PMID- 32179476
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210110
IS  - 2589-0042 (Electronic)
IS  - 2589-0042 (Print)
IS  - 2589-0042 (Linking)
VI  - 23
IP  - 3
DP  - 2020 Mar 27
TI  - Cytosolic Ca(2+) Modulates Golgi Structure Through PKCalpha-Mediated GRASP55 
      Phosphorylation.
PG  - 100952
LID - S2589-0042(20)30136-X [pii]
LID - 10.1016/j.isci.2020.100952 [doi]
LID - 100952
AB  - It has been well documented that the ER responds to cellular stresses through the 
      unfolded protein response (UPR), but it is unknown how the Golgi responds to 
      similar stresses. In this study, we treated HeLa cells with ER stress inducers, 
      thapsigargin (TG), tunicamycin (Tm), and dithiothreitol (DTT), and found that 
      only TG treatment resulted in Golgi fragmentation. TG induced Golgi fragmentation 
      at a low dose and short time when UPR was undetectable, indicating that Golgi 
      fragmentation occurs independently of ER stress. Further experiments demonstrated 
      that TG induces Golgi fragmentation through elevating intracellular Ca(2+) and 
      protein kinase Calpha (PKCalpha) activity, which phosphorylates the Golgi stacking 
      protein GRASP55. Significantly, activation of PKCalpha with other activating or 
      inflammatory agents, including phorbol 12-myristate 13-acetate and histamine, 
      modulates Golgi structure in a similar fashion. Hence, our study revealed a novel 
      mechanism through which increased cytosolic Ca(2+) modulates Golgi structure and 
      function.
CI  - Copyright (c) 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Ireland, Stephen
AU  - Ireland S
AD  - Department of Molecular, Cellular and Developmental Biology, University of 
      Michigan, Biological Sciences Building, 1105 North University Avenue, Ann Arbor, 
      MI 48109-1085, USA.
FAU - Ramnarayanan, Saiprasad
AU  - Ramnarayanan S
AD  - Department of Molecular, Cellular and Developmental Biology, University of 
      Michigan, Biological Sciences Building, 1105 North University Avenue, Ann Arbor, 
      MI 48109-1085, USA.
FAU - Fu, Mingzhou
AU  - Fu M
AD  - Department of Molecular, Cellular and Developmental Biology, University of 
      Michigan, Biological Sciences Building, 1105 North University Avenue, Ann Arbor, 
      MI 48109-1085, USA.
FAU - Zhang, Xiaoyan
AU  - Zhang X
AD  - Department of Molecular, Cellular and Developmental Biology, University of 
      Michigan, Biological Sciences Building, 1105 North University Avenue, Ann Arbor, 
      MI 48109-1085, USA.
FAU - Zhang, Jianchao
AU  - Zhang J
AD  - Department of Molecular, Cellular and Developmental Biology, University of 
      Michigan, Biological Sciences Building, 1105 North University Avenue, Ann Arbor, 
      MI 48109-1085, USA.
FAU - Li, Jie
AU  - Li J
AD  - Department of Molecular, Cellular and Developmental Biology, University of 
      Michigan, Biological Sciences Building, 1105 North University Avenue, Ann Arbor, 
      MI 48109-1085, USA.
FAU - Emebo, Dabel
AU  - Emebo D
AD  - Department of Molecular, Cellular and Developmental Biology, University of 
      Michigan, Biological Sciences Building, 1105 North University Avenue, Ann Arbor, 
      MI 48109-1085, USA.
FAU - Wang, Yanzhuang
AU  - Wang Y
AD  - Department of Molecular, Cellular and Developmental Biology, University of 
      Michigan, Biological Sciences Building, 1105 North University Avenue, Ann Arbor, 
      MI 48109-1085, USA; Department of Neurology, University of Michigan School of 
      Medicine, Ann Arbor, MI 48109-1085, USA. Electronic address: yzwang@umich.edu.
LA  - eng
GR  - R35 GM130331/GM/NIGMS NIH HHS/United States
GR  - R56 AG062225/AG/NIA NIH HHS/United States
PT  - Journal Article
DEP - 20200228
PL  - United States
TA  - iScience
JT  - iScience
JID - 101724038
PMC - PMC7078314
OTO - NOTNLM
OT  - Biological Sciences
OT  - Cell Biology
OT  - Functional Aspects of Cell Biology
COIS- Declaration of Interests The authors declare no competing financial interests.
EDAT- 2020/03/18 06:00
MHDA- 2020/03/18 06:01
PMCR- 2020/02/28
CRDT- 2020/03/18 06:00
PHST- 2019/10/28 00:00 [received]
PHST- 2020/01/31 00:00 [revised]
PHST- 2020/02/25 00:00 [accepted]
PHST- 2020/03/18 06:00 [pubmed]
PHST- 2020/03/18 06:01 [medline]
PHST- 2020/03/18 06:00 [entrez]
PHST- 2020/02/28 00:00 [pmc-release]
AID - S2589-0042(20)30136-X [pii]
AID - 100952 [pii]
AID - 10.1016/j.isci.2020.100952 [doi]
PST - ppublish
SO  - iScience. 2020 Mar 27;23(3):100952. doi: 10.1016/j.isci.2020.100952. Epub 2020 
      Feb 28.