PMID- 32162612
OWN - NLM
STAT- MEDLINE
DCOM- 20200424
LR  - 20200424
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 138
DP  - 2020 Mar 12
TI  - First comparison of French and Australian OsHV-1 microvars by bath exposure.
PG  - 137-144
LID - 10.3354/dao03452 [doi]
AB  - Economically devastating mortality events of farmed and wild shellfish due to 
      infectious disease have been reported globally. Currently, one of the most 
      significant disease threats to Pacific oyster Crassostrea gigas culture is the 
      ostreid herpesvirus 1 (OsHV-1), in particular the emerging OsHV-1 microvariant 
      genotypes. OsHV-1 microvariants (OsHV-1 microvars) are spreading globally, and 
      concern is high among growers in areas unaffected by OsHV-1. No study to date has 
      compared the relative virulence among variants. We provide the first challenge 
      study comparing survival of naive juvenile Pacific oysters exposed to OsHV-1 
      microvars from Australia (AUS microvar) and France (FRA microvar). Oysters challenged with 
      OsHV-1 microvars had low survival (2.5% exposed to AUS microvar and 10% to FRA microvar), and 
      high viral copy number as compared to control oysters (100% survival and no virus 
      detected). As our study was conducted in a quarantine facility located ~320 km 
      from the ocean, we also compared the virulence of OsHV-1 microvars using artificial 
      seawater made from either facility tap water (3782 micromol kg-1 seawater total 
      alkalinity) or purchased distilled water (2003 micromol kg-1). Although no 
      differences in survival or viral copy number were detected in oysters exposed to 
      seawater made using tap or distilled water, more OsHV-1 was detected in tanks 
      containing the lower-alkalinity seawater, indicating that water quality may be 
      important for virus transmission, as it may influence the duration of viral 
      viability outside of the host.
FAU - Burge, Colleen A
AU  - Burge CA
AD  - Institute of Marine and Environmental Technology, University of Maryland 
      Baltimore County, 701 E Pratt Street, Baltimore, Maryland 21202, USA.
FAU - Reece, Kimberly S
AU  - Reece KS
FAU - Dhar, Arun K
AU  - Dhar AK
FAU - Kirkland, Peter
AU  - Kirkland P
FAU - Morga, Benjamin
AU  - Morga B
FAU - Degremont, Lionel
AU  - Degremont L
FAU - Faury, Nicole
AU  - Faury N
FAU - Wippel, Bryanda J T
AU  - Wippel BJT
FAU - MacIntyre, Alanna
AU  - MacIntyre A
FAU - Friedman, Carolyn S
AU  - Friedman CS
LA  - eng
PT  - Journal Article
DEP - 20200312
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Australia
MH  - Crassostrea
MH  - DNA, Viral
MH  - France
MH  - *Herpesviridae
MH  - Seawater
OTO - NOTNLM
OT  - Alkalinity
OT  - Crassostrea gigas
OT  - Emerging infectious diseases
OT  - Microvariant
OT  - OsHV-1 microvars
OT  - Ostreid herpesvirus 1
OT  - POMS
OT  - Pacific oyster
OT  - Viral disease
OT  - qPCR
EDAT- 2020/03/13 06:00
MHDA- 2020/04/25 06:00
CRDT- 2020/03/13 06:00
PHST- 2020/03/13 06:00 [entrez]
PHST- 2020/03/13 06:00 [pubmed]
PHST- 2020/04/25 06:00 [medline]
AID - 10.3354/dao03452 [doi]
PST - epublish
SO  - Dis Aquat Organ. 2020 Mar 12;138:137-144. doi: 10.3354/dao03452.