PMID- 32175408
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240328
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 8
IP  - 4
DP  - 2020 Feb
TI  - Low expression of A-kinase anchor protein 5 predicts poor prognosis in non-mucin 
      producing stomach adenocarcinoma based on TCGA data.
PG  - 115
LID - 10.21037/atm.2019.12.98 [doi]
LID - 115
AB  - BACKGROUND: In the past, there were not a lot of studies on how A-kinase anchor 
      protein 5 (AKAP5) involving in the pathogenesis and prognosis of non-mucin 
      producing stomach adenocarcinoma (NMSA). Therefore, we studied the relationship 
      between AKAP5 and the prognosis of NMSA and its possible mechanisms using 
      publicly available data from The Cancer Genome Atlas (TCGA). METHODS: RNA 
      high-throughput sequencing and clinicopathologic data of NMSA were downloaded 
      from the TCGA. Clinical pathologic features associated with AKAP5 expression were 
      analyzed using the chi-square and Fisher exact tests. The relationship between 
      the overall survival (OS) and AKAP5 expression was analyzed by the Kaplan-Meier 
      method and the Cox regression analysis. GSEA analysis was performed using the 
      TCGA dataset. RESULTS: Our results indicated that the AKAP5 expression was 
      increased in NMSA (all tumor vs. adjacent mucosa). Also, histologic grade, 
      clinical stage, N classification, and survival status were significantly 
      correlated with AKAP5 expression. Kaplan-Meier curves showed that low AKAP5 
      expression was associated with a poor OS among the NMSA patients (P=5.003e-05), 
      and in the clinical stage III and IV (P=4.646e-05), TNM stage T3 (P=0.016), T4 
      (P=0.001), N2 (P=0.012), N3 (P=0.003), M0 (P=3.911e-05), and histological grade 
      G3 (P=1.658e-04) subgroups. Cox regression analysis showed that reduced AKAP5 
      expression in NMSA is associated with age (HR =1.03, P=0.007), stage (HR =1.84 
      for stage I, II vs. stage III, IV, P=0.002) and M classification (HR =1.8 for M0 
      vs. M1, P=0.010). Gene sets related to cholesterol homeostasis, glycolysis, 
      estrogen response late, adipogenesis, estrogen response early, notch signaling, 
      and peroxisome were differentially enriched with the low AKAP5 expression 
      phenotype. CONCLUSIONS: Low expression of AKAP5 may be a potential molecular 
      marker for predicting poor prognosis of NMSA. Besides, cholesterol homeostasis, 
      glycolysis, estrogen response, adipogenesis, notch signaling, and peroxisome may 
      be the key pathways regulated by AKAP5 in NMSA. It also suggested that AKAP5 
      might potentially have biological functions in the development of stomach 
      adenocarcinoma.
CI  - 2020 Annals of Translational Medicine. All rights reserved.
FAU - Zhong, Zishao
AU  - Zhong Z
AD  - Gastroenterology Department, The Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou 510120, China.
AD  - Gastroenterology Department, Guangdong Provincial Hospital of Chinese Medicine, 
      Guangzhou 510120, China.
FAU - Ye, Zhenhao
AU  - Ye Z
AD  - Gastroenterology Department, The Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou 510120, China.
AD  - Gastroenterology Department, Guangdong Provincial Hospital of Chinese Medicine, 
      Guangzhou 510120, China.
FAU - He, Guihua
AU  - He G
AD  - Gastroenterology Department, The Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou 510120, China.
AD  - Gastroenterology Department, Guangdong Provincial Hospital of Chinese Medicine, 
      Guangzhou 510120, China.
FAU - Zhang, Wang
AU  - Zhang W
AD  - Gastroenterology Department, The Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou 510120, China.
AD  - Gastroenterology Department, Guangdong Provincial Hospital of Chinese Medicine, 
      Guangzhou 510120, China.
FAU - Wang, Jing
AU  - Wang J
AD  - Gastroenterology Department, The Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou 510120, China.
AD  - Gastroenterology Department, Guangdong Provincial Hospital of Chinese Medicine, 
      Guangzhou 510120, China.
FAU - Huang, Suiping
AU  - Huang S
AD  - Gastroenterology Department, The Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou 510120, China.
AD  - Gastroenterology Department, Guangdong Provincial Hospital of Chinese Medicine, 
      Guangzhou 510120, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC7049022
OTO - NOTNLM
OT  - A-kinase anchor protein 5 (AKAP5)
OT  - Stomach neoplasms
OT  - adenocarcinoma
OT  - survival analysis
COIS- Conflicts of Interest: The authors have no conflicts of interest to declare.
EDAT- 2020/03/17 06:00
MHDA- 2020/03/17 06:01
PMCR- 2020/02/01
CRDT- 2020/03/17 06:00
PHST- 2020/03/17 06:00 [entrez]
PHST- 2020/03/17 06:00 [pubmed]
PHST- 2020/03/17 06:01 [medline]
PHST- 2020/02/01 00:00 [pmc-release]
AID - atm-08-04-115 [pii]
AID - 10.21037/atm.2019.12.98 [doi]
PST - ppublish
SO  - Ann Transl Med. 2020 Feb;8(4):115. doi: 10.21037/atm.2019.12.98.