PMID- 32165121
OWN - NLM
STAT- MEDLINE
DCOM- 20210831
LR  - 20210831
IS  - 1476-5411 (Electronic)
IS  - 1367-0484 (Linking)
VI  - 43
IP  - 6
DP  - 2020 Dec
TI  - Scleral lens wear: Measuring inflammation in the fluid reservoir.
PG  - 577-584
LID - S1367-0484(20)30035-7 [pii]
LID - 10.1016/j.clae.2020.02.017 [doi]
AB  - PURPOSE: To measure inflammatory mediators in the scleral lens fluid reservoir 
      (FR) in healthy eyes and to compare them to basal tear samples after 8-hs (8h) 
      and 4-days (4d) of scleral lens (SL) wear. METHODS: Fifteen normal, habitual soft 
      contact lens wearers were fitted with 14.8- or 15.4-mm SLs (Zenlens, Alden 
      Optical, USA). Basal ocular surface tears and FR samples were collected after 8h 
      and 4d of daily SL wear. Levels of interleukin (IL) -4 and -8, matrix 
      metalloproteinase (MMP)-7, -9, and -10, and tissue inhibitor of MMPs (TIMPs) 1-4 
      were measured in all samples using Luminex assays. Visual acuity, corneal and 
      conjunctival staining, and comfort assessments were completed at the baseline, 8h 
      and 4d time points. RESULTS: MMP-9 and MMP-10 were greater in FR than basal 
      ocular surface tears. After 8h of SL wear, the median concentration of MMP-9 in 
      the FR and basal tears were 62.7 and 15.2 ng/mL, respectively (p = 0.047). 
      Likewise, MMP-10 was significantly greater in FR compared to basal tears, after 
      8h (25.8 ng/mL vs 2.8 ng/mL, p < 0.001) and 4d (2.1 ng/mL vs17.2 ng/mL, p = 
      0.047). IL-4 and IL-8 levels were greater in FR but not significantly at 8h (2.2 
      vs 3.1 ng/mL; and 0.1 vs 0.4 ng/mL, respectively) or 4d (0.9 vs 3.5 ng/mL; 0.0 vs 
      0.2 ng/mL). MMP-7 was not affected by SL wear after 8h (46.0 basal vs 54.4 ng/mL 
      FR) or 4d (34.2 vs 87.5 ng/mL). Visual acuity, corneal and conjunctival staining 
      did not change; comfort was reduced in SL compared to soft contact lens wear. 
      CONCLUSIONS: This is the first study to compare the FR with the basal ocular 
      surface tears. MMP-9 and MMP-10 were elevated in the FR after several hours of SL 
      wear, suggesting potential clinical implications of SL wear and deserves further 
      investigation.
CI  - Copyright (c) 2020 British Contact Lens Association. Published by Elsevier Ltd. All 
      rights reserved.
FAU - Walker, Maria K
AU  - Walker MK
AD  - University of Houston College of Optometry, The Ocular Surface Institute, 4901 
      Calhoun Road, Houston, TX 77006, United States. Electronic address: 
      mkwalker@Central.UH.EDU.
FAU - Lema, Carolina
AU  - Lema C
AD  - University of Houston College of Optometry, The Ocular Surface Institute, 4901 
      Calhoun Road, Houston, TX 77006, United States. Electronic address: 
      fclemahe@Central.UH.EDU.
FAU - Redfern, Rachel
AU  - Redfern R
AD  - University of Houston College of Optometry, The Ocular Surface Institute, 4901 
      Calhoun Road, Houston, TX 77006, United States. Electronic address: 
      rredfer2@Central.UH.EDU.
LA  - eng
PT  - Journal Article
DEP - 20200309
PL  - England
TA  - Cont Lens Anterior Eye
JT  - Contact lens & anterior eye : the journal of the British Contact Lens Association
JID - 9712714
SB  - IM
MH  - *Contact Lenses, Hydrophilic
MH  - Cornea
MH  - Humans
MH  - Inflammation
MH  - *Sclera
MH  - Tears
OTO - NOTNLM
OT  - Fluid reservoir
OT  - MMP-10
OT  - MMP-9
OT  - Scleral lenses
EDAT- 2020/03/14 06:00
MHDA- 2021/09/01 06:00
CRDT- 2020/03/14 06:00
PHST- 2019/10/24 00:00 [received]
PHST- 2020/02/10 00:00 [revised]
PHST- 2020/02/25 00:00 [accepted]
PHST- 2020/03/14 06:00 [pubmed]
PHST- 2021/09/01 06:00 [medline]
PHST- 2020/03/14 06:00 [entrez]
AID - S1367-0484(20)30035-7 [pii]
AID - 10.1016/j.clae.2020.02.017 [doi]
PST - ppublish
SO  - Cont Lens Anterior Eye. 2020 Dec;43(6):577-584. doi: 10.1016/j.clae.2020.02.017. 
      Epub 2020 Mar 9.