PMID- 32169023
OWN - NLM
STAT- MEDLINE
DCOM- 20210819
LR  - 20231213
IS  - 1521-0499 (Electronic)
IS  - 0190-2148 (Linking)
VI  - 46
IP  - 5
DP  - 2020 May-Jun
TI  - The expression of beta-Defensin-2, IL-22, IL-22R1 and IL-10R2 in rat model of 
      Klebsiella pneumonia and their correlation with histological grades.
PG  - 109-116
LID - 10.1080/01902148.2020.1725690 [doi]
AB  - Backgrounds and Aims:Klebsiella pneumoniae represents the most common 
      opportunistic pathogen contributing to Klebsiella pneumonia in hospital-acquired 
      infections. Klebsiella pneumonia has a rapidly progressive clinical course and 
      multi-drug resistant (MDR). Identification of the effective biochemical markers 
      is crucial for improving early diagnosis and treatment of Klebsiella pneumonia. 
      The aims of our study are to 1) investigate the expression of beta-Defensin-2(rbetaD2), 
      IL-22, IL-22R1 and IL-10R2 in Klebsiella pneumonia-infected rats and 2) their 
      association with the histological grades of Klebsiella pneumonia.Methods and 
      Materials: Fifty specific pathogen free (SPF) male SD rats were randomly divided 
      into two groups: control group (treated with normal saline) and pneumonia group 
      (treated with K. pneumoniae). All animals were sacrificed 1 h, 12 h, 1 d, 3 d, 5 
      d post infection. The severity and property of pneumonia was evaluated by 
      histopathologic observation and pathogen identification. The mRNA expression of 
      rbetaD2, IL-22, IL-22R1 and IL-10R2 was measured by RT-qPCR assay. The expression of 
      rbetaD2 in rat lung tissue was determined by Western blot analysis, and the level of 
      IL-22 in rat serum was determined by ELISA.Results: Histopathologic examination 
      and bacterial counting of lung tissues confirmed the successful establishment of 
      rat pneumonia model. The gene expression of rbetaD2, IL-22, IL-22R1 and IL-10R2 in 
      pneumonia rats were significantly higher than those in healthy control mice 
      (P < 0.05). The expression of rbetaD2 was correlated with histological grades of 
      Klebsiella pneumonia and the level of IL-22. RT-qPCR results showed that the peak 
      expression of IL-22R1 appeared earlier than IL-10R2 in rat pneumonia 
      model.Conclusions: The expression of rbetaD2 and IL-22 was increased significantly 
      at early stage in rat Klebsiella pneumonia model, suggesting that IL-22 and rbetaD2 
      might serve as potential biomarkers for the early diagnosis of Klebsiella 
      pneumonia.
FAU - Fan, Jianyong
AU  - Fan J
AD  - Department of Respiratory Diseases, Xi'an International Medical Center, Xi'an, 
      Shanxi Province, China.
FAU - Luo, Yuan
AU  - Luo Y
AD  - Department of Respiratory Diseases, Hefei Second People's Hospital, Hefei, Anhui 
      Province, China.
FAU - Qin, Yan
AU  - Qin Y
AD  - Department of Geriatrics Diseases, Xi'an 521 Hospital, Xi'an, Shanxi Province, 
      China.
FAU - Wu, Changgui
AU  - Wu C
AD  - Department of Respiratory Diseases, Xi'an International Medical Center, Xi'an, 
      Shanxi Province, China.
FAU - Han, Xinpeng
AU  - Han X
AD  - Department of Respiratory Diseases, Xi'an International Medical Center, Xi'an, 
      Shanxi Province, China.
FAU - Ouyang, Haifeng
AU  - Ouyang H
AD  - Department of Respiratory Diseases, Xi'an International Medical Center, Xi'an, 
      Shanxi Province, China.
FAU - Zhang, Liyuan
AU  - Zhang L
AD  - Department of Respiratory Diseases, Xi'an International Medical Center, Xi'an, 
      Shanxi Province, China.
FAU - Cai, Pei
AU  - Cai P
AD  - Department of Respiratory Diseases, Xi'an International Medical Center, Xi'an, 
      Shanxi Province, China.
FAU - Li, Nie
AU  - Li N
AD  - Department of Respiratory Diseases, Xi'an International Medical Center, Xi'an, 
      Shanxi Province, China.
LA  - eng
PT  - Journal Article
DEP - 20200313
PL  - England
TA  - Exp Lung Res
JT  - Experimental lung research
JID - 8004944
RN  - 0 (Interleukin-10 Receptor beta Subunit)
RN  - 0 (Interleukins)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (beta-Defensins)
RN  - 0 (interleukin-22 receptor)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Interleukin-10 Receptor beta Subunit/*metabolism
MH  - Interleukins/*metabolism
MH  - Klebsiella Infections/*metabolism/pathology
MH  - Klebsiella pneumoniae
MH  - Lung/*pathology
MH  - Male
MH  - Rats, Sprague-Dawley
MH  - Receptors, Interleukin/metabolism
MH  - beta-Defensins/*metabolism
MH  - Interleukin-22
OTO - NOTNLM
OT  - Beta-defensin-2
OT  - IL-10R2
OT  - IL-22
OT  - IL-22R1
OT  - Klebsiella pneumoniae
OT  - pneumonia
EDAT- 2020/03/15 06:00
MHDA- 2021/08/20 06:00
CRDT- 2020/03/15 06:00
PHST- 2020/03/15 06:00 [pubmed]
PHST- 2021/08/20 06:00 [medline]
PHST- 2020/03/15 06:00 [entrez]
AID - 10.1080/01902148.2020.1725690 [doi]
PST - ppublish
SO  - Exp Lung Res. 2020 May-Jun;46(5):109-116. doi: 10.1080/01902148.2020.1725690. 
      Epub 2020 Mar 13.