PMID- 32179226
OWN - NLM
STAT- MEDLINE
DCOM- 20200710
LR  - 20200710
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 262
DP  - 2020 Jul
TI  - ROS-mediated programmed cell death (PCD) of Thalassiosira pseudonana under the 
      stress of BDE-47.
PG  - 114342
LID - S0269-7491(19)37538-4 [pii]
LID - 10.1016/j.envpol.2020.114342 [doi]
AB  - Polybrominated diphenyl ethers (PBDEs) are a series of highly persistent organic 
      pollutants (POPs) ubiquitously distributed in marine environments. As key primary 
      producers, microalgae are the start of PBDEs bioaccumulations and vulnerable to 
      their toxicities. In order to deeply investigate the toxic mechanism of PBDEs on 
      microalgal cells, the occurrence of programmed cell death (PCD) in a model diatom 
      Thalassiosira pseudonana and its possible mediating mechanism were studied. The 
      results indicated: cell death of T. pseudonana happened under the stress of 
      BDE-47, which was proved to be PCD based on the correlations with three 
      biochemical markers (DNA fragmentation, phosphatidylserine externalization and 
      caspase activity) and three molecular markers [Metacaspase 2 gene (TpMC2), 
      Death-associated protein gene (DAP3) and Death-specific protein 1 gene (TpDSP1)]; 
      Furthermore, the changes of cellular ROS levels were correlated with the PCD 
      markers and the dead cell rates, and the cell membrane and the chloroplast were 
      identified as the major ROS production sites. Therefore, we concluded that PCD 
      might be an important toxic mechanism of PBDEs on microalgal cells, and that 
      chloroplast- and cell membrane-produced ROS was an important signaling molecule 
      to mediate the PCD activation process. Our research firstly indicated microalgal 
      PCD could be induced by PBDEs, and increased our knowledge of the toxic 
      mechanisms by which POPs affect microalgal cells.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Zhao, Yirong
AU  - Zhao Y
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Tang, Xuexi
AU  - Tang X
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China; Laboratory for Marine Ecology and Environmental 
      Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 
      266071, China.
FAU - Qu, Fangyuan
AU  - Qu F
AD  - First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, 
      China.
FAU - Lv, Mengchen
AU  - Lv M
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Liu, Qian
AU  - Liu Q
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Li, Jun
AU  - Li J
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Li, Luying
AU  - Li L
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Zhang, Bihan
AU  - Zhang B
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Zhao, Yan
AU  - Zhao Y
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China; Laboratory for Marine Ecology and Environmental 
      Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 
      266071, China. Electronic address: zhaoyan@ouc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200306
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - 0 (Reactive Oxygen Species)
RN  - 0N97R5X10X (2,2',4,4'-tetrabromodiphenyl ether)
SB  - IM
MH  - Apoptosis
MH  - *Diatoms
MH  - *Halogenated Diphenyl Ethers
MH  - Reactive Oxygen Species
OTO - NOTNLM
OT  - BDE-47
OT  - Programmed cell death
OT  - ROS
OT  - Thalassiosira pseudonana
OT  - Toxic mechanisms
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/03/18 06:00
MHDA- 2020/07/11 06:00
CRDT- 2020/03/18 06:00
PHST- 2019/12/16 00:00 [received]
PHST- 2020/02/21 00:00 [revised]
PHST- 2020/03/05 00:00 [accepted]
PHST- 2020/03/18 06:00 [pubmed]
PHST- 2020/07/11 06:00 [medline]
PHST- 2020/03/18 06:00 [entrez]
AID - S0269-7491(19)37538-4 [pii]
AID - 10.1016/j.envpol.2020.114342 [doi]
PST - ppublish
SO  - Environ Pollut. 2020 Jul;262:114342. doi: 10.1016/j.envpol.2020.114342. Epub 2020 
      Mar 6.