PMID- 32175785
OWN - NLM
STAT- MEDLINE
DCOM- 20210106
LR  - 20210106
IS  - 1544-0591 (Electronic)
IS  - 0022-0345 (Linking)
VI  - 99
IP  - 6
DP  - 2020 Jun
TI  - Subgingival Host-Microbial Interactions in Hyperglycemic Individuals.
PG  - 650-657
LID - 10.1177/0022034520906842 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is an established risk factor for periodontitis, 
      yet its contribution to creating host-bacterial disequilibrium in the subgingival 
      crevice is poorly understood. The present investigation aimed to quantify the 
      impact of hyperglycemia on host-bacterial interactions in established 
      periodontitis and to map shifts in these dynamics following mechanical 
      nonsurgical therapy. Seventeen T2DM and 17 non-T2DM subjects with generalized 
      severe chronic periodontitis were recruited along with 20 periodontally healthy 
      individuals. Subjects with periodontitis were treated with scaling and root 
      planing (SRP). Samples of subgingival biofilm and gingival crevicular fluid were 
      collected at baseline and at 1-, 3-, and 6 mo postoperatively. Correlations were 
      generated between 13.7 million 16S ribosomal DNA sequences and 8 immune 
      mediators. Intermicrobial and host-microbial interactions were modeled using 
      differential network analysis. Periodontal health was characterized by a sparse 
      interbacterial and highly connected cytokine-bacterial network, while both 
      normoglycemics and T2DM subjects with periodontitis demonstrated robust 
      congeneric and intergeneric hubs but significantly fewer cytokine-bacterial 
      connections. Following SRP, the cytokine-bacterial edges demonstrated a 2-fold 
      increase 1 mo postoperatively and a 10-fold increase at 6 mo in normoglycemics. 
      In hyperglycemics, there was a doubling at 1 mo but no further changes 
      thereafter. These shifts accompanied an increasingly sparse interbacterial 
      network. In normoglycemics, the nodes anchored by interleukin (IL)-4, IL-6, and 
      IL-10 demonstrated greatest rewiring, while in hyperglycemics, IL-1beta, IL-6, 
      INF-gamma, and IL-17 exhibited progressive rewiring. Thus, the present investigation 
      points to a breakdown in host-bacterial mutualism in periodontitis, with 
      interbacterial interactions rather than host-bacterial interactions primarily 
      determining community assembly. Hyperglycemia further exacerbates this uncoupled 
      mutualism. Our data also demonstrate that while nonsurgical therapy might not 
      consistently alter microbial abundances or lower proinflammatory molecules, it 
      "reboots" the interaction between the immunoinflammatory system and the newly 
      colonizing microbiome, restoring a role for the immune system in determining 
      bacterial colonization. However, this outcome is lower and delayed in 
      hyperglycemics.
FAU - Kumar, P S
AU  - Kumar PS
AUID- ORCID: 0000-0001-5844-1341
AD  - Division of Periodontology, College of Dentistry, The Ohio State University, 
      Columbus, OH, USA.
FAU - Monteiro, M F
AU  - Monteiro MF
AD  - Division of Periodontology, Piracicaba Dental School, University of Campinas, 
      Piracicaba, Brazil.
FAU - Dabdoub, S M
AU  - Dabdoub SM
AUID- ORCID: 0000-0002-1374-8347
AD  - Division of Periodontology, College of Dentistry, The Ohio State University, 
      Columbus, OH, USA.
FAU - Miranda, G L
AU  - Miranda GL
AD  - Division of Periodontology, School of Dentistry, Paulista University, Sao Paulo, 
      Brazil.
FAU - Casati, M Z
AU  - Casati MZ
AD  - Division of Periodontology, Piracicaba Dental School, University of Campinas, 
      Piracicaba, Brazil.
AD  - Division of Periodontology, School of Dentistry, Paulista University, Sao Paulo, 
      Brazil.
FAU - Ribeiro, F V
AU  - Ribeiro FV
AD  - Division of Periodontology, School of Dentistry, Paulista University, Sao Paulo, 
      Brazil.
FAU - Cirano, F R
AU  - Cirano FR
AD  - Division of Periodontology, School of Dentistry, Paulista University, Sao Paulo, 
      Brazil.
FAU - Pimentel, S P
AU  - Pimentel SP
AD  - Division of Periodontology, School of Dentistry, Paulista University, Sao Paulo, 
      Brazil.
FAU - Casarin, R C V
AU  - Casarin RCV
AUID- ORCID: 0000-0003-1743-5855
AD  - Division of Periodontology, Piracicaba Dental School, University of Campinas, 
      Piracicaba, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200316
PL  - United States
TA  - J Dent Res
JT  - Journal of dental research
JID - 0354343
SB  - IM
MH  - Chronic Periodontitis/therapy
MH  - Dental Scaling
MH  - Diabetes Mellitus, Type 2
MH  - Gingival Crevicular Fluid
MH  - Humans
MH  - *Microbial Interactions
MH  - Root Planing
OTO - NOTNLM
OT  - 16S
OT  - cytokine
OT  - diabetes
OT  - interactome
OT  - microbiome
OT  - periodontitis
EDAT- 2020/03/17 06:00
MHDA- 2021/01/07 06:00
CRDT- 2020/03/17 06:00
PHST- 2020/03/17 06:00 [pubmed]
PHST- 2021/01/07 06:00 [medline]
PHST- 2020/03/17 06:00 [entrez]
AID - 10.1177/0022034520906842 [doi]
PST - ppublish
SO  - J Dent Res. 2020 Jun;99(6):650-657. doi: 10.1177/0022034520906842. Epub 2020 Mar 
      16.