PMID- 32181159
OWN - NLM
STAT- MEDLINE
DCOM- 20210317
LR  - 20210317
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 10
DP  - 2020
TI  - Dendritic Cell Maturation Regulates TSPAN7 Function in HIV-1 Transfer to CD4(+) T 
      Lymphocytes.
PG  - 70
LID - 10.3389/fcimb.2020.00070 [doi]
LID - 70
AB  - Dendritic cells (DCs) serve a key function in host defense, linking innate 
      detection of microbes to activation of pathogen-specific adaptive immune 
      responses. DCs express cell surface receptors for HIV-1 entry, but are relatively 
      resistant to productive viral replication. They do, however, facilitate infection 
      of co-cultured T-helper cells through a process referred to as trans-infection. 
      We previously showed that tetraspanin 7 (TSPAN7), a transmembrane protein, is 
      involved, through positive regulation of actin nucleation, in the transfer of 
      HIV-1 from the dendrites of immature monocyte-derived DCs (iMDDCs) to activated 
      CD4(+) T lymphocytes. Various molecular mechanisms have been described regarding 
      HIV-1 trans-infection and seem to depend on DC maturation status. We sought to 
      investigate the crosstalk between DC maturation status, TSPAN7 expression and 
      trans-infection. We followed trans-infection through co-culture of iMDDCs with 
      CD4(+) T lymphocytes, in the presence of CXCR4-tropic replicative-competent HIV-1 
      expressing GFP. T cell infection, DC maturation status and dendrite morphogenesis 
      were assessed through time both by flow cytometry and confocal microscopy. Our 
      previously described TSPAN7/actin nucleation-dependent mechanism of HIV-1 
      transfer appeared to be mostly observed during the first 20 h of co-culture 
      experiments and to be independent of HIV replication. In the course of co-culture 
      experiments, we observed a progressive maturation of MDDCs, correlated with a 
      decrease in TSPAN7 expression, a drastic loss of dendrites and a change in the 
      shape of DCs. A TSPAN7 and actin nucleation-independent mechanism of 
      trans-infection, relying on HIV-1 replication, was then at play. We discovered 
      that TSPAN7 expression is downregulated in response to different innate immune 
      stimuli driving DC maturation, explaining the requirement for a TSPAN7/actin 
      nucleation-independent mechanism of HIV transfer from mature MDDCs (mMDDCs) to T 
      lymphocytes. As previously described, this mechanism relies on the capture of 
      HIV-1 by the I-type lectin CD169/Siglec-1 on mMDDCs and the formation of a "big 
      invaginated pocket" at the surface of DCs, both events being tightly regulated by 
      DC maturation. Interestingly, in iMDDCs, although CD169/Siglec-1 can capture 
      HIV-1, this capture does not lead to HIV-1 transfer to T lymphocytes.
CI  - Copyright (c) 2020 Perot, Garcia-Paredes, Luka and Menager.
FAU - Perot, Brieuc P
AU  - Perot BP
AD  - Inflammatory Responses and Transcriptomic Networks in Diseases, Institut Imagine, 
      Paris, France.
AD  - Inserm U1163, Paris, France.
FAU - Garcia-Paredes, Victor
AU  - Garcia-Paredes V
AD  - Inflammatory Responses and Transcriptomic Networks in Diseases, Institut Imagine, 
      Paris, France.
AD  - Inserm U1163, Paris, France.
FAU - Luka, Marine
AU  - Luka M
AD  - Inflammatory Responses and Transcriptomic Networks in Diseases, Institut Imagine, 
      Paris, France.
AD  - Inserm U1163, Paris, France.
FAU - Menager, Mickael M
AU  - Menager MM
AD  - Inflammatory Responses and Transcriptomic Networks in Diseases, Institut Imagine, 
      Paris, France.
AD  - Inserm U1163, Paris, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200228
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (SIGLEC1 protein, human)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 1)
RN  - 0 (TSPAN7 protein, human)
RN  - 0 (Tetraspanins)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/*immunology/*virology
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Dendrites/physiology
MH  - Dendritic Cells/*physiology
MH  - HEK293 Cells
MH  - HIV Infections/*immunology
MH  - HIV-1
MH  - Humans
MH  - Monocytes/immunology/virology
MH  - Nerve Tissue Proteins/genetics/*immunology
MH  - Sialic Acid Binding Ig-like Lectin 1/immunology
MH  - Tetraspanins/genetics/*immunology
MH  - Time Factors
MH  - Transduction, Genetic
PMC - PMC7059179
OTO - NOTNLM
OT  - HIV-1
OT  - TSPAN7
OT  - actin nucleation
OT  - dendritic cell maturation
OT  - kinetic of transfer
OT  - trans-infection
EDAT- 2020/03/18 06:00
MHDA- 2021/03/18 06:00
PMCR- 2020/01/01
CRDT- 2020/03/18 06:00
PHST- 2019/11/26 00:00 [received]
PHST- 2020/02/12 00:00 [accepted]
PHST- 2020/03/18 06:00 [entrez]
PHST- 2020/03/18 06:00 [pubmed]
PHST- 2021/03/18 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2020.00070 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2020 Feb 28;10:70. doi: 10.3389/fcimb.2020.00070. 
      eCollection 2020.