PMID- 32170198
OWN - NLM
STAT- MEDLINE
DCOM- 20201123
LR  - 20210313
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Mar 13
TI  - The role of Ca(2+)/NFAT in Dysfunction and Inflammation of Human Coronary 
      Endothelial Cells induced by Sera from patients with Kawasaki disease.
PG  - 4706
LID - 10.1038/s41598-020-61667-y [doi]
LID - 4706
AB  - Ca(2+)/nuclear factor of activated T-cells (Ca(2+)/NFAT) signaling pathway may 
      play a crucial role in the pathogenesis of Kawasaki disease (KD). We investigated 
      the poorly understood Ca(2+)/NFAT regulation of coronary artery endothelial cells 
      and consequent dysfunction in KD pathogenesis. Human coronary artery endothelial 
      cells (HCAECs) stimulated with sera from patients with KD, compared with sera 
      from healthy children, exhibited significant increases in proliferation and 
      angiogenesis, higher levels of NFATc1 and NFATc3 and some inflammatory molecules, 
      and increased nuclear translocation of NFATc1 and NFATc3. HCAECs stimulated with 
      sera from patients with KD treated with cyclosporine A (CsA) showed decreased 
      proliferation, angiogenesis, NFATc1 and inflammatory molecules levels as compared 
      with results for untreated HCAECs. In conclusion, our data reveal that KD sera 
      activate the Ca(2+)/NFAT in HCAECs, leading to dysfunction and inflammation of 
      endothelial cells. CsA has cytoprotective effects by ameliorating endothelial 
      cell homeostasis via Ca(2+)/NFAT.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Cardiology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, No. 3333 Binsheng 
      Road, Hangzhou, 310052, PR China.
FAU - Hu, Jian
AU  - Hu J
AD  - Department of Cardiology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, No. 3333 Binsheng 
      Road, Hangzhou, 310052, PR China.
FAU - Liu, Jingjing
AU  - Liu J
AD  - Department of Cardiology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, No. 3333 Binsheng 
      Road, Hangzhou, 310052, PR China.
FAU - Geng, Zhimin
AU  - Geng Z
AD  - Department of Cardiology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, No. 3333 Binsheng 
      Road, Hangzhou, 310052, PR China.
FAU - Tao, Yijing
AU  - Tao Y
AD  - Department of Cardiology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, No. 3333 Binsheng 
      Road, Hangzhou, 310052, PR China.
FAU - Zheng, Fenglei
AU  - Zheng F
AD  - Department of Cardiology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, No. 3333 Binsheng 
      Road, Hangzhou, 310052, PR China.
FAU - Wang, Yujia
AU  - Wang Y
AD  - Department of Cardiology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, No. 3333 Binsheng 
      Road, Hangzhou, 310052, PR China.
FAU - Fu, Songling
AU  - Fu S
AD  - Department of Cardiology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, No. 3333 Binsheng 
      Road, Hangzhou, 310052, PR China.
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Cardiology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, No. 3333 Binsheng 
      Road, Hangzhou, 310052, PR China.
FAU - Xie, Chunhong
AU  - Xie C
AD  - Department of Cardiology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, No. 3333 Binsheng 
      Road, Hangzhou, 310052, PR China.
FAU - Zhang, Yiying
AU  - Zhang Y
AD  - Department of Cardiology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, No. 3333 Binsheng 
      Road, Hangzhou, 310052, PR China.
FAU - Gong, Fangqi
AU  - Gong F
AD  - Department of Cardiology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, No. 3333 Binsheng 
      Road, Hangzhou, 310052, PR China. gongfangqi@zju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200313
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (ILF3 protein, human)
RN  - 0 (Nuclear Factor 90 Proteins)
RN  - 0 (RNA, Messenger)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*metabolism
MH  - *Disease Susceptibility
MH  - Endothelial Cells/*metabolism
MH  - Gene Expression
MH  - Homeostasis/genetics
MH  - Humans
MH  - Mucocutaneous Lymph Node Syndrome/*etiology/*metabolism/pathology
MH  - Nuclear Factor 90 Proteins/*metabolism
MH  - RNA, Messenger
PMC - PMC7069934
COIS- The authors declare no competing interests.
EDAT- 2020/03/15 06:00
MHDA- 2020/11/24 06:00
PMCR- 2020/03/13
CRDT- 2020/03/15 06:00
PHST- 2019/11/20 00:00 [received]
PHST- 2020/02/26 00:00 [accepted]
PHST- 2020/03/15 06:00 [entrez]
PHST- 2020/03/15 06:00 [pubmed]
PHST- 2020/11/24 06:00 [medline]
PHST- 2020/03/13 00:00 [pmc-release]
AID - 10.1038/s41598-020-61667-y [pii]
AID - 61667 [pii]
AID - 10.1038/s41598-020-61667-y [doi]
PST - epublish
SO  - Sci Rep. 2020 Mar 13;10(1):4706. doi: 10.1038/s41598-020-61667-y.