PMID- 32175284
OWN - NLM
STAT- MEDLINE
DCOM- 20210319
LR  - 20210319
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 10
DP  - 2020
TI  - Modulatory Effect of Trypanosoma cruzi Infective Stages in Different Dendritic 
      Cell Populations in vitro.
PG  - 20
LID - 10.3389/fcimb.2020.00020 [doi]
LID - 20
AB  - Trypanosoma cruzi is a protozoan parasite that infects at least 7 million persons 
      in the world (OMS, 2019). In endemic areas, infection normally occurs by 
      vectorial transmission; however, outside, it normally happens by blood and 
      includes congenital transmission. The persistence of T. cruzi during infection 
      suggests the presence of immune evasion mechanisms and the modulation of the 
      anti-parasite response to a profile incapable of eradicating the parasite. 
      Dendritic cells (DCs) are a heterogeneous population of antigen-presenting cells 
      (APCs) that patrol tissues with a key role in mediating the interface between the 
      innate and adaptive immune response. Previous results from our lab and other 
      groups have demonstrated that T. cruzi modulates the functional properties of 
      DCs, in vitro and in vivo. During vectorial transmission, metacyclic (m) 
      trypomastigotes (Tps) eliminated along with the insect feces reach the mucous 
      membranes or injured skin. When transmission occurs by the hematic route, the 
      parasite stage involved in the infection is the circulating or blood (b) Tp. 
      Here, we studied in vitro the effect of both infective mTp and bTp in two 
      different populations of DCs, bone marrow-derived DCs (BMDCs) and XS106, a cell 
      line derived from epidermal DCs. Results demonstrated that the interaction of 
      both Tps imparts a different effect in the functionality of these two populations 
      of DCs, suggesting that the stage of T. cruzi and DC maturation status could 
      define the immune response from the beginning of the ingress of the parasite, 
      conditioning the course of the infection.
CI  - Copyright (c) 2020 Gutierrez, Lammel, Ramirez, Gonzalez-Cappa and Poncini.
FAU - Gutierrez, Brenda Celeste
AU  - Gutierrez BC
AD  - Laboratorio de Inmunologia Celular e Inmunopatologia de Infecciones, Instituto de 
      Investigaciones en Microbiologia y Parasitologia Medica (MPaM) UBA-CONICET, 
      Buenos Aires, Argentina.
FAU - Lammel, Estela
AU  - Lammel E
AD  - Laboratorio de Inmunologia Celular e Inmunopatologia de Infecciones, Instituto de 
      Investigaciones en Microbiologia y Parasitologia Medica (MPaM) UBA-CONICET, 
      Buenos Aires, Argentina.
FAU - Ramirez, Marcel Ivan
AU  - Ramirez MI
AD  - Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.
AD  - Departamento de Bioquimica, Universidade Federal Do Parana, Curitiba, Brazil.
FAU - Gonzalez-Cappa, Stella Maris
AU  - Gonzalez-Cappa SM
AD  - Laboratorio de Inmunologia Celular e Inmunopatologia de Infecciones, Instituto de 
      Investigaciones en Microbiologia y Parasitologia Medica (MPaM) UBA-CONICET, 
      Buenos Aires, Argentina.
AD  - Departamento de Microbiologia, Parasitologia e Inmunologia, Facultad de Medicina, 
      Universidad de Buenos Aires, Buenos Aires, Argentina.
FAU - Poncini, Carolina Veronica
AU  - Poncini CV
AD  - Laboratorio de Inmunologia Celular e Inmunopatologia de Infecciones, Instituto de 
      Investigaciones en Microbiologia y Parasitologia Medica (MPaM) UBA-CONICET, 
      Buenos Aires, Argentina.
AD  - Departamento de Microbiologia, Parasitologia e Inmunologia, Facultad de Medicina, 
      Universidad de Buenos Aires, Buenos Aires, Argentina.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200227
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (IL10 protein, mouse)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Cell Line
MH  - Cell Proliferation
MH  - Dendritic Cells/*immunology/metabolism/parasitology
MH  - Interleukin-10/metabolism
MH  - Langerhans Cells/*immunology/metabolism/parasitology
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes/physiology
MH  - Trypanosoma cruzi/growth & development/pathogenicity/*physiology
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC7057235
OTO - NOTNLM
OT  - T cells
OT  - Trypanosoma cruzi
OT  - cell activation
OT  - cytokines
OT  - dendritic cells
EDAT- 2020/03/17 06:00
MHDA- 2021/03/20 06:00
PMCR- 2020/01/01
CRDT- 2020/03/17 06:00
PHST- 2019/09/04 00:00 [received]
PHST- 2020/01/14 00:00 [accepted]
PHST- 2020/03/17 06:00 [entrez]
PHST- 2020/03/17 06:00 [pubmed]
PHST- 2021/03/20 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2020.00020 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2020 Feb 27;10:20. doi: 10.3389/fcimb.2020.00020. 
      eCollection 2020.