PMID- 32180714 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200928 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 11 DP - 2020 TI - Genetic Diversity of HLA Class I and Class II Alleles in Thai Populations: Contribution to Genotype-Guided Therapeutics. PG - 78 LID - 10.3389/fphar.2020.00078 [doi] LID - 78 AB - Human leukocyte antigen (HLA) class I and II are known to have association with severe cutaneous adverse reactions (SCARs) when exposing to certain drug treatment. Due to genetic differences at population level, drug hypersensitivity reactions are varied, and thus common pharmacogenetics markers for one country might be different from another country, for instance, HLA-A*31:01 is associated with carbamazepine (CBZ)-induced SCARs in European and Japanese while HLA-B*15:02 is associated with CBZ-induced Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN) among Taiwanese and Southeast Asian. Such differences pose a major challenge to prevent drug hypersensitivity when pharmacogenetics cannot be ubiquitously and efficiently translated into clinic. Therefore, a population-wide study of the distribution of HLA-pharmacogenetics markers is needed. This work presents a study of Thai HLA alleles on both HLA class I and II genes from 470 unrelated Thai individuals by means of polymerase chain reaction sequence-specific oligonucleotide (PCR-SSO) in which oligonucleotide probes along the stretches of HLA-A, -B, -C, -DRB1, -DQA1, and -DQB1 genes were genotyped. These 470 individuals were selected according to their regional locations, which were from North, Northeast, South, Central, and a capital city, Bangkok. Top ranked HLA alleles in Thai population include HLA-A*11:01 (26.06%), -B*46:01 (14.04%), -C* 01:02 (17.13%), -DRB1*12:02 (15.32%), -DQA1*01:01 (24.89%), and -DQB1*05:02 (21.28%). The results revealed that the distribution of HLA-pharmacogenetics alleles from the South had more HLA-B75 family that a typical HLA-B*15:02 pharmacogenetics test for SJS/TEN screening would not cover. Besides the view across the nation, when compared HLA alleles from Thai population with HLA alleles from both European and Asian countries, the distribution landscape of HLA-associated drug hypersensitivity across many countries could be observed. Consequently, this pharmacogenetics database offers a comprehensive view of pharmacogenetics marker distribution in Thailand that could be used as a reference for other Southeast Asian countries to validate the feasibility of their future pharmacogenetics deployment. CI - Copyright (c) 2020 Satapornpong, Jinda, Jantararoungtong, Koomdee, Chaichan, Pratoomwun, Na Nakorn, Aekplakorn, Wilantho, Ngamphiw, Tongsima and Sukasem. FAU - Satapornpong, Patompong AU - Satapornpong P AD - Division of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. AD - Laboratory for Pharmacogenomics, Somdech Phra Debaratana Medical Center (SDMC), Ramathibodi Hospital, Bangkok, Thailand. AD - Division of General Pharmacy Practice, Department of Pharmaceutical Care, College of Pharmacy, Rangsit University, Pathum Thani, Thailand. FAU - Jinda, Pimonpan AU - Jinda P AD - Division of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. AD - Laboratory for Pharmacogenomics, Somdech Phra Debaratana Medical Center (SDMC), Ramathibodi Hospital, Bangkok, Thailand. FAU - Jantararoungtong, Thawinee AU - Jantararoungtong T AD - Division of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. AD - Laboratory for Pharmacogenomics, Somdech Phra Debaratana Medical Center (SDMC), Ramathibodi Hospital, Bangkok, Thailand. FAU - Koomdee, Napatrupron AU - Koomdee N AD - Division of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. AD - Laboratory for Pharmacogenomics, Somdech Phra Debaratana Medical Center (SDMC), Ramathibodi Hospital, Bangkok, Thailand. FAU - Chaichan, Chonlawat AU - Chaichan C AD - Division of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. AD - Laboratory for Pharmacogenomics, Somdech Phra Debaratana Medical Center (SDMC), Ramathibodi Hospital, Bangkok, Thailand. FAU - Pratoomwun, Jirawat AU - Pratoomwun J AD - Division of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. AD - Laboratory for Pharmacogenomics, Somdech Phra Debaratana Medical Center (SDMC), Ramathibodi Hospital, Bangkok, Thailand. FAU - Na Nakorn, Chalitpon AU - Na Nakorn C AD - Graduate Program in Translational Medicine, Research Center, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. FAU - Aekplakorn, Wichai AU - Aekplakorn W AD - Department of Community Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. FAU - Wilantho, Alisa AU - Wilantho A AD - National Biobank of Thailand, National Science and Technology Development Agency, Pathum Thani, Thailand. AD - National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand. FAU - Ngamphiw, Chumpol AU - Ngamphiw C AD - National Biobank of Thailand, National Science and Technology Development Agency, Pathum Thani, Thailand. AD - National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand. FAU - Tongsima, Sissades AU - Tongsima S AD - National Biobank of Thailand, National Science and Technology Development Agency, Pathum Thani, Thailand. AD - National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand. FAU - Sukasem, Chonlaphat AU - Sukasem C AD - Division of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. AD - Laboratory for Pharmacogenomics, Somdech Phra Debaratana Medical Center (SDMC), Ramathibodi Hospital, Bangkok, Thailand. AD - The Thai Severe Cutaneous Adverse Drug Reaction (THAI-SCAR) Research Group, Bangkok, Thailand. LA - eng PT - Journal Article DEP - 20200227 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC7057685 OTO - NOTNLM OT - HLA class I OT - HLA class II OT - Thai population OT - human leukocyte antigen OT - pharmacogenetic marker EDAT- 2020/03/18 06:00 MHDA- 2020/03/18 06:01 PMCR- 2020/02/27 CRDT- 2020/03/18 06:00 PHST- 2019/06/23 00:00 [received] PHST- 2020/01/27 00:00 [accepted] PHST- 2020/03/18 06:00 [entrez] PHST- 2020/03/18 06:00 [pubmed] PHST- 2020/03/18 06:01 [medline] PHST- 2020/02/27 00:00 [pmc-release] AID - 10.3389/fphar.2020.00078 [doi] PST - epublish SO - Front Pharmacol. 2020 Feb 27;11:78. doi: 10.3389/fphar.2020.00078. eCollection 2020.