PMID- 32170785
OWN - NLM
STAT- MEDLINE
DCOM- 20210622
LR  - 20210622
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Linking)
VI  - 60
IP  - 7
DP  - 2020 Jul
TI  - Thrombocyte apheresis cassettes as a novel source of viable peripheral blood 
      mononuclear cells.
PG  - 1500-1507
LID - 10.1111/trf.15756 [doi]
AB  - BACKGROUND: Traditionally, white blood cells (WBCs) are collected from buffy 
      coats or freshly drawn blood. However, the increasing demand for peripheral blood 
      mononuclear cells (PBMCs) in the research phases of immunological therapy 
      development makes it necessary to identify alternative sources of these cells. 
      STUDY DESIGN AND METHODS: Leukapheresis products are cost intensive and not 
      offered by all blood banks. Therefore, thrombocyte apheresis cassettes (TACs), 
      plateletpheresis waste products, were investigated as a possible low-cost and 
      easily accessible blood source for research laboratories. The recovery rate, 
      phenotype, and functionality of WBC subsets from TAC are unknown and were 
      investigated in comparison to frequently used blood resources via flow cytometry. 
      RESULTS: On average, TACs provide 30.3 x 10(6) /mL PBMCs, situating themselves 
      between peripheral whole blood (WB; 5.35 x 10(6) /mL) and leukoreduction system 
      chamber (LRSC; 163.9 x 10(6) /mL) yields. Frequencies of CD14, CD3, CD4, CD8, 
      CD56, CD19, and CD11c positive cells in TACs correlate with normal proportions of 
      WBC populations. Stimulation of TAC-derived PBMCs by lipopolysaccharide (LPS) and 
      resiquimod (R848) showed no significant differences in expression levels of human 
      leukocyte antigen (HLA)-DR, DQ, DP, and CD86 or cytokine secretion compared to 
      other blood source derived PBMC. Following stimulation with LPS or R848, 
      comparable levels of tumor necrosis factor-alpha, interleukin-10, and interleukin-1beta 
      could be measured between TAC, LRSC, and WB. Additionally, TAC-derived T cells 
      retained their proliferation capability and were able to produce interferon-gamma 
      following T-cell receptor stimulation. CONCLUSION: TACs provide a cost-effective 
      source of viable and functional human blood cells that can readily be used for 
      clinical and laboratory investigations after plateletpheresis preparation.
CI  - (c) 2020 The Authors. Transfusion published by Wiley Periodicals, Inc. on behalf of 
      AABB.
FAU - Cunningham, Sarah
AU  - Cunningham S
AUID- ORCID: 0000-0001-8692-1211
AD  - Department of Transfusion Medicine and Hemostaseology, University Hospital 
      Erlangen, Erlangen, Germany.
FAU - Buchele, Vera
AU  - Buchele V
AD  - Department of Transfusion Medicine and Hemostaseology, University Hospital 
      Erlangen, Erlangen, Germany.
FAU - Brox, Regine
AU  - Brox R
AD  - Department of Transfusion Medicine and Hemostaseology, University Hospital 
      Erlangen, Erlangen, Germany.
FAU - Strasser, Erwin
AU  - Strasser E
AD  - Department of Transfusion Medicine and Hemostaseology, University Hospital 
      Erlangen, Erlangen, Germany.
FAU - Hackstein, Holger
AU  - Hackstein H
AUID- ORCID: 0000-0001-9722-8180
AD  - Department of Transfusion Medicine and Hemostaseology, University Hospital 
      Erlangen, Erlangen, Germany.
LA  - eng
PT  - Journal Article
DEP - 20200314
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Cytokines)
SB  - IM
MH  - *Blood Platelets/cytology/metabolism
MH  - Cytokines/blood
MH  - Female
MH  - *Flow Cytometry
MH  - Humans
MH  - *Leukocyte Reduction Procedures
MH  - *Leukocytes, Mononuclear/cytology/metabolism
MH  - Male
MH  - *Plateletpheresis
EDAT- 2020/03/15 06:00
MHDA- 2021/06/23 06:00
CRDT- 2020/03/15 06:00
PHST- 2019/12/04 00:00 [received]
PHST- 2020/02/14 00:00 [revised]
PHST- 2020/02/14 00:00 [accepted]
PHST- 2020/03/15 06:00 [pubmed]
PHST- 2021/06/23 06:00 [medline]
PHST- 2020/03/15 06:00 [entrez]
AID - 10.1111/trf.15756 [doi]
PST - ppublish
SO  - Transfusion. 2020 Jul;60(7):1500-1507. doi: 10.1111/trf.15756. Epub 2020 Mar 14.