PMID- 32168750
OWN - NLM
STAT- MEDLINE
DCOM- 20210316
LR  - 20210316
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 9
IP  - 3
DP  - 2020 Mar 11
TI  - 3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human 
      Neuroblastoma Cells.
LID - 10.3390/cells9030682 [doi]
LID - 682
AB  - We prepared cellulose nanofibrils-based (CNF), alginate-based and single-walled 
      carbon nanotubes (SWCNT)-based inks for freeform reversible embedding hydrogel 
      (FRESH) 3D bioprinting of conductive scaffolds. The 3D printability of conductive 
      inks was evaluated in terms of their rheological properties. The differentiation 
      of human neuroblastoma cells (SH-SY5Y cell line) was visualized by the confocal 
      microscopy and the scanning electron microscopy techniques. The expression of 
      TUBB3 and Nestin genes was monitored by the RT-qPCR technique. We have 
      demonstrated that the conductive guidelines promote the cell differentiation, 
      regardless of using differentiation factors. It was also shown that the 
      electrical conductivity of the 3D printed scaffolds could be tuned by 
      calcium-induced crosslinking of alginate, and this plays a significant role on 
      neural cell differentiation. Our work provides a protocol for the generation of a 
      realistic in vitro 3D neural model and allows for a better understanding of the 
      pathological mechanisms of neurodegenerative diseases.
FAU - Bordoni, Matteo
AU  - Bordoni M
AD  - Genomic and post-Genomic Center, IRCCS Mondino Foundation, Via Mondino 2, 27100 
      Pavia, Italy.
FAU - Karabulut, Erdem
AU  - Karabulut E
AUID- ORCID: 0000-0002-7878-5878
AD  - 3D Bioprinting Center, Chalmers University of Technology, Arvid Wallgrens backe 
      20, 41346 Goteborg, Sweden.
AD  - Wallenberg Wood Science Center, Arvid Wallgrens backe 20, 41346 Goteborg, Sweden.
FAU - Kuzmenko, Volodymyr
AU  - Kuzmenko V
AD  - Wallenberg Wood Science Center, Arvid Wallgrens backe 20, 41346 Goteborg, Sweden.
AD  - Department of Microtechnology and Nanoscience, Chalmers University of Technology, 
      Kemivagen 9, 41258 Goteborg, Sweden.
FAU - Fantini, Valentina
AU  - Fantini V
AUID- ORCID: 0000-0003-1244-4453
AD  - Department of Brain and Behavioural Sciences, University of Pavia, Via Forlanini 
      6, 27100 Pavia, Italy.
AD  - Laboratory of Neurobiology and Neurogenetic, Golgi-Cenci Foundation, Corso S. 
      Martino 10, 20081 Abbiategrasso, Milan, Italy.
FAU - Pansarasa, Orietta
AU  - Pansarasa O
AD  - Genomic and post-Genomic Center, IRCCS Mondino Foundation, Via Mondino 2, 27100 
      Pavia, Italy.
FAU - Cereda, Cristina
AU  - Cereda C
AD  - Genomic and post-Genomic Center, IRCCS Mondino Foundation, Via Mondino 2, 27100 
      Pavia, Italy.
FAU - Gatenholm, Paul
AU  - Gatenholm P
AD  - 3D Bioprinting Center, Chalmers University of Technology, Arvid Wallgrens backe 
      20, 41346 Goteborg, Sweden.
AD  - Wallenberg Wood Science Center, Arvid Wallgrens backe 20, 41346 Goteborg, Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200311
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Nanotubes, Carbon)
RN  - 9004-34-6 (Cellulose)
SB  - IM
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cellulose/*pharmacology
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/drug effects
MH  - *Nanotubes, Carbon/adverse effects
MH  - Neuroblastoma/*pathology
MH  - *Printing, Three-Dimensional
MH  - Tissue Scaffolds
PMC - PMC7140699
OTO - NOTNLM
OT  - 3D bioprinting
OT  - 3D cell cultures
OT  - carbon nanotubes
OT  - cellular models
OT  - conductive scaffold
COIS- The authors declare no conflict of interest.
EDAT- 2020/03/15 06:00
MHDA- 2021/03/17 06:00
PMCR- 2020/03/01
CRDT- 2020/03/15 06:00
PHST- 2020/01/31 00:00 [received]
PHST- 2020/03/06 00:00 [revised]
PHST- 2020/03/07 00:00 [accepted]
PHST- 2020/03/15 06:00 [entrez]
PHST- 2020/03/15 06:00 [pubmed]
PHST- 2021/03/17 06:00 [medline]
PHST- 2020/03/01 00:00 [pmc-release]
AID - cells9030682 [pii]
AID - cells-09-00682 [pii]
AID - 10.3390/cells9030682 [doi]
PST - epublish
SO  - Cells. 2020 Mar 11;9(3):682. doi: 10.3390/cells9030682.