PMID- 32173451
OWN - NLM
STAT- MEDLINE
DCOM- 20201223
LR  - 20201223
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 100
DP  - 2020 May
TI  - Bacteria-induced IL-1beta and its receptors in snakehead (Channa argus): Evidence 
      for their involvement in antibacterial innate immunity.
PG  - 309-316
LID - S1050-4648(20)30190-X [pii]
LID - 10.1016/j.fsi.2020.03.022 [doi]
AB  - As a central pro-inflammatory cytokine, interleukin-1beta (IL-1beta) plays critical 
      roles in the inflammatory response, pathogen infection, and immunological 
      challenges in mammals. Although fish IL-1beta has been confirmed to participate in 
      inflammatory response to pathogen infection, few studies have been performed to 
      characterize the antibacterial and bactericidal functions of fish IL-1beta. In this 
      study, snakehead (Channa argus) IL-1beta (shIL-1beta) and its receptors, shIL-1R1 and 
      shIL-1R2, were cloned and functionally characterized. ShIL-1beta contained the IL-1 
      family signature domain, and a potential cutting site at Asp(96) that presented 
      in all vertebrate IL-1beta sequences. ShIL-1R1 had three extracellular IG-like 
      domains and one intracellular signal TIR domain, while shIL-1R2 had three 
      extracellular IG-like domain but lacked the intracellular signal TIR domain. 
      ShIL-1beta, shIL-1R1, and shIL-1R2 were constitutively expressed in all tested 
      tissues, and their expressions could be induced by Aeromonas schubertii and 
      Nocardia seriolae in the head kidney and spleen in vivo, and by LTA, LPS, and 
      Poly (I:C) in head kidney leukocytes (HKLs) in vitro. Moreover, recombinant 
      shIL-1beta upregulated the expression of endogenous shIL-1beta, shIL-R1, and shIL-R2 in 
      snakehead HKLs, and enhanced intracellular bactericidal activity. Taken together, 
      this study found that, like IL-1beta and its receptors in mammals, shIL-1beta and its 
      receptors play crucial roles in antibacterial innate immunity. This provides new 
      insight into the evolution of IL-1beta function in vertebrates.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Cui, Zheng-Wei
AU  - Cui ZW
AD  - Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural 
      Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong 
      Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Guangzhou, China.
FAU - Kong, Lu-Lu
AU  - Kong LL
AD  - Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural 
      Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong 
      Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Guangzhou, China; College of Fisheries and Life Science, Shanghai Ocean 
      University, Shanghai, China.
FAU - Zhao, Fei
AU  - Zhao F
AD  - Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural 
      Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong 
      Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Guangzhou, China. Electronic address: zhaofei@prfri.ac.cn.
FAU - Tan, Ai-Ping
AU  - Tan AP
AD  - Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural 
      Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong 
      Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Guangzhou, China.
FAU - Deng, Yu-Ting
AU  - Deng YT
AD  - Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural 
      Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong 
      Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Guangzhou, China.
FAU - Jiang, Lan
AU  - Jiang L
AD  - Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural 
      Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong 
      Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20200312
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Receptors, Interleukin-1)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents
MH  - Bacteria/*immunology
MH  - Bacterial Infections/immunology/*veterinary
MH  - Carps/genetics/*immunology/microbiology
MH  - Cloning, Molecular
MH  - Fish Diseases/*immunology/microbiology
MH  - Head Kidney/immunology
MH  - *Immunity, Innate
MH  - Interleukin-1beta/*genetics/immunology
MH  - Receptors, Interleukin-1/*genetics/immunology
OTO - NOTNLM
OT  - Aeromonas schubertii
OT  - Antibacterial immunity
OT  - IL-1beta
OT  - Nocardia seriolae
OT  - Snakehead
COIS- Declaration of competing interest The authors have no conflicting commercial or 
      financial interest in publishing this paper.
EDAT- 2020/03/17 06:00
MHDA- 2020/12/29 06:00
CRDT- 2020/03/17 06:00
PHST- 2020/01/24 00:00 [received]
PHST- 2020/03/10 00:00 [revised]
PHST- 2020/03/11 00:00 [accepted]
PHST- 2020/03/17 06:00 [pubmed]
PHST- 2020/12/29 06:00 [medline]
PHST- 2020/03/17 06:00 [entrez]
AID - S1050-4648(20)30190-X [pii]
AID - 10.1016/j.fsi.2020.03.022 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2020 May;100:309-316. doi: 10.1016/j.fsi.2020.03.022. 
      Epub 2020 Mar 12.