F-35000 Rennes, France. FAU - Jan, Gwenael AU - Jan G AD - STLO, INRA, Agrocampus Ouest, 35 000 Rennes, France. LA - eng PT - Journal Article DEP - 20200307 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC7142753 OTO - NOTNLM OT - Emmental OT - cheese OT - colitis OT - inflammation OT - inflammatory bowel disease OT - intestine OT - probiotic OT - propionibacteria COIS- The authors declare no conflict of interest. EDAT- 2020/03/12 06:00 MHDA- 2020/03/12 06:01 PMCR- 2020/03/07 CRDT- 2020/03/12 06:00 PHST- 2020/01/17 00:00 [received] PHST- 2020/03/03 00:00 [revised] PHST- 2020/03/05 00:00 [accepted] PHST- 2020/03/12 06:00 [entrez] PHST- 2020/03/12 06:00 [pubmed] PHST- 2020/03/12 06:01 [medline] PHST- 2020/03/07 00:00 [pmc-release] AID - microorganisms8030380 [pii] AID - microorganisms-08-00380 [pii] AID - 10.3390/microorganisms8030380 [doi] PST - epublish SO - Microorganisms. 2020 Mar 7;8(3):380. doi: 10.3390/microorganisms8030380.