PMID- 32167419
OWN - NLM
STAT- MEDLINE
DCOM- 20211227
LR  - 20211227
IS  - 1532-2378 (Electronic)
IS  - 1049-5398 (Linking)
VI  - 32
IP  - 5
DP  - 2021 Oct
TI  - OGR1 negatively regulates beta-casein and triglyceride synthesis and cell 
      proliferation via the PI3K/AKT/mTOR signaling pathway in goat mammary epithelial 
      cells.
PG  - 627-636
LID - 10.1080/10495398.2020.1737099 [doi]
AB  - Goat milk in some cases is less allergenic than cow milk, therefore, more people 
      drink goat milk in the world, so it is necessary for us to improve the yield and 
      quality of goat milk. Previous studies have shown that some genes are closely 
      related to lactation. Ovarian cancer G protein-coupled 1 (OGR1) is a G 
      protein-coupled receptor discovered recently. OGR1 is widely found in various 
      tissues of organisms and is involved in cell skeleton reorganization, 
      carcinogenesis, cell proliferation, and apoptosis by regulating multiple 
      signaling pathways in cells. However, the modulating effect of OGR1 in lactation 
      is still unknown. Therefore, the objective of this study is to investigate the 
      function of OGR1 in goat mammary epithelial cells (GMECs). Flow cytometry, CCK8, 
      EDU, enzyme-linked immunosorbent assay, and triglyceride test kit assays were 
      performed and we found that OGR1 regulated Bcl-2/Bax ratio, Fas protein 
      expression as well as the phosphorylation of AKT and mammalian target of 
      rapamycin (mTOR). si-OGR1 could enhance the proliferation of GMECs by promoting 
      G1/S phase progression and the synthesis of beta-casein and triglyceride. By 
      contrast, OGR1 repressed GMECs proliferation and down-regulated the synthesis of 
      beta-casein and triglyceride by blocking the PI3K/AKT/mTOR signaling pathway in 
      GMECs.
FAU - Zhu, Chao
AU  - Zhu C
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, P. R. China.
FAU - Wang, Lili
AU  - Wang L
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, P. 
      R. China.
FAU - Zhu, Junru
AU  - Zhu J
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, P. R. China.
FAU - Jiang, Yue
AU  - Jiang Y
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, P. R. China.
FAU - Du, Xiaoyan
AU  - Du X
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, P. R. China.
FAU - Duan, Quyu
AU  - Duan Q
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, P. R. China.
FAU - Yin, Hao
AU  - Yin H
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, P. R. China.
FAU - Huang, Xinrui
AU  - Huang X
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, P. R. China.
FAU - Song, Yuxuan
AU  - Song Y
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, P. R. China.
FAU - Cao, Binyun
AU  - Cao B
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, P. R. China.
FAU - Li, Guang
AU  - Li G
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, P. R. China.
FAU - An, Xiaopeng
AU  - An X
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20200313
PL  - England
TA  - Anim Biotechnol
JT  - Animal biotechnology
JID - 9011409
RN  - 0 (Caseins)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Triglycerides)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Caseins/*biosynthesis
MH  - Cell Proliferation
MH  - Epithelial Cells/metabolism
MH  - *Goats/genetics/metabolism
MH  - Phosphatidylinositol 3-Kinases/genetics
MH  - Proto-Oncogene Proteins c-akt/genetics
MH  - Receptors, G-Protein-Coupled/*genetics
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/genetics
MH  - Triglycerides/*biosynthesis
OTO - NOTNLM
OT  - Mammary epithelial cells
OT  - PI3K/AKT/mTOR signaling pathway
OT  - goat
OT  - proliferation
OT  - triglyceride
EDAT- 2020/03/14 06:00
MHDA- 2021/12/28 06:00
CRDT- 2020/03/14 06:00
PHST- 2020/03/14 06:00 [pubmed]
PHST- 2021/12/28 06:00 [medline]
PHST- 2020/03/14 06:00 [entrez]
AID - 10.1080/10495398.2020.1737099 [doi]
PST - ppublish
SO  - Anim Biotechnol. 2021 Oct;32(5):627-636. doi: 10.1080/10495398.2020.1737099. Epub 
      2020 Mar 13.