PMID- 32162675
OWN - NLM
STAT- MEDLINE
DCOM- 20210827
LR  - 20210827
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 117
IP  - 6
DP  - 2020 Jun
TI  - High-throughput single-molecule imaging system using nanofabricated trenches and 
      fluorescent DNA-binding proteins.
PG  - 1640-1648
LID - 10.1002/bit.27331 [doi]
AB  - DNA curtain is a high-throughput system, integrating a lipid bilayer, 
      fluorescence imaging, and microfluidics to probe protein-DNA interactions in 
      real-time and has provided in-depth understanding of DNA metabolism. Especially, 
      the microfluidic platform of a DNA curtain is highly suitable for a biochip. In 
      the DNA curtain, DNA molecules are aligned along chromium nanobarriers, which are 
      fabricated on a slide surface, and visualized using an intercalating dye, YOYO-1. 
      Although the chromium barriers confer precise geometric alignment of DNA, reuse 
      of the slides is limited by wear of the barriers during cleaning. YOYO-1 is 
      rapidly photobleached and causes photocleavage of DNA under continuous laser 
      illumination, restricting DNA observation to a brief time window. To address 
      these challenges, we developed a new nanopatterned slide, upon which carved 
      nanotrenches serve as diffusion barriers. The nanotrenches were robust under 
      harsh cleaning conditions, facilitating the maintenance of surface cleanliness 
      that is essential to slide reuse. We also stained DNA with a fluorescent protein 
      with a DNA-binding motif, fluorescent protein-DNA binding peptide (FP-DBP). 
      FP-DBP was slowly photobleached and did not cause DNA photocleavage. This new DNA 
      curtain system enables a more stable and repeatable investigation of real-time 
      protein-DNA interactions and will serve as a good platform for lab-on-a-chip.
CI  - (c) 2020 Wiley Periodicals, Inc.
FAU - Kang, Yujin
AU  - Kang Y
AD  - School of Life Sciences, Ulsan National Institute of Science and Technology, 
      Ulsan, Republic of Korea.
FAU - Cheon, Na Young
AU  - Cheon NY
AD  - School of Life Sciences, Ulsan National Institute of Science and Technology, 
      Ulsan, Republic of Korea.
FAU - Cha, Jongjin
AU  - Cha J
AD  - Department of Physics and Astronomy, Seoul National University, Seoul, Republic 
      of Korea.
FAU - Kim, Ayoung
AU  - Kim A
AD  - School of Life Sciences, Ulsan National Institute of Science and Technology, 
      Ulsan, Republic of Korea.
FAU - Kim, Hyung-Il
AU  - Kim HI
AD  - UNIST Central Research Facilities (UCRF), Ulsan National Institute of Science and 
      Technology, Ulsan, Republic of Korea.
FAU - Lee, Luda
AU  - Lee L
AD  - UNIST Central Research Facilities (UCRF), Ulsan National Institute of Science and 
      Technology, Ulsan, Republic of Korea.
FAU - Kim, Kang O
AU  - Kim KO
AD  - UNIST Central Research Facilities (UCRF), Ulsan National Institute of Science and 
      Technology, Ulsan, Republic of Korea.
FAU - Jo, Kyubong
AU  - Jo K
AD  - Department of Chemistry and Integrated Biotechnology, Sogang University, Seoul, 
      Republic of Korea.
FAU - Lee, Ja Yil
AU  - Lee JY
AUID- ORCID: 0000-0002-5726-9022
AD  - School of Life Sciences, Ulsan National Institute of Science and Technology, 
      Ulsan, Republic of Korea.
AD  - Center for Genomic Integrity, Institute for Basic Science, Ulsan, Republic of 
      Korea.
LA  - eng
GR  - IBS-R022-D1/Institute for Basic Science/International
GR  - 1.160106.01/Ulsan National Institute of Science and Technology/International
GR  - POSCO Science Fellowship of POSCO TJ Park Foundation/International
GR  - NRF-2016R1A6A1A03012845/National Research Foundation of Korea/International
GR  - NRF-2017R1A2B4002213/National Research Foundation of Korea/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200406
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Benzoxazoles)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Lipid Bilayers)
RN  - 0 (Quinolinium Compounds)
RN  - 143413-85-8 
      (1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Benzoxazoles/*analysis
MH  - DNA/*analysis
MH  - DNA-Binding Proteins/*analysis
MH  - Fluorescent Dyes/*analysis
MH  - Lipid Bilayers/chemistry
MH  - Nanostructures/*chemistry
MH  - Optical Imaging/methods
MH  - Quinolinium Compounds/*analysis
MH  - Single Molecule Imaging/*methods
OTO - NOTNLM
OT  - DNA curtain
OT  - FP-DBP
OT  - high-throughput single-molecule imaging
OT  - nanotrench
EDAT- 2020/03/13 06:00
MHDA- 2021/08/28 06:00
CRDT- 2020/03/13 06:00
PHST- 2020/01/16 00:00 [received]
PHST- 2020/03/02 00:00 [revised]
PHST- 2020/03/11 00:00 [accepted]
PHST- 2020/03/13 06:00 [pubmed]
PHST- 2021/08/28 06:00 [medline]
PHST- 2020/03/13 06:00 [entrez]
AID - 10.1002/bit.27331 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2020 Jun;117(6):1640-1648. doi: 10.1002/bit.27331. Epub 2020 
      Apr 6.