PMID- 32160147
OWN - NLM
STAT- MEDLINE
DCOM- 20200508
LR  - 20200508
IS  - 1466-5034 (Electronic)
IS  - 1466-5026 (Linking)
VI  - 70
IP  - 3
DP  - 2020 Mar
TI  - Reclassification of Francisella noatunensis subsp. orientalis Ottem et al. 2009 
      as Francisella orientalis sp. nov., Francisella noatunensis subsp. chilensis 
      subsp. nov. and emended description of Francisella noatunensis.
PG  - 2034-2048
LID - 10.1099/ijsem.0.004009 [doi]
AB  - Francisella noatunensis is a fastidious facultative intracellular bacterial 
      pathogen that causes 'piscine francisellosis', a serious disease affecting both 
      marine and fresh water farmed and wild fish worldwide. Currently two F. 
      noatunensis subspecies are recognized, i.e. F. noatunensis subsp. noatunensis and 
      F. noatunensis subsp. orientalis. In the present study, the taxonomy of F. 
      noatunensis was revisited using a polyphasic approach, including whole genome 
      derived parameters such as digital DNA-DNA hybridization, whole genome average 
      nucleotide identity (wg-ANIm), whole genome phylogenetic analysis, whole genome 
      G+C content, metabolic fingerprinting and chemotaxonomic analyses. The results 
      indicated that isolates belonging to F. noatunensis subsp. orientalis represent a 
      phenotypically and genetically homogenous taxon, clearly distinguishable from F. 
      noatunensis subsp. noatunensis that fulfils requirements for separate species 
      status. We propose, therefore, elevation of F. noatunensis subsp. orientalis to 
      the species rank as Francisella orientalis sp. nov. with the type strain 
      remaining as Ehime-1(T) (DSM 21254(T)=LMG 24544(T)). Furthermore, we identified 
      sufficient phenotypic and genetic differences between F. noatunensis subsp. 
      noatunensis recovered from diseased farmed Atlantic salmon in Chile and those 
      isolated from wild and farmed Atlantic cod in Northern Europe to warrant proposal 
      of the Chilean as a novel F. noatunensis subspecies, i.e. Francisella noatunensis 
      subsp. chilensis subsp. nov. with strain PQ1106(T) (CECT 9798(T)=NCTC14375(T)) as 
      the type strain. Finally, we emend the description of F. noatunensis by including 
      further metabolic information and the description of atypical strains.
FAU - Ramirez-Paredes, Jose Gustavo
AU  - Ramirez-Paredes JG
AD  - Ridgeway Biologicals Ltd. a Ceva Sante Animale Company, Units 1-3 Old Station 
      Business Park, Compton, Berkshire, England, RG20 6NE, UK.
AD  - Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, 
      Stirling, Scotland, FK9 4LA, UK.
AD  - Present address: Ridgeway Biologicals Ltd. a Ceva Sante Animale Company, Units 
      1-3 Old Station Business Park, Compton, Berkshire, England, RG20 6NE, UK.
FAU - Larsson, Par
AU  - Larsson P
AD  - Swedish Defence Research Agency (FOI), CBRN Defence and Security, Umea, Sweden.
AD  - Department of Medical Biosciences, Umea University, Umea, Sweden.
FAU - Thompson, Kim D
AU  - Thompson KD
AD  - Aquaculture Research Group, Moredun Research Institute, Edinburgh, Scotland, EH26 
      0PZ, UK.
FAU - Penman, David J
AU  - Penman DJ
AD  - Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, 
      Stirling, Scotland, FK9 4LA, UK.
FAU - Busse, Hans-Jurgen
AU  - Busse HJ
AD  - Institute of Microbiology, Veterinary University, Vienna, A-1160 Wien, Austria.
FAU - Ohrman, Caroline
AU  - Ohrman C
AD  - Swedish Defence Research Agency (FOI), CBRN Defence and Security, Umea, Sweden.
FAU - Sjodin, Andreas
AU  - Sjodin A
AD  - Swedish Defence Research Agency (FOI), CBRN Defence and Security, Umea, Sweden.
FAU - Soto, Esteban
AU  - Soto E
AD  - Department of Medicine and Epidemiology, University of California Davis, School 
      of Veterinary Medicine, Davis, California, 95616, USA.
FAU - Richards, Randolph H
AU  - Richards RH
AD  - Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, 
      Stirling, Scotland, FK9 4LA, UK.
FAU - Adams, Alexandra
AU  - Adams A
AD  - Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, 
      Stirling, Scotland, FK9 4LA, UK.
FAU - Colquhoun, Duncan J
AU  - Colquhoun DJ
AD  - Fish Health Research Group, Norwegian Veterinary Institute, Oslo, Pb 750 Sentrum, 
      N-0106 Oslo, Norway.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Syst Evol Microbiol
JT  - International journal of systematic and evolutionary microbiology
JID - 100899600
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - Francisella noatunensis
SB  - IM
MH  - Animals
MH  - Bacterial Typing Techniques
MH  - Chile
MH  - DNA, Bacterial/genetics
MH  - Europe
MH  - Fish Diseases/microbiology
MH  - Fishes/microbiology
MH  - Francisella/*classification
MH  - Gram-Negative Bacterial Infections/veterinary
MH  - Nucleic Acid Hybridization
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Francisella new species
OT  - Francisella noatunensis subsp. chilensis
OT  - Francisella orientalis
OT  - Francisella salmon Chile
OT  - Francisella tilapia
OT  - tilapia diseases
EDAT- 2020/03/12 06:00
MHDA- 2020/05/10 06:00
CRDT- 2020/03/12 06:00
PHST- 2020/03/12 06:00 [pubmed]
PHST- 2020/05/10 06:00 [medline]
PHST- 2020/03/12 06:00 [entrez]
AID - 10.1099/ijsem.0.004009 [doi]
PST - ppublish
SO  - Int J Syst Evol Microbiol. 2020 Mar;70(3):2034-2048. doi: 10.1099/ijsem.0.004009.