PMID- 32165091
OWN - NLM
STAT- MEDLINE
DCOM- 20201027
LR  - 20201027
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 114
DP  - 2020 Jun
TI  - HIF-1alpha/SDF-1/CXCR4 axis reduces neuronal apoptosis via enhancing the bone 
      marrow-derived mesenchymal stromal cell migration in rats with traumatic brain 
      injury.
PG  - 104416
LID - S0014-4800(19)30847-0 [pii]
LID - 10.1016/j.yexmp.2020.104416 [doi]
AB  - Mesenchymal stromal injection is a promising therapy for traumatic brain injury 
      (TBI). The aim of this study was to explore the effects of the HIF-1alpha/SDF-1/CXCR4 
      axis on neuron repair in TBI rats through improving the bone marrow-derived 
      mesenchymalstromal cells (BMSCs) migration. TBI rat models were established. The 
      rats were treated with exogenous SDF-1, and then the neuronal apoptosis in TBI 
      rats was measured. BMSCs from rats were collected, and the roles of NF-kappaB p65 
      expression in nuclei, overexpression of SDF-1 and HIF-1alpha, as well as 
      downregulation of CXCR4 in BMSC migration were identified. HIF-1alpha- and SDF-1- 
      treated BMSCs were transplanted into TBI rats, after which the neuronal apoptosis 
      and activity of the HIF-1alpha/SDF-1/CXCR4 axis were detected. Consequently, we found 
      SDF-1 elevated the HIF-1alpha/SDF-1/CXCR4 activity and presented protective roles in 
      TBI rat hippocampal neurons with reduced neuronal apoptosis. SDF-1 promoted BMSC 
      migration in vitro, and co-effects of SDF-1 and HIF-1alpha showed strong promotion, 
      while CXCR4 inhibition suppressed BMSC migration. BMSC transplantation activated 
      the HIF-1alpha/SDF-1/CXCR4 axis and reduced neuronal apoptosis in TBI rats. To 
      conclude, our study demonstrated that the HIF-1alpha/SDF-1/CXCR4 axis could enhance 
      BMSC migration and alleviate neuronal damage and apoptosis in TBI rats. This 
      study provided novel options for TBI therapy.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Guo, Kai
AU  - Guo K
AD  - Department of Neurosurgery, Xingtai People's Hospital, Xingtai 054031, Hebei, PR 
      China.
FAU - Yao, Xinyu
AU  - Yao X
AD  - Department of Anesthesia, Xingtai People's Hospital, Xingtai 054031, Hebei, PR 
      China.
FAU - Wu, Weijing
AU  - Wu W
AD  - Department of Neurosurgery, Xingtai People's Hospital, Xingtai 054031, Hebei, PR 
      China.
FAU - Yu, Ziyi
AU  - Yu Z
AD  - Department of Intensive Care Unit,Tangshan Worker Hospital, Tangshan 063000, 
      Hebei, PR China.
FAU - Li, Zhenzhong
AU  - Li Z
AD  - Department of Neurosurgery, Xingtai People's Hospital, Xingtai 054031, Hebei, PR 
      China.
FAU - Ma, Zenglu
AU  - Ma Z
AD  - Department of Neurosurgery, Xingtai People's Hospital, Xingtai 054031, Hebei, PR 
      China.
FAU - Liu, Dengxiang
AU  - Liu D
AD  - Department of Radiotherapy, Xingtai People's Hospital, Xingtai 054031, Hebei, PR 
      China. Electronic address: Liudengx0709@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200309
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (CXCL12 protein, rat)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Cxcr4 protein, rat)
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Receptors, CXCR4)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - Brain Injuries, Traumatic/*genetics/metabolism/pathology
MH  - Cell Movement/genetics
MH  - Chemokine CXCL12/*genetics
MH  - Disease Models, Animal
MH  - Hippocampus/metabolism/pathology
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*genetics
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/metabolism/pathology
MH  - Neurons/metabolism/pathology
MH  - Rats
MH  - Receptors, CXCR4/*genetics
MH  - Signal Transduction/genetics
MH  - Stromal Cells/metabolism/pathology
OTO - NOTNLM
OT  - Bone marrow-derived mesenchymalstromal cells
OT  - Chemotaxis cytokine receptor-4
OT  - Hypoxia-inducible factor-1alpha
OT  - Neuronal apoptosis
OT  - Stromal cell-derived factor-l
OT  - Traumatic brain injury
COIS- Declaration of Competing Interest The authors declare that they have no conflict 
      of interest.
EDAT- 2020/03/14 06:00
MHDA- 2020/10/28 06:00
CRDT- 2020/03/14 06:00
PHST- 2019/11/07 00:00 [received]
PHST- 2020/02/21 00:00 [revised]
PHST- 2020/03/07 00:00 [accepted]
PHST- 2020/03/14 06:00 [pubmed]
PHST- 2020/10/28 06:00 [medline]
PHST- 2020/03/14 06:00 [entrez]
AID - S0014-4800(19)30847-0 [pii]
AID - 10.1016/j.yexmp.2020.104416 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2020 Jun;114:104416. doi: 10.1016/j.yexmp.2020.104416. Epub 2020 
      Mar 9.