PMID- 32164368
OWN - NLM
STAT- MEDLINE
DCOM- 20201204
LR  - 20201214
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 5
DP  - 2020 Mar 10
TI  - Gene Polymorphisms in Boar Spermatozoa and Their Associations with Post-Thaw 
      Semen Quality.
LID - 10.3390/ijms21051902 [doi]
LID - 1902
AB  - Genetic markers have been used to assess the freezability of semen. With the 
      advancement in molecular genetic techniques, it is possible to assess the 
      relationships between sperm functions and gene polymorphisms. In this study, 
      variant calling analysis of RNA-Seq datasets was used to identify single 
      nucleotide polymorphisms (SNPs) in boar spermatozoa and to explore the 
      associations between SNPs and post-thaw semen quality. Assessment of post-thaw 
      sperm quality characteristics showed that 21 boars were considered as having good 
      semen freezability (GSF), while 19 boars were classified as having poor semen 
      freezability (PSF). Variant calling demonstrated that most of the polymorphisms 
      (67%) detected in boar spermatozoa were at the 3'-untranslated regions (3'-UTRs). 
      Analysis of SNP abundance in various functional gene categories showed that gene 
      ontology (GO) terms were related to response to stress, motility, metabolism, 
      reproduction, and embryo development. Genomic DNA was isolated from sperm samples 
      of 40 boars. Forty SNPs were selected and genotyped, and several SNPs were 
      significantly associated with motility and membrane integrity of frozen-thawed 
      (FT) spermatozoa. Polymorphism in SCLT1 gene was associated with significantly 
      higher motility and plasma membrane integrity of FT spermatozoa from boars of the 
      GSF group compared with those of the PSF group. Likewise, polymorphisms in 
      MAP3K20, MS4A2, and ROBO1 genes were significantly associated with reduced 
      cryo-induced lipid peroxidation and DNA damage of FT spermatozoa from boars of 
      the GSF group. Candidate genes with significant SNP associations, including 
      APPL1, PLBD1, FBXO16, EML5, RAB3C, OXSR1, PRICKLE1, and MAP3K20 genes, represent 
      potential markers for post-thaw semen quality, and they might be relevant for 
      future improvement in the selection procedure of boars for cryopreservation. The 
      findings of this study provide evidence indicating that polymorphisms in genes 
      expressed in spermatozoa could be considered as factors associated with post-thaw 
      semen quality.
FAU - Mankowska, Anna
AU  - Mankowska A
AD  - Department of Animal Biochemistry and Biotechnology, Faculty of Animal 
      Bioengineering, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, 
      Poland.
FAU - Brym, Pawel
AU  - Brym P
AD  - Department of Animal Genetics, Faculty of Animal Bioengineering, University of 
      Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.
FAU - Paukszto, Lukasz
AU  - Paukszto L
AUID- ORCID: 0000-0002-3618-1064
AD  - Department of Plant Physiology and Biotechnology, Faculty of Biology and 
      Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, 
      Poland.
FAU - Jastrzebski, Jan P
AU  - Jastrzebski JP
AUID- ORCID: 0000-0001-8699-7742
AD  - Department of Plant Physiology and Biotechnology, Faculty of Biology and 
      Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, 
      Poland.
FAU - Fraser, Leyland
AU  - Fraser L
AUID- ORCID: 0000-0002-2997-9991
AD  - Department of Animal Biochemistry and Biotechnology, Faculty of Animal 
      Bioengineering, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, 
      Poland.
LA  - eng
GR  - 2015/19/B/NZ9/01333/National Science Centre, Poland/
PT  - Journal Article
DEP - 20200310
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Cryopreservation
MH  - *Gene Regulatory Networks
MH  - Genetic Association Studies
MH  - *Genetic Markers
MH  - Male
MH  - *Polymorphism, Single Nucleotide
MH  - Semen/*chemistry
MH  - Semen Analysis/*veterinary
MH  - Semen Preservation
MH  - Sequence Analysis, RNA/veterinary
MH  - Swine
PMC - PMC7084667
OTO - NOTNLM
OT  - RNA-Seq
OT  - SNPs
OT  - freezability
OT  - genotype
OT  - variant calling
COIS- None of the authors have any conflicts of interest to declare.
EDAT- 2020/03/14 06:00
MHDA- 2020/12/15 06:00
PMCR- 2020/03/01
CRDT- 2020/03/14 06:00
PHST- 2020/02/01 00:00 [received]
PHST- 2020/03/04 00:00 [revised]
PHST- 2020/03/06 00:00 [accepted]
PHST- 2020/03/14 06:00 [entrez]
PHST- 2020/03/14 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/03/01 00:00 [pmc-release]
AID - ijms21051902 [pii]
AID - ijms-21-01902 [pii]
AID - 10.3390/ijms21051902 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Mar 10;21(5):1902. doi: 10.3390/ijms21051902.