PMID- 32178274
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2304-8158 (Print)
IS  - 2304-8158 (Electronic)
IS  - 2304-8158 (Linking)
VI  - 9
IP  - 3
DP  - 2020 Mar 12
TI  - Development of a DNA-Based Detection Method for Cocos Nucifera Using TaqMan 
      Real-Time PCR.
LID - 10.3390/foods9030332 [doi]
LID - 332
AB  - So far, only a few cases of immunoglobulin E (IgE)-mediated coconut allergies 
      have been described in the literature. Due to a growing consumption of 
      coconut-containing foods in occidental countries, the number of coconut allergies 
      may also increase. As there is no causative immunotherapy in clinical routine, 
      appropriate food labelling is particularly important, also with regard to 
      cross-contamination, to prevent serious health consequences. The purpose of this 
      study was to develop a DNA-based detection method for coconut (Cocos nucifera). 
      Initially, three sets of coconut-specific primers were designed and tested. A 
      TaqMan probe was then developed to identify and quantify coconut by real-time 
      PCR assay. With 27 other plant and animal species, the specificity of the 
      primer/probe system was tested and cross reactivity was excluded. In a dilution 
      series, a limit of detection of 1 pg/microL was determined. Thus, the developed 
      real-time PCR assay is a suitable method to detect coconut in food.
FAU - Wrage, Jasmin
AU  - Wrage J
AD  - GALAB Laboratories GmbH, Am Schleusengraben 7, 21029 Hamburg, Germany.
AD  - Institute of Food Chemistry, Hamburg School of Food Science, University of 
      Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
FAU - Kleyner, Oxana
AU  - Kleyner O
AD  - GALAB Laboratories GmbH, Am Schleusengraben 7, 21029 Hamburg, Germany.
FAU - Rohn, Sascha
AU  - Rohn S
AD  - Institute of Food Chemistry, Hamburg School of Food Science, University of 
      Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
FAU - Kuballa, Jurgen
AU  - Kuballa J
AUID- ORCID: 0000-0001-6336-9452
AD  - GALAB Laboratories GmbH, Am Schleusengraben 7, 21029 Hamburg, Germany.
LA  - eng
GR  - FKZ 281601914/Bundesministerium fur Ernahrung und Landwirtschaft/
PT  - Journal Article
DEP - 20200312
PL  - Switzerland
TA  - Foods
JT  - Foods (Basel, Switzerland)
JID - 101670569
PMC - PMC7143811
OTO - NOTNLM
OT  - Cocos nucifera
OT  - TaqMan probe
OT  - coconut allergy
OT  - primer design
OT  - real-time PCR
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/03/18 06:00
MHDA- 2020/03/18 06:01
PMCR- 2020/03/12
CRDT- 2020/03/18 06:00
PHST- 2020/02/09 00:00 [received]
PHST- 2020/02/24 00:00 [revised]
PHST- 2020/03/09 00:00 [accepted]
PHST- 2020/03/18 06:00 [entrez]
PHST- 2020/03/18 06:00 [pubmed]
PHST- 2020/03/18 06:01 [medline]
PHST- 2020/03/12 00:00 [pmc-release]
AID - foods9030332 [pii]
AID - foods-09-00332 [pii]
AID - 10.3390/foods9030332 [doi]
PST - epublish
SO  - Foods. 2020 Mar 12;9(3):332. doi: 10.3390/foods9030332.