PMID- 32163895 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20210110 IS - 2162-2531 (Print) IS - 2162-2531 (Electronic) IS - 2162-2531 (Linking) VI - 20 DP - 2020 Jun 5 TI - Long Noncoding RNA RC3H2 Facilitates Cell Proliferation and Invasion by Targeting MicroRNA-101-3p/EZH2 Axis in OSCC. PG - 97-110 LID - S2162-2531(20)30078-0 [pii] LID - 10.1016/j.omtn.2020.02.006 [doi] AB - In our previous studies, enhancer of zeste homolog 2 (EZH2) has been proven to be a key oncogenic driver in oral squamous cell carcinoma (OSCC). However, the regulatory mechanisms on EZH2 remain poorly understood in OSCC. Here, through multi-transcriptomics, bioinformatics analysis, and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), the co-expression network of long noncoding RNA RC3H2 (RC3H2), microRNA-101-3p (miR-101-3p), and EZH2 were screened and validated as a competing endogenous RNA (ceRNA) mechanism in OSCC. Silencing of RC3H2 inhibited OSCC cell proliferation, colony formation, migration, and invasion in vitro and reduced the expression of EZH2 and H3K27Me3, whereas RC3H2 overexpression significantly promoted OSCC cell growth, colony formation, migration, invasion, and xenograft tumor growth in vivo and increased the expression of EZH2 and H3K27Me3. A fluorescence in situ hybridization (FISH) assay verified that RC3H2 was predominately localized to the cytoplasm. RNA pull-down and luciferase activity assays showed that miR-101-3p was physically bound to RC3H2 as well as EZH2, and its inhibitor reversed the inhibitory effect of RC3H2 knockdown on progression of OSCC. Taken together, our findings demonstrate that RC3H2 as completive endogenous RNA sponging miR-101-3p targets EZH2 and facilitates OSCC cells' malignant behavior. CI - Copyright (c) 2020 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Wu, Kun AU - Wu K AD - Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. FAU - Jiang, Yingying AU - Jiang Y AD - Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Department of Dentistry, Affiliated Hospital, Weifang Medical University, Weifang 261031, China. FAU - Zhou, Wenkai AU - Zhou W AD - Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China. FAU - Zhang, Bolin AU - Zhang B AD - Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. FAU - Li, Yan AU - Li Y AD - Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. FAU - Xie, Fei AU - Xie F AD - Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. FAU - Zhang, Jianjun AU - Zhang J AD - Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China. FAU - Wang, Xu AU - Wang X AD - Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China. FAU - Yan, Ming AU - Yan M AD - Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China. FAU - Xu, Qin AU - Xu Q AD - Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China. FAU - Ren, Zhenhu AU - Ren Z AD - Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China. Electronic address: ren.zhenhu@outlook.com. FAU - Chen, Wantao AU - Chen W AD - Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. Electronic address: chenwantao196323@sjtu.edu.cn. FAU - Cao, Wei AU - Cao W AD - Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. Electronic address: caowei561521@shsmu.edu.cn. LA - eng PT - Journal Article DEP - 20200213 PL - United States TA - Mol Ther Nucleic Acids JT - Molecular therapy. Nucleic acids JID - 101581621 PMC - PMC7066035 OTO - NOTNLM OT - EZH2 OT - competitive endogenous RNA OT - invasion OT - lncRNA RC3H2 OT - metastasis OT - microRNA-101-3p OT - migration OT - oral squamous cell carcinoma OT - proliferation EDAT- 2020/03/13 06:00 MHDA- 2020/03/13 06:01 PMCR- 2020/02/13 CRDT- 2020/03/13 06:00 PHST- 2019/10/16 00:00 [received] PHST- 2020/01/25 00:00 [revised] PHST- 2020/02/05 00:00 [accepted] PHST- 2020/03/13 06:00 [pubmed] PHST- 2020/03/13 06:01 [medline] PHST- 2020/03/13 06:00 [entrez] PHST- 2020/02/13 00:00 [pmc-release] AID - S2162-2531(20)30078-0 [pii] AID - 10.1016/j.omtn.2020.02.006 [doi] PST - ppublish SO - Mol Ther Nucleic Acids. 2020 Jun 5;20:97-110. doi: 10.1016/j.omtn.2020.02.006. Epub 2020 Feb 13.