PMID- 32170381
OWN - NLM
STAT- MEDLINE
DCOM- 20210108
LR  - 20210108
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 412
IP  - 12
DP  - 2020 May
TI  - Rapid and sensitive analysis of progesterone by solid-phase extraction with 
      amino-functionalized metal-organic frameworks coupled to direct analysis in 
      real-time mass spectrometry.
PG  - 2939-2947
LID - 10.1007/s00216-020-02535-6 [doi]
AB  - Progesterone is the representative progestogens in five major classes of steroid 
      hormones and plays important roles in mammalian pregnancy and animal growth and 
      development. Conventional available analytical methods for progesterone involve 
      immunoassay, gas chromatography-mass spectrometry (GC-MS), and high-performance 
      liquid chromatography-mass spectrometry (HPLC-MS), which lack specificity or 
      usually require sophisticated operations and relatively long time. Herein, we 
      developed a novel strategy for rapid analysis of progesterone via direct analysis 
      in real time mass spectrometry (DART-MS) combined with solid-phase extraction 
      (SPE) using an amino functionalized metal-organic frameworks (MOFs). Under 
      optimized conditions, a wide linear range of 0.5-500 ng mL(-1) was achieved, with 
      a satisfactory correlation coefficient (R(2) = 0.9992). The relative standard 
      deviations (RSDs) were in the range from 2.4 to 8.4%, demonstrating good 
      precision. The applicability was then confirmed by analyzing spiked lake water 
      and synthetic urine samples, and recoveries are between 92.0 and 117.8% in all 
      three spiked levels (5, 25, and 100 ng mL(-1)). The sensitivity was notably 
      improved compared with solely DART-MS and obtained detection limit decreased by 
      about 50 times. This research provided a rapid, simple, highly sensitive, and 
      efficient approach for analysis of hormones through combination of advantages of 
      ambient mass spectrometry and porous nanomaterials. Graphical abstract.
FAU - Li, Linnan
AU  - Li L
AD  - The MOE Key Laboratory of Standardization of Chinese Medicines, The SATCM Key 
      Laboratory of New Resources and Quality Evaluation of Chinese Medicines, 
      Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese 
      Medicine, Shanghai, 201203, China.
FAU - Chen, Yilin
AU  - Chen Y
AD  - The MOE Key Laboratory of Standardization of Chinese Medicines, The SATCM Key 
      Laboratory of New Resources and Quality Evaluation of Chinese Medicines, 
      Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese 
      Medicine, Shanghai, 201203, China.
FAU - Yang, Yuangui
AU  - Yang Y
AD  - The MOE Key Laboratory of Standardization of Chinese Medicines, The SATCM Key 
      Laboratory of New Resources and Quality Evaluation of Chinese Medicines, 
      Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese 
      Medicine, Shanghai, 201203, China.
FAU - Yang, Ying
AU  - Yang Y
AD  - The MOE Key Laboratory of Standardization of Chinese Medicines, The SATCM Key 
      Laboratory of New Resources and Quality Evaluation of Chinese Medicines, 
      Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese 
      Medicine, Shanghai, 201203, China.
FAU - Yang, Li
AU  - Yang L
AD  - The MOE Key Laboratory of Standardization of Chinese Medicines, The SATCM Key 
      Laboratory of New Resources and Quality Evaluation of Chinese Medicines, 
      Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese 
      Medicine, Shanghai, 201203, China. yl7@shutcm.edu.cn.
AD  - Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai, 201203, 
      China. yl7@shutcm.edu.cn.
FAU - Wang, Zhengtao
AU  - Wang Z
AD  - The MOE Key Laboratory of Standardization of Chinese Medicines, The SATCM Key 
      Laboratory of New Resources and Quality Evaluation of Chinese Medicines, 
      Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese 
      Medicine, Shanghai, 201203, China. ztwang@shutcm.edu.cn.
AD  - Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai, 201203, 
      China. ztwang@shutcm.edu.cn.
LA  - eng
GR  - 81573581/National Natural Science Foundation of China/
GR  - 2019M661597/China Postdoctoral Science Foundation/
GR  - 19YF1448800/Shanghai Sailing Program/
PT  - Journal Article
DEP - 20200313
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Metal-Organic Frameworks)
RN  - 0 (Water Pollutants, Chemical)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Humans
MH  - Lakes
MH  - Limit of Detection
MH  - Metal-Organic Frameworks/*chemistry
MH  - Progesterone/*analysis
MH  - Solid Phase Extraction/*methods
MH  - Tandem Mass Spectrometry/*methods
MH  - Urine/*chemistry
MH  - Water Pollutants, Chemical/*analysis
OTO - NOTNLM
OT  - Direct analysis in real time mass spectrometry
OT  - Metal-organic frameworks
OT  - Progesterone
OT  - Sample preparation
OT  - Solid-phase extraction
OT  - Steroid hormones
EDAT- 2020/03/15 06:00
MHDA- 2021/01/09 06:00
CRDT- 2020/03/15 06:00
PHST- 2019/12/30 00:00 [received]
PHST- 2020/02/18 00:00 [accepted]
PHST- 2020/02/12 00:00 [revised]
PHST- 2020/03/15 06:00 [pubmed]
PHST- 2021/01/09 06:00 [medline]
PHST- 2020/03/15 06:00 [entrez]
AID - 10.1007/s00216-020-02535-6 [pii]
AID - 10.1007/s00216-020-02535-6 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2020 May;412(12):2939-2947. doi: 10.1007/s00216-020-02535-6. 
      Epub 2020 Mar 13.