PMID- 32175085 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220413 IS - 2055-6640 (Print) IS - 2055-6659 (Electronic) IS - 2055-6640 (Linking) VI - 6 IP - 1 DP - 2020 Feb 20 TI - Feasibility and safety of research sigmoid colon biopsy in a cohort of Thai men who have sex with men with acute HIV-1. PG - 7-10 AB - BACKGROUND: The gut-associated lymphoid tissue (GALT) is a major reservoir of HIV-1 established early in acute HIV-1 infection (AHI). Sampling tissue from GALT can provide information about viral reservoirs and immune responses but may be complicated during AHI for reasons such as high viral replication, CD4 T cell depletion and immune activation. Risk of adverse events (AEs) associated with research sigmoid colon biopsies was assessed in participants with AHI in Bangkok, Thailand. METHODS: Between 2009 and 2016, 170 biopsies collected from the sigmoid colon were performed during AHI and at follow-up visits (median 24 weeks post AHI diagnosis). Adverse event incidence was evaluated, as well as the associations of procedure timing, repetition and clinical parameters with AE risk. Negative binomial regression models were used to calculate incidence rate ratios and 95% confidence intervals. RESULTS: Among 103 participants (median age of 27 years, 97.1% male, 96.1% men who have sex with men), 87 sigmoidoscopies were completed during AHI and 83 at a follow-up visit. Approximately 30 biopsies were obtained per procedure for assessment of colonic viral load and HIV-1 reservoir, immunohistochemistry or phenotypic assays. All 11 AEs were grade 1 (6.5%) and included abdominal discomfort (n = 5, 2.9%), mild rectal bleeding (n = 5, 2.9%) and difficulty passing stool (n = 1, 0.6%). Biopsy-related AE risk was not significantly associated with age, HIV-1 RNA, CD4 T cell count, or number and time of biopsy. CONCLUSIONS: Complications of sigmoidoscopy with biopsy in participants with AHI were infrequent and mild. Longitudinal sampling of the sigmoid colon to evaluate the gut-associated HIV-1 reservoir can be safely performed as part of research studies. CI - (c) 2019 The Authors. Journal of Virus Eradication published by Mediscript. FAU - Chintanaphol, Michelle AU - Chintanaphol M AD - Department of Neurology, Yale University, New Haven, CT, USA. FAU - Sacdalan, Carlo AU - Sacdalan C AD - SEARCH, The Thai Red Cross AIDS Research Centre, Bangkok, Thailand. FAU - Pinyakorn, Suteeraporn AU - Pinyakorn S AD - US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA. AD - Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. FAU - Rerknimitr, Rungsun AU - Rerknimitr R AD - Department of Medicine, Gastroenterology, Chulalongkorn University, Bangkok, Thailand. FAU - Ridtitid, Wiriyaporn AU - Ridtitid W AD - Department of Medicine, Gastroenterology, Chulalongkorn University, Bangkok, Thailand. FAU - Prueksapanich, Piyapan AU - Prueksapanich P AD - Department of Medicine, Gastroenterology, Chulalongkorn University, Bangkok, Thailand. FAU - Sereti, Irini AU - Sereti I AD - Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD, USA. FAU - Schuetz, Alexandra AU - Schuetz A AD - US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA. AD - Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. AD - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. FAU - Crowell, Trevor A AU - Crowell TA AD - US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA. AD - Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. AD - Johns Hopkins University School of Medicine, Baltimore, MD, USA. FAU - Colby, Donn J AU - Colby DJ AD - SEARCH, The Thai Red Cross AIDS Research Centre, Bangkok, Thailand. FAU - Robb, Merlin L AU - Robb ML AD - US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA. AD - Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. FAU - Phanuphak, Nittaya AU - Phanuphak N AD - SEARCH, The Thai Red Cross AIDS Research Centre, Bangkok, Thailand. FAU - Ananworanich, Jintanat AU - Ananworanich J AD - SEARCH, The Thai Red Cross AIDS Research Centre, Bangkok, Thailand. AD - US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA. AD - Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. AD - Department of Global Health, University of Amsterdam, Amsterdam, the Netherlands. FAU - Spudich, Serena S AU - Spudich SS AD - Department of Neurology, Yale University, New Haven, CT, USA. FAU - Kroon, Eugene AU - Kroon E AD - SEARCH, The Thai Red Cross AIDS Research Centre, Bangkok, Thailand. LA - eng PT - Editorial DEP - 20200220 PL - England TA - J Virus Erad JT - Journal of virus eradication JID - 101654142 PMC - PMC7043900 OTO - NOTNLM OT - acute HIV-1 infection OT - colon biopsy OT - research risk EDAT- 2020/03/17 06:00 MHDA- 2020/03/17 06:01 PMCR- 2020/02/20 CRDT- 2020/03/17 06:00 PHST- 2020/03/17 06:00 [entrez] PHST- 2020/03/17 06:00 [pubmed] PHST- 2020/03/17 06:01 [medline] PHST- 2020/02/20 00:00 [pmc-release] PST - epublish SO - J Virus Erad. 2020 Feb 20;6(1):7-10.