PMID- 32176389 OWN - NLM STAT- MEDLINE DCOM- 20200806 LR - 20200806 IS - 1600-0714 (Electronic) IS - 0904-2512 (Linking) VI - 49 IP - 4 DP - 2020 Apr TI - Regulatory effect of 17beta-estradiol on the expression of beta-defensin-2 and proinflammatory cytokines in human oral epithelial cells. PG - 365-372 LID - 10.1111/jop.13016 [doi] AB - BACKGROUND: Although estrogen deficiency has been proposed as a risk factor for oral mucosal inflammatory diseases in post-menopausal women, the mechanisms involved remain unclear. This study aimed to investigate the effect of 17beta-estradiol (E2) on the inflammatory response stimulated by interleukin-1 beta (IL-1beta) in human oral mucosal epithelial cells (hOMECs) and its possible mechanism. METHODS: Primary hOMECs were obtained from female infants and cultured in keratinocyte growth medium. The hOMECs at second passage were collected and stimulated by 10(-7) mol/L ICI182,780 or 10(-7) mol/L G1 for 1 hour, E2 (10(-7) mol/L, 10(-8) mol/L, 10(-9) mol/L) for 36 hour, 100 ng/mL IL-1beta for 12 hours, respectively. Human beta-2 defensin (hBD-2), tumor necrosis factor-alpha (TNF)-alpha, IL-6, IL-8, estrogen receptor-alpha (ERalpha), estrogen receptor-beta (ERbeta), and G protein-coupled receptor 30 (GPR30) mRNA levels and protein levels were measured by real-time quantitative polymerase chain reaction (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), and Western Blot (WB), respectively. RESULTS: Expression of hBD-2 and inflammatory cytokines increased after IL-1beta stimulation, which was down-regulated by E2 pre-treatment. With ICI182,780, the suppression of E2 on hBD-2 mRNA was attenuated. With G1, the mRNA expression and protein expression of hBD-2 were reduced. CONCLUSION: Pre-treatment of hOMECs with E2 at physiological concentrations inhibited the IL-1beta-induced expression of hBD-2 and inflammatory cytokines. The protective effects of E2 suggest its potential use treating oral inflammatory diseases in clinical practice. CI - (c) 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. FAU - Wu, Tiantian AU - Wu T AUID- ORCID: 0000-0002-0843-5186 AD - Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China. AD - Guangxi Key Laboratory of AIDS Prevention and Treatment, Guangxi Medical University, Nanning, China. AD - Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China. FAU - Tang, Caijin AU - Tang C AD - Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China. AD - Guangxi Key Laboratory of AIDS Prevention and Treatment, Guangxi Medical University, Nanning, China. AD - Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China. FAU - Chen, Yi AU - Chen Y AD - Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China. AD - Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China. FAU - Yong, Xiangzhi AU - Yong X AD - Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China. AD - Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China. FAU - Liu, Zhenmin AU - Liu Z AD - Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China. FAU - Jiang, Lanlan AU - Jiang L AD - Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China. AD - Guangxi Key Laboratory of AIDS Prevention and Treatment, Guangxi Medical University, Nanning, China. FAU - Zeng, Qixin AU - Zeng Q AD - Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China. AD - Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China. FAU - Tao, Renchuan AU - Tao R AUID- ORCID: 0000-0001-8551-0031 AD - Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China. AD - Guangxi Key Laboratory of AIDS Prevention and Treatment, Guangxi Medical University, Nanning, China. AD - Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities and Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China. LA - eng GR - 81771073/National Natural Science Foundation of China/ GR - 2015GXNSFDA139018/Guangxi Natural Science Foundation/ GR - YCSW2019105/Innovation Project of Guangxi Graduate Education/ PT - Journal Article DEP - 20200414 PL - Denmark TA - J Oral Pathol Med JT - Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology JID - 8911934 RN - 0 (Cytokines) RN - 0 (DEFB4A protein, human) RN - 0 (beta-Defensins) RN - 4TI98Z838E (Estradiol) SB - IM MH - Cells, Cultured MH - Cytokines/*immunology MH - Epithelial Cells/*drug effects/immunology MH - Estradiol/*pharmacology MH - Female MH - Humans MH - beta-Defensins/*immunology OTO - NOTNLM OT - 17beta-estradiol OT - estrogen receptors OT - human oral mucosal epithelial cells OT - inflammatory cytokines OT - regulatory effect EDAT- 2020/03/17 06:00 MHDA- 2020/08/07 06:00 CRDT- 2020/03/17 06:00 PHST- 2019/11/25 00:00 [received] PHST- 2020/02/24 00:00 [revised] PHST- 2020/03/10 00:00 [accepted] PHST- 2020/03/17 06:00 [pubmed] PHST- 2020/08/07 06:00 [medline] PHST- 2020/03/17 06:00 [entrez] AID - 10.1111/jop.13016 [doi] PST - ppublish SO - J Oral Pathol Med. 2020 Apr;49(4):365-372. doi: 10.1111/jop.13016. Epub 2020 Apr 14.