PMID- 32174109 OWN - NLM STAT- MEDLINE DCOM- 20210106 LR - 20210106 IS - 1944-8252 (Electronic) IS - 1944-8244 (Linking) VI - 12 IP - 14 DP - 2020 Apr 8 TI - Selection of Affinity Reagents to Neutralize the Hemolytic Toxicity of Melittin Based on a Self-Assembled Nanoparticle Library. PG - 16040-16049 LID - 10.1021/acsami.0c00303 [doi] AB - Antibodies are the most common affinity reagents for specific target recognition. However, their applications are limited by high cost and low stability. Thus, seeking substitutes for antibodies is of great significance. In this work, we designed a library containing 82 self-assembled nanoparticles (SNPs) based on the self-assembly of beta-cyclodextrin polymers and adamantane derivatives, and then screened out eight types of SNPs capable of suppressing the toxicity of melittin using a hemolytic activity neutralization assay. The affinities of the SNPs to melittin were demonstrated using surface plasmon resonance (SPR). As evidenced by cytotoxicity experiments, SNPs could also suppress the toxicity of melittin to other cells. In addition, to verify the universality of our method, 11 types of SNPs capable of neutralizing another toxic peptide, phenolic soluble polypeptide (PSMalpha3) secreted by Staphylococcus aureus, were selected from the same SNP library. Our self-assembly-based method for the library preparation has the advantages of flexible design, mild experimental condition, and simple operation, which is expected to seek artificial affinity reagents for more species. FAU - Xu, Yaqing AU - Xu Y AD - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. FAU - Deng, Meitao AU - Deng M AD - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. FAU - Zhang, Haitao AU - Zhang H AD - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. FAU - Tan, Sha AU - Tan S AD - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. FAU - Li, Dan AU - Li D AD - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. FAU - Li, Shaoyuan AU - Li S AD - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. FAU - Luo, Lei AU - Luo L AD - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. FAU - Liao, Guofu AU - Liao G AD - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. FAU - Wang, Qing AU - Wang Q AD - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. FAU - Huang, Jin AU - Huang J AUID- ORCID: 0000-0002-2890-682X AD - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. FAU - Liu, Jianbo AU - Liu J AD - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. FAU - Yang, Xiaohai AU - Yang X AUID- ORCID: 0000-0001-8122-7140 AD - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. FAU - Wang, Kemin AU - Wang K AUID- ORCID: 0000-0001-9390-4938 AD - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. LA - eng PT - Journal Article DEP - 20200326 PL - United States TA - ACS Appl Mater Interfaces JT - ACS applied materials & interfaces JID - 101504991 RN - 0 (Antibodies) RN - 0 (Polymers) RN - 0 (beta-Cyclodextrins) RN - 20449-79-0 (Melitten) RN - JV039JZZ3A (betadex) SB - IM MH - Antibodies/chemistry/*immunology MH - Cytotoxicity Tests, Immunologic MH - Humans MH - Melitten/*chemistry MH - Nanoparticles/*chemistry MH - Polymers/chemistry MH - Staphylococcus aureus/*chemistry MH - Surface Plasmon Resonance MH - beta-Cyclodextrins/chemistry OTO - NOTNLM OT - affinity reagent OT - hemolytic toxicity OT - melittin OT - self-assembled nanoparticle OT - beta-cyclodextrin polymer EDAT- 2020/03/17 06:00 MHDA- 2021/01/07 06:00 CRDT- 2020/03/17 06:00 PHST- 2020/03/17 06:00 [pubmed] PHST- 2021/01/07 06:00 [medline] PHST- 2020/03/17 06:00 [entrez] AID - 10.1021/acsami.0c00303 [doi] PST - ppublish SO - ACS Appl Mater Interfaces. 2020 Apr 8;12(14):16040-16049. doi: 10.1021/acsami.0c00303. Epub 2020 Mar 26.